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0t0lerance


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Re: Official Hoogshagenii/Semperviva Thread [Re: RoscoeReturns] 7
#28387838 - 07/07/23 12:52 AM (6 months, 19 days ago) |
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It's something.. poor performing subtrop clone that decided to fruit after 2 months. A well, got to share failures too right? The bag right next to it contains zap 'la martinica'.. it's has been doing nothing for 4 months now. Thinking about tossing it.. or let it ride until contamination claims it.
Currently looking more into medicinal species (reishi, cordyceps, turkey tail) very busy with that, so I'll be less active with actives, but I'll keep you guys posted about the 120 quart subtrop tote.
Edited by 0t0lerance (07/07/23 12:55 AM)
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psilocybist
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Re: Official Hoogshagenii/Semperviva Thread [Re: 0t0lerance]
#28388236 - 07/07/23 10:14 AM (6 months, 19 days ago) |
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Just to sympathize with myself, it's day 61 since I spawned to bulk. Still no pin, no contam, no nothing.
Do you think it's good idea to submerge the sub (which are in buckets, each around 0.5 kg) in water, just like cakes? They haven't dried up, but the surface is not glistening in humidity/dampness anymore.
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Konbri



Registered: 10/16/17
Posts: 141
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: psilocybist]
#28388471 - 07/07/23 02:26 PM (6 months, 19 days ago) |
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For some reason even after dunking with such long waiting periods I get most pins at the rim, even with pools of water and damp surface. If I were you and was really assured that my cactus casing was fine and dandy, I would case it. Two of my cakes in same circumstances were cased a couple weeks ago and still no pins... Those which pinned within a month are fruiting vigorously.
I wonder how is Melgo's slow genetics doing
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psilocybist
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: Konbri]
#28388478 - 07/07/23 02:41 PM (6 months, 19 days ago) |
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They're actually all cased, with coco+verm mix. It's at least a month after casing.
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Melgo
Semperviva Fanatic



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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: Konbri] 2
#28388508 - 07/07/23 03:18 PM (6 months, 19 days ago) |
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Quote:
Konbri said: I wonder how is Melgo's slow genetics doing 
Y'all talking about "slow" genetics, I ain't seeing no slow genetics 

Joking aside, I was waiting to have proper pins to send you a message. But the shoebox from the print you sent me, showed the first blobs 10 days after spawning it 
A week later (today) they're huge and I predict a massive pinset. Don't know how big these fruits will be, could be tiny fruits like from the print 0t0lerance sent me (you can check it out in my journal).
For the last 3 days, I've been taking a photo of the shoebox from the print Konbri sent me. I intend to do so everyday to create a GIF at the end of the grow. Just for fun really, hopefully I can share it here.
So yeah, I don't know about the slow genetic part yet. Is there something right that I'm using/doing/lucking out
Edited by Melgo (07/07/23 11:22 PM)
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Konbri



Registered: 10/16/17
Posts: 141
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: Melgo] 2
#28388627 - 07/07/23 05:18 PM (6 months, 19 days ago) |
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This mushroom is a total mystery to me. I'll be laughing so hard if "quick" lineage print takes forever to pin for me. If the letter reaches me at all... Is there anyone who has both slow and quick genetics and nailed them? So far one could get an impression that it's not genetics issue, but something else, something mysterious...
Still, I have so much range of pinning times and fruit sizes! I would think it's something wrong in my technique or spawn, if not for others with similar problems. I plan to spawn to untreated manure next weekend, I really want to tune a method that works. Waiting three weeks is one thing, three months spawn to PIN is really too long a time, a whole season, and the fruits are sparse, even if pretty large. I would try manure long ago, but it's only now I found a supplier who has aged horse manure, not some pellets of unknown quality.
2 psilocybist - yes, I meant I would try cactus soil if I had it, even on top of older casing. Not encouraging, just thinking aloud. With my particular verm (I have only one local source) results are not too nice, fruits are short and much darker with smaller caps, pin later if at all.
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RoscoeReturns
Crotchety chode man



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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: Konbri] 3
#28388724 - 07/07/23 07:16 PM (6 months, 19 days ago) |
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Are you still waiting for the print I sent? If so, shoot me your info again and I’ll mail more next time I print them. Got a few bags pinning now.
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Bigworm



Registered: 03/19/22
Posts: 1,873
Loc: Murica
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: RoscoeReturns] 1
#28388836 - 07/07/23 09:13 PM (6 months, 19 days ago) |
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This is a clone that I sent to bulk on 5/30. These are the only 2 mushrooms that popped up. The clone was from a dense cluster and I chose one from the center of it. I guess I chose wrong. Cased with pasteurized verm and sand 2 weeks after spawn.
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psilocybist
All is One



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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: Bigworm] 1
#28388942 - 07/08/23 02:18 AM (6 months, 18 days ago) |
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Though haven't grown a successful flush yet, but I might have a theory. I think this species "requires" some other microorganisms to pin. So I guess, not just humidity/temp factors play a role in pinning, but also some bacteria. Saying this cuz I saw one of my grain bags, (when inspecting before spawning to bulk, around two months ago) had a zone of dense dense dense aborted pins. And it didn't smell well, so I dumped it into trashcan. It was not exposed to light, the pins were on the outer surface of bag, and inside the garins (interior parts) some grayish/black mold was grown.
I know it's not new to us to see mycelium pinning in reaction to contams, but considering that all my other bags (which came from antibiotic agar and LC media) were %100 clean of bacteria, and none of my buckets so far are pinning neither contammed, kinda validates the idea. To remind, the contammed bag pinned 3 weeks after inoculation by LC, while it's two months and the buckets are not pinning.
Maybe there's some beneficiary bacteria specimen which happens to be there in spawn/subs, for some of us, and absent for the rest of us. Or maybe the psuedo-clean casings happen to provide some of those microorganisms to the mycelium to trigger the pinning.
This explains why pinning is such different/difficult for some of us while for some it just happens normaly. And why clones don't act as expected. Maybe the unseen/overlooked factor is some kind of invisible/nondestructive microbe (most probably some soil-inhabiting bacteria) that co-operates with the mycelium.
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Adas
Lonely Dreamer



