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OfflineSirPsycho
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: Melgo] * 8
    #28391198 - 07/10/23 09:44 AM (6 months, 16 days ago)

They are starting to sporulate. Zoom in on the gills of the 2nd tallest fruit (centre of photo) to see patches of spores forming.



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OnlineThe Tao
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: SirPsycho]
    #28391256 - 07/10/23 11:19 AM (6 months, 16 days ago)

Nice. Noticing any variations between indoor and outdoor grows? I saw the 1 1/2” cap and it seemed like the caps started curling pretty quickly. I know these beasties do whatever they want but I have a couple of spent cakes and I’m setting up to try this.


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OfflineSirPsycho
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: The Tao] * 1
    #28391263 - 07/10/23 11:38 AM (6 months, 16 days ago)

Quote:

The Tao said:
Nice. Noticing any variations between indoor and outdoor grows? I saw the 1 1/2” cap and it seemed like the caps started curling pretty quickly. I know these beasties do whatever they want but I have a couple of spent cakes and I’m setting up to try this.



Shorter, fatter stipe, bigger caps. Curling is about the same as indoors or a bit more tame


--------------------
:pm:Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints:pm:
Balance in life is like running on ice.

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OfflineMelgo
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: SirPsycho] * 4
    #28391359 - 07/10/23 01:45 PM (6 months, 16 days ago)

Quoting myself from my journal, because that might be an interesting discussion to be had here.

Quote:

Melgo said:
Alright, I’m starting to doubt the “slow” vs “fast” lineage theory now :confused2:

The photo below, is the result of a print I received from 0t0lerance. That print was HUGE, and to quote him: “It's a lineage that goes all the way back to workman (via TCO via CaptainFuture)”. :mushroom2:

I simply followed my standard procedure except for: Spawn/Sub ratio is 3/5 - (Rye/CV) & I added a tablespoon of powdered oyster shells per jar of cactus soil casing to try to adjust the ph (don't think it was enough tho').

I had blobs forming at day 10 after spawning the shoebox. Actual pins at day 18 I think. So for me it behaved EXACTLY as with the “fast” lineage I already have, you can look up photos from the procedure, same colors, same spaghetti shoebox.

Enough talk, here’s the photo:


Next step will be to trip with these and see how strong they are.

I don’t doubt 0t0lerance for a second on the fact that it’s the “slow” lineage. And actually, a couple of weeks after receiving 0t0lerance’s print I’ve received a print from Konbri, same thing: “slow” lineage. Both 0t0lerance & Konbri have grown it and shared photos and experiences.

I’ve grown Konbri’s print as well – shoebox, same parameters as above - here’s the result 20 days after spawning it (and it behaved exactly as the few lines written above):


The Semperviva/Subtropicalis mystery continues… :shrug:


PS: Since 0t0lerance's print was so big, instead of hoarding it like a dragon, I've cut it in 4 and already sent 2 quarters to 2 other Shroomery members here. A third one will go out this week, so we'll have more trials with this one at some point :pm:




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Offlinechris77
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: Melgo] * 2
    #28391371 - 07/10/23 01:54 PM (6 months, 16 days ago)

Quote:

Melgo said:
Quoting myself from my journal, because that might be an interesting discussion to be had here.

Quote:

Melgo said:
Alright, I’m starting to doubt the “slow” vs “fast” lineage theory now :confused2:

The photo below, is the result of a print I received from 0t0lerance. That print was HUGE, and to quote him: “It's a lineage that goes all the way back to workman (via TCO via CaptainFuture)”. :mushroom2:

I simply followed my standard procedure except for: Spawn/Sub ratio is 3/5 - (Rye/CV) & I added a tablespoon of powdered oyster shells per jar of cactus soil casing to try to adjust the ph (don't think it was enough tho').

I had blobs forming at day 10 after spawning the shoebox. Actual pins at day 18 I think. So for me it behaved EXACTLY as with the “fast” lineage I already have, you can look up photos from the procedure, same colors, same spaghetti shoebox.

