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psilocybist
All is One



Registered: 05/22/14
Posts: 528
Loc: Far
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: rockyfungus] 2
#28354522 - 06/10/23 01:37 PM (7 months, 15 days ago) |
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Thanks for sharing your experiences about this specific issue. So It's not an issue actually. I just didn't know the slow lineage semps are such slow, and was assuming/anticipating a max duration of 3 months spore-to-spore. Seems times around twice as long is common. So I'll wait and wait and wait.
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RoscoeReturns
Crotchety chode man



Registered: 02/12/18
Posts: 1,738
Loc: State of Confusion
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: psilocybist] 7
#28354753 - 06/10/23 06:07 PM (7 months, 15 days ago) |
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When my bags throw up one or two early mushrooms, they get big!

When they push up in clusters they stay much smaller.
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Fungus Gnat


Registered: 11/08/22
Posts: 196
Loc: Oregon
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: RoscoeReturns] 7
#28354785 - 06/10/23 06:37 PM (7 months, 15 days ago) |
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Quote:
RoscoeReturns said:

Those are monsters!
1 month from spawning, still watching them noodle around. I think I should have given them a proper overhead light source.
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Melgo
Semperviva Fanatic



Registered: 02/11/16
Posts: 692
Loc: Western Europe
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: Fungus Gnat] 2
#28355176 - 06/11/23 03:50 AM (7 months, 14 days ago) |
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I have a shoebox of the slow lineage spawned to CV, fully colonized and cased with cactus soil. I'll follow chris77 and shruglife's advice on restricting FAE until seeing the first pins.
And now:
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chris77
Archaic Revivalist



Registered: 01/17/22
Posts: 1,868
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: Fungus Gnat] 2
#28355214 - 06/11/23 05:22 AM (7 months, 14 days ago) |
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Quote:
kingev said:
1 month from spawning, still watching them noodle around. I think I should have given them a proper overhead light source.


delicious noodle salad indeed
-------------------- the observer is the observed j. krishnamurti
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MojaveMyc
Fractal Farmer



Registered: 05/08/21
Posts: 656
Loc: Colorado
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: chris77] 19
#28358073 - 06/13/23 08:11 AM (7 months, 12 days ago) |
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Day 30, 20 days from pins to harvest. 1:2, no casing or misting. 506g wet
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0t0lerance


Registered: 05/01/21
Posts: 962
Loc: The Netherlands
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: MojaveMyc] 1
#28358083 - 06/13/23 08:25 AM (7 months, 12 days ago) |
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Quote:
MojaveMyc said:
 
Day 30, 20 days from pins to harvest. 1:2, no casing or misting. 506g wet 
Very nice, congratulations!!
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chris77
Archaic Revivalist



Registered: 01/17/22
Posts: 1,868
Last seen: 6 hours, 47 minutes
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: MojaveMyc] 1
#28358085 - 06/13/23 08:27 AM (7 months, 12 days ago) |
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Quote:
MojaveMyc said:
 
Day 30, 20 days from pins to harvest. 1:2, no casing or misting. 506g wet 
thats fantastic
-------------------- the observer is the observed j. krishnamurti
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MojaveMyc
Fractal Farmer



Registered: 05/08/21
Posts: 656
Loc: Colorado
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: chris77]
#28358115 - 06/13/23 08:58 AM (7 months, 12 days ago) |
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Thanks homies! pretty jazzed about this one
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YoshiTrainer
Onion tied to belt



Registered: 04/30/22
Posts: 1,204
Loc: Castles made of sand
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: MojaveMyc]
#28358163 - 06/13/23 09:33 AM (7 months, 12 days ago) |
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That is quite a tub Mojave, great job!
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psilocybist
All is One



Registered: 05/22/14
Posts: 528
Loc: Far
Last seen: 18 hours, 50 minutes
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: MojaveMyc] 1
#28358427 - 06/13/23 01:09 PM (7 months, 12 days ago) |
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Quote:
MojaveMyc said:
 
Day 30, 20 days from pins to harvest. 1:2, no casing or misting. 506g wet 
Niceeeeeeee, how many days from spawn to pin? And what's the sub mix?
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RoscoeReturns
Crotchety chode man



Registered: 02/12/18
Posts: 1,738
Loc: State of Confusion
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: MojaveMyc] 1
#28358829 - 06/13/23 07:06 PM (7 months, 12 days ago) |
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Quote:
MojaveMyc said:
 
Day 30, 20 days from pins to harvest. 1:2, no casing or misting. 506g wet 
Beautiful!
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MojaveMyc
Fractal Farmer



Registered: 05/08/21
Posts: 656
Loc: Colorado
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: RoscoeReturns] 4
#28359262 - 06/14/23 06:55 AM (7 months, 11 days ago) |
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Thank you for the kind words guys They dried down to 59g, my best first flush from any species. I agree with the sentiment that it's only a matter of time until these gain popularity.
@ Psilocybist I spotted the first pins on day 10 & the sub mix is just coir/verm. I'm not sure if it has made a difference but this is my first round of grows after switching from wheat to millet.
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ekabpils
Exploring Exotics