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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: Konbri] 3
#28388970 - 07/08/23 04:03 AM (6 months, 18 days ago) |
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Quote:
Konbri said: This mushroom is a total mystery to me. I'll be laughing so hard if "quick" lineage print takes forever to pin for me. If the letter reaches me at all... Is there anyone who has both slow and quick genetics and nailed them? So far one could get an impression that it's not genetics issue, but something else, something mysterious...
Still, I have so much range of pinning times and fruit sizes! I would think it's something wrong in my technique or spawn, if not for others with similar problems. I plan to spawn to untreated manure next weekend, I really want to tune a method that works. Waiting three weeks is one thing, three months spawn to PIN is really too long a time, a whole season, and the fruits are sparse, even if pretty large. I would try manure long ago, but it's only now I found a supplier who has aged horse manure, not some pellets of unknown quality.
2 psilocybist - yes, I meant I would try cactus soil if I had it, even on top of older casing. Not encouraging, just thinking aloud. With my particular verm (I have only one local source) results are not too nice, fruits are short and much darker with smaller caps, pin later if at all.
Wouldn't say I've "nailed" them, but I've grown both with success.
Psilocybist, I definitely don't think they need any special microbes to pin. People have posted invitro pins before, and I've had plate pins too.
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SirPsycho
Purple Belt in Google-Fu



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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: Adas] 9
#28388997 - 07/08/23 05:40 AM (6 months, 18 days ago) |
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Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints Balance in life is like running on ice.
🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖
    "Mist your balls and fan your asshole" - Pandaskis, 2023
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A.k.a
Stranger



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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: SirPsycho] 1
#28389097 - 07/08/23 08:12 AM (6 months, 18 days ago) |
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Pinning from a bag that smelled off was probably an emergency response trying to get spores off asap
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LAGM2020     
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psilocybist
All is One



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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: A.k.a]
#28389153 - 07/08/23 09:17 AM (6 months, 18 days ago) |
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I just don't understand why my buckets haven't pinned yet, considering the conditions.
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SirPsycho
Purple Belt in Google-Fu



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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: psilocybist] 2
#28389295 - 07/08/23 12:10 PM (6 months, 18 days ago) |
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My discussion with Mycolorado over in the stone producers thread got me wondering. Has Alan Rockefeller or anyone sequenced subtropicalis?
Reason for question, subtrop and tampanesis:
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Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints Balance in life is like running on ice.
🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖
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Konbri



Registered: 10/16/17
Posts: 141
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: SirPsycho]
#28389514 - 07/08/23 04:24 PM (6 months, 18 days ago) |
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I thought he did it, and that's the reason why we call it subtropicalis. I thought it was Alan who proved that hoogshagenii var.convexa, semperviva and subtrops are the same Psilocybe, but maybe my memory is just wandering and creating false memories lol.
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shruglife
¯\_(ツ)_/¯
Registered: 10/22/20
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: Konbri] 1
#28390191 - 07/09/23 10:10 AM (6 months, 17 days ago) |
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Today I learned that fully colonized semp grain spawn doesn't respond well to being put in the fridge! I do this with cubes if I know that it'll be a while before I send a batch, and it's always just fine. This semp grain heavily bruised and pissed metabolites like crazy. Since the bruising isn't consistent between jars I'm curious if it might be an indicator of slight contamination.
I guess we'll see how it goes from here ¯\_(ツ)_/¯
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Konbri



Registered: 10/16/17
Posts: 141
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: shruglife]
#28390415 - 07/09/23 02:40 PM (6 months, 17 days ago) |
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I've never stored jars in the fridge, is there a consensus on why one shouldn't store spawn at room temp? I've done so a lot of times, because I never get in vitro spawn pins (I keep wondering why), and usually it's fine for a few months, but 6+ months usually show lower yields and a tiny bit slower bulk colonization time, both natalensis and cubes. Mexicana works awesome at long ranged room temp, and semperviva is hard to say, because I can't nail the technique and inconsistent result has a lot of vectors to consider.
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SirPsycho
Purple Belt in Google-Fu



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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: Konbri] 10
#28390489 - 07/09/23 03:33 PM (6 months, 17 days ago) |
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I once grew a semp this big
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Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints Balance in life is like running on ice.
🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖
    "Mist your balls and fan your asshole" - Pandaskis, 2023
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YoshiTrainer
Onion tied to belt



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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: SirPsycho] 3
#28390563 - 07/09/23 04:48 PM (6 months, 17 days ago) |
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Those are F/N awesome SirP!
Subtropicalis grains can be stored at room temps for many months. Subtrop mycelium is active, more so after it sits. I recommend everyone start a jar of subtrop mycelium tek along with jars of spawn.
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Melgo
Semperviva Fanatic



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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: SirPsycho] 3
#28390925 - 07/09/23 11:21 PM (6 months, 16 days ago) |
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