Enough talk, here’s the photo:


Next step will be to trip with these and see how strong they are.

I don’t doubt 0t0lerance for a second on the fact that it’s the “slow” lineage. And actually, a couple of weeks after receiving 0t0lerance’s print I’ve received a print from Konbri, same thing: “slow” lineage. Both 0t0lerance & Konbri have grown it and shared photos and experiences.

I’ve grown Konbri’s print as well – shoebox, same parameters as above - here’s the result 20 days after spawning it (and it behaved exactly as the few lines written above):


The Semperviva/Subtropicalis mystery continues… :shrug:


PS: Since 0t0lerance's print was so big, instead of hoarding it like a dragon, I've cut it in 4 and already sent 2 quarters to 2 other Shroomery members here. A third one will go out this week, so we'll have more trials with this one at some point :pm:






:mindblown:


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OfflineYoshiTrainer
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: chris77] * 3
    #28391415 - 07/10/23 02:53 PM (6 months, 16 days ago)

Nice Melgo, I look forward to your report!


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Invisiblekirkeng
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: YoshiTrainer] * 3
    #28391439 - 07/10/23 03:23 PM (6 months, 16 days ago)

That’s very interesting melgo. I’m sending some of that line to grain this week so hopefully have another report in the coming months.

Do you think it’s coming down to environmental conditions? I also seem to recall someone having a slow pins with the “fast” line sometime back, I could be totally miss rembering that though and getting the lines mixed up in hazy memory. If it is down to environmental conditions I wonder what the catalyst is? Are there any similarities of how the slow genetics are being fruited va the fast ones? I’m wondering if the more pronounced fluctuations of conditions in tubs could be a reason?

I’m just thinking out loud, is there any similarities between fast vs slow compared to substrate composition? Shits got me wondering now. I’m curious to see how the “slow” ones perform for me now even more.


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Offlinepsilocybist
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: kirkeng] * 2
    #28391522 - 07/10/23 04:48 PM (6 months, 16 days ago)

How I wish I had another print, or even a leftover of one, to send to Melgo, so to compare/confirm what SLOW actually is.
This also was from CaptainFuture, from late 2015 or early 2016.

So far it's been 4 months since germinated and expanded, yet it doesn't die, nor does it fruit. It wasn't that slow on grain, but considering the temps (avg 25°C, max 32°C, min 23°C) it wasn't fast.

I'll try a re-casing soon, with a few different materials to have pH and Content of casings tested.


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OfflineMelgo
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: psilocybist] * 2
    #28391998 - 07/11/23 12:54 AM (6 months, 15 days ago)

Quote:

kirkeng said:
That’s very interesting melgo. I’m sending some of that line to grain this week so hopefully have another report in the coming months.

Do you think it’s coming down to environmental conditions? I also seem to recall someone having a slow pins with the “fast” line sometime back, I could be totally miss remembering that though and getting the lines mixed up in hazy memory. If it is down to environmental conditions I wonder what the catalyst is? Are there any similarities of how the slow genetics are being fruited va the fast ones? I’m wondering if the more pronounced fluctuations of conditions in tubs could be a reason?

I’m just thinking out loud, is there any similarities between fast vs slow compared to substrate composition? Shits got me wondering now. I’m curious to see how the “slow” ones perform for me now even more.




Very cool! shruglife told me yesterday he sent it to agar, and I'm sending the 3rd quarter to The Tao this week.

"Do you think it’s coming down to environmental conditions?" It could be the big question! :laugh: Looking forward to what you'll arrive at :yesnod:

Quote:

psilocybist said:
How I wish I had another print, or even a leftover of one, to send to Melgo, so to compare/confirm what SLOW actually is.
This also was from CaptainFuture, from late 2015 or early 2016.

So far it's been 4 months since germinated and expanded, yet it doesn't die, nor does it fruit. It wasn't that slow on grain, but considering the temps (avg 25°C, max 32°C, min 23°C) it wasn't fast.

I'll try a re-casing soon, with a few different materials to have pH and Content of casings tested.




Haha, yeah I see what you mean :wink: The frustration of waiting so long for pins to show up. I was somewhat disappointed when it fruited so fast :shrug:

My average temp right now is 21°C - Semp's loving it.

This week I'll spawn 3 jars of another T1 from Konbri's print. I'll change the spawn/sub ratio to 3/7 and keep everything else the same. Testing if spawn/sub ratio has any influence.

I'm also wondering what you guys do for mycelium selection? Here's my approach:
  • Swab spore print to agar.
  • Select one of the fastest germination, transfer it to an agar plate (so that's T1 for me).
  • Let the T1 mycelium colonize the agar plate close to 100%.
  • Slice the agar plate in 3 wedges, and one wedge per jar - so 3 rye jars.
  • Spawn these 3 rye jars to 1 shoebox.


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Offline0t0lerance
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: Melgo] * 2
    #28392019 - 07/11/23 01:44 AM (6 months, 15 days ago)

Quote:

Melgo said:
I'm also wondering what you guys do for mycelium selection? Here's my approach:
  • Swab spore print to agar.
  • Select one of the fastest germination, transfer it to an agar plate (so that's T1 for me).
  • Let the T1 mycelium colonize the agar plate close to 100%.
  • Slice the agar plate in 3 wedges, and one wedge per jar - so 3 rye jars.
  • Spawn these 3 rye jars to 1 shoebox.





What I do is no selection at all to be honest. I have those re-useable petri's (aliens holy agar). And just touch the centre of each T0.. let's say 6x T0.. then once each plate grows out 3/4 I make a 'triforce' - legend of zelda - shape.. if that rings a bell.. ditch the middle wedge as it harbors bacteria and other contaminants.. then I'm left with 3 outer wedges.. 6x3 = 18x T1 plates.. grow it too 100%, then each T1 plate I move to it's own jar (cut it in 9 pieces us the entire plate and move all wedges to 1 jar)..

To be honest, I haven't seen 1 subtropicalis wedge grow faster than another.. everything seems grows at the same pace..

So I get a wide genetic mix.. but to be honest I haven't been able to produce a good clone yet.. I failed to follow a proper procedure too.. failed to use good coir.. so I messed up the past 6 months, I admit. So what I hope to do with the big tote I have running.. contains 16 jars, so shitload of genetics, I hope to get a few massive fruits with that p9 tek.. and then make clones and do some consistent growing with the tote.


Edited by 0t0lerance (07/11/23 01:51 AM)


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Invisiblekirkeng
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: 0t0lerance] * 2
    #28392095 - 07/11/23 05:40 AM (6 months, 15 days ago)

Yeah I’m not crazy picky with my selection either, most germ plates don’t show any sections that are growing exceptionally faster. Usually sending a small wedge from t1, I take very small transfers. Usually same with a clone too, t1 plates get sent unless they’re dirty of course.

I did just g2g an clone master that was inoculated with a clone that’s been sitting around for like 6-8 months now at room temp, the master grew out much thinner and whisper than usual, I did prep my grains a bit dry that time though so it is either that or the age. We’ll see if they get back to normal after being moved to wetter grains


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OfflineMelgo
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: kirkeng] * 1
    #28392100 - 07/11/23 05:50 AM (6 months, 15 days ago)

Quote:

0t0lerance said:
What I do is no selection at all to be honest. I have those re-useable petri's (aliens holy agar). And just touch the centre of each T0.. let's say 6x T0.. then once each plate grows out 3/4 I make a 'triforce' - legend of zelda - shape.. if that rings a bell.. ditch the middle wedge as it harbors bacteria and other contaminants.. then I'm left with 3 outer wedges.. 6x3 = 18x T1 plates.. grow it too 100%, then each T1 plate I move to it's own jar (cut it in 9 pieces us the entire plate and move all wedges to 1 jar)..

To be honest, I haven't seen 1 subtropicalis wedge grow faster than another.. everything seems grows at the same pace..

So I get a wide genetic mix.. but to be honest I haven't been able to produce a good clone yet.. I failed to follow a proper procedure too.. failed to use good coir.. so I messed up the past 6 months, I admit. So what I hope to do with the big tote I have running.. contains 16 jars, so shitload of genetics, I hope to get a few massive fruits with that p9 tek.. and then make clones and do some consistent growing with the tote.




18 T1 plates! Holy shit, that's a lot! I do 4, max 6. I feel overwhelmed and unable to focus otherwise.

Quote:

kirkeng said:
Yeah I’m not crazy picky with my selection either, most germ plates don’t show any sections that are growing exceptionally faster. Usually sending a small wedge from t1, I take very small transfers. Usually same with a clone too, t1 plates get sent unless they’re dirty of course.

I did just g2g an clone master that was inoculated with a clone that’s been sitting around for like 6-8 months now at room temp, the master grew out much thinner and whisper than usual, I did prep my grains a bit dry that time though so it is either that or the age. We’ll see if they get back to normal after being moved to wetter grains




Same, I don't get plates growing exceptionally faster, but sometimes I see some that grow a bit faster than others so I select these (plus the fact that there were the first to germinate).

Thanks for your input guys, curious to read what the others do.


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Invisiblekirkeng
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: Melgo] * 1
    #28392152 - 07/11/23 07:03 AM (6 months, 15 days ago)

Oh I see, I think I missread your initial post, I’d say I also tend to select what germinates first, not always but a lot of the time. I thought you were referring sections that were growing faster… my bad. I probably only take 1 or 2 t1’s usually only 1. I only have like 100 plates to work with and don’t have room for more usually, still waiting to find a good deal on another case…


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Offline0t0lerance
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: Melgo] * 1
    #28392158 - 07/11/23 07:10 AM (6 months, 15 days ago)

Quote:

Melgo said:
18 T1 plates! Holy shit, that's a lot! I do 4, max 6. I feel overwhelmed and unable to focus otherwise.





Oh, I find it calming and take my time for it. But I admit I prefer max 12 plates.. 18 is too much. As I just need 3 quarts to a brick (6 plates) and ofcourse I want to prevent doing it again.. so I rather make more and toss plates which contaminated.. but they end up clean so I tend to use them as I don't want to waste them haha :grin:.. but then again after sending to bulk all contaminated after some months so nothing to show for it in the end :waitingpatiently:


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InvisibleMojaveMyc
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: 0t0lerance] * 7
    #28392282 - 07/11/23 09:48 AM (6 months, 15 days ago)

I swab a few plates & wait for them to pin. The culture that throws the most plins is the winner. Sometimes I’ll use the plin if it looks dope, but I usually go for the myc it grew from.



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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: MojaveMyc] * 2
    #28392493 - 07/11/23 01:54 PM (6 months, 15 days ago)

Lately I do one plate for each line I'm running. Maybe 2 transfers off that so 2 plates of T1 to grain.

Still waiting on pins in the garden but seeing Psychos gives me hope. They are hiding in cheat grass.
Hindsights and my line are in bags fully colonized for about a month now with no changes. So yeah, we in for another month to two.


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OfflineMelgo
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: rockyfungus] * 3
    #28393057 - 07/12/23 01:11 AM (6 months, 14 days ago)

Quote:

MojaveMyc said:
I swab a few plates & wait for them to pin. The culture that throws the most plins is the winner. Sometimes I’ll use the plin if it looks dope, but I usually go for the myc it grew from.






A lot of people are here struggling for the damn thing to pin once spawned, and MojaveMyc comes here and tells us he just waits for it to pin on AGAR to do his selection. The Universe has a twisted sense of humor :rofl:

Good on you Mate :yesnod::thumbup:

How long do you have to wait for these agar pins? Where did you get your print from? How many grams of dried material do you have to take for a serious trip? So many questions... :naughty:

Quote:

rockyfungus said:
Lately I do one plate for each line I'm running. Maybe 2 transfers off that so 2 plates of T1 to grain.

Still waiting on pins in the garden but seeing Psychos gives me hope. They are hiding in cheat grass.
Hindsights and my line are in bags fully colonized for about a month now with no changes. So yeah, we in for another month to two.




:thumbup:

Do you remember where did you get your print from?


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InvisibleMojaveMyc
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: Melgo] * 5
    #28393262 - 07/12/23 08:37 AM (6 months, 14 days ago)

The universe can certainly be a bit of a prankster :lol: I got that tip from Mycorry’s post in the early days of the thread. I usually see plins after about a month depending on the size of the plate. My agar is grain water + nutritional yeast.

I got mine from Sonoran Spores, no reply when I asked where he got them from. I thought I had slowpokes because my first few tubs took a little over 2 months from spawn-harvest. The only things that have changed since then are the climate I live in & grain of choice.

Most of my trips are on freshies. The time I ate them dry I’d say .6g got me to level 2. Fresh, 9g was a strong level 4. I’m on the sensitive side. Most friends take 1-1.5g, the low sensitivity homie maxes out at 4g. Potency has def varied, with the weakest being on par with Tamps.

I believe we have the same questions Melgo :lol: I am so damn curious about this species & why our collective results vary. At this point it seems that many of us are doing the same thing so it’s gotta be genetics, but your recent grow is challenging that idea. What gives!?


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OfflineAdas
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: Melgo] * 5
    #28393418 - 07/12/23 11:09 AM (6 months, 14 days ago)

Quote:

Melgo said:
Quote:

MojaveMyc said:
I swab a few plates & wait for them to pin. The culture that throws the most plins is the winner. Sometimes I’ll use the plin if it looks dope, but I usually go for the myc it grew from.






A lot of people are here struggling for the damn thing to pin once spawned, and MojaveMyc comes here and tells us he just waits for it to pin on AGAR to do his selection. The Universe has a twisted sense of humor :rofl:





Right? But what gets me the most is the THICCness of these plate pins! Looks like they got a ton of FAE :rofl:


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OfflineMelgo
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: Adas] * 4
    #28394036 - 07/13/23 01:58 AM (6 months, 13 days ago)

Quote:

MojaveMyc said:
The universe can certainly be a bit of a prankster :lol: I got that tip from Mycorry’s post in the early days of the thread. I usually see plins after about a month depending on the size of the plate. My agar is grain water + nutritional yeast.

I got mine from Sonoran Spores, no reply when I asked where he got them from. I thought I had slowpokes because my first few tubs took a little over 2 months from spawn-harvest. The only things that have changed since then are the climate I live in & grain of choice.

Most of my trips are on freshies. The time I ate them dry I’d say .6g got me to level 2. Fresh, 9g was a strong level 4. I’m on the sensitive side. Most friends take 1-1.5g, the low sensitivity homie maxes out at 4g. Potency has def varied, with the weakest being on par with Tamps.

I believe we have the same questions Melgo :lol: I am so damn curious about this species & why our collective results vary. At this point it seems that many of us are doing the same thing so it’s gotta be genetics, but your recent grow is challenging that idea. What gives!?




Oh, thanks for the details "My agar is grain water + nutritional yeast." - I'll have to test that at some point, see if it is a factor for agar pins.

With these info, yeah we'd suppose you have the "slow" genetics. I have another print from Konbri, this lineage came from Angam who got it from a vendor. I'll try to revive this one in a distilled water solution this week-end, and have another go at some "slow" lineage. And I intend to trip from my last harvest (in the dryer atm) in 2 weeks. Will see about dosage.

This species is so interesting :yesnod:

Quote:

Adas said:
Right? But what gets me the most is the THICCness of these plate pins! Looks like they got a ton of FAE :rofl:




Bro! Yeah, THAT too!!! It's mental :lol:


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