Registered: 10/20/21
Posts: 167
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Re: Official Hoogshagenii/Semperviva Thread [Re: RoscoeReturns] 11
#28359268 - 06/14/23 07:06 AM (7 months, 11 days ago) |
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My first one grow of Hoogshageni:
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chris77
Archaic Revivalist



Registered: 01/17/22
Posts: 1,868
Last seen: 6 hours, 47 minutes
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Re: Official Hoogshagenii/Semperviva Thread [Re: ekabpils] 5
#28359316 - 06/14/23 08:30 AM (7 months, 11 days ago) |
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Hello, before i put them in the marketplace tomorrow, i have 2 semperviva prints to give away. pm me if you are interested they are supposedly from the slow lineage (although there seem to be much slower ones out there) chris
edit: all prints gone.
-------------------- the observer is the observed j. krishnamurti
Edited by chris77 (06/15/23 05:00 AM)
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Smoothcat
Renegade-master



Registered: 10/07/15
Posts: 1,254
Last seen: 5 months, 27 days
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Re: Official Hoogshagenii/Semperviva Thread [Re: chris77]
#28359786 - 06/14/23 03:46 PM (7 months, 11 days ago) |
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Quote:
chris77 said: Hello, before i put them in the marketplace tomorrow, i have 4 senperviva prints to give away. pm me if you are interested they are supposedly from the slow lineage (although there seem to be much slower ones out there) chris
Simply couldn't revive my old print - just seemed to be streaking green 
'd ya
-------------------- Back once again with the ill behaviour Links I like
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ekabpils
Exploring Exotics



Registered: 10/20/21
Posts: 167
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: MojaveMyc]
#28360461 - 06/15/23 05:59 AM (7 months, 10 days ago) |
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Quote:
MojaveMyc said:
 
Day 30, 20 days from pins to harvest. 1:2, no casing or misting. 506g wet 
Hi! What kind of substrate you use here?
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JW123
Nooby Nooby Noob


Registered: 01/17/22
Posts: 1,305
Loc: Tn
Last seen: 3 hours, 49 minutes
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: ekabpils]
#28360683 - 06/15/23 10:58 AM (7 months, 10 days ago) |
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Quote:
MojaveMyc said: @ Psilocybist I spotted the first pins on day 10 & the sub mix is just coir/verm. I'm not sure if it has made a difference but this is my first round of grows after switching from wheat to millet.
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Fungus Gnat


Registered: 11/08/22
Posts: 196
Loc: Oregon
Last seen: 35 minutes, 35 seconds
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: JW123] 8
#28361572 - 06/15/23 10:19 PM (7 months, 10 days ago) |
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My first subtrop harvest from a little takeout container. 1:3 pasteurized under-hydrated coir, spawned 5/10, cased 5/18. Probably could have let them go a little longer but some were dropping spores and I got excited.
 I also ate 1.5g wet while picking them. An hour in I feel relaxed, but not much else noticeable. It ain't no MojaveMyc harvest, but it's honest work! Edit: 2 hours in I definitely felt a bit if a "baby" trip peak. No visual changes, but I have that body feel of a trip and feel a little stoned.
Edited by Fungus Gnat (06/15/23 11:39 PM)
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clampconnected
Researcher



Registered: 04/07/20
Posts: 32
Loc: Portland Oregon
Last seen: 5 days, 5 hours
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: kirkeng] 6
#28361645 - 06/16/23 01:11 AM (7 months, 9 days ago) |
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Quote:
kirkeng said: Recently sent some semperviva to a lab to test for beta carboline presence. Selected fruits that had strong flourescence. Results came in came in negative, no presence, or perhaps too low to identify. These samples may be sent to another lab for confirmation but that is out of my hands. I only received word from the technician so no “proof” so to speak so either take my word or don’t. When I have time I may send in mycelium samples as well considering the paper that detected beta carbolines detected trace amounts in mycelium not fruits. Also when I have time sending in other samples that flouresce namely BVI and weza pan cyans.
Regardless I think it was an interesting result and should definitely be repeated so as to figure out what is causing the flourescence, Whatever it may be. Though I’m wondering if it could be some form of environmental response adaptation taken from the origin locality. Considering I have multiple panaeolus cyanescens from differing localities of which only some flouresce, so it being some intrinsic genetic thing I would expect every locality to flouresce given all the same species. But hey I am but a mere grower and nothing more so I can’t really speak to anything outside of that. But there is some mystery there perhaps someone more knowledgeable than I can shed some light.
Edit: I forgot to add significant increase in overall potency was also detected in comparisons between harvests two weeks from pins/primordia to 4 weeks from pins/primordia. Bear in mind those harvest periods are estimates as I wasn’t necessarily counting days so expect a plus or minus a few days on both those values. On the scale of 7-8mg/g increase in the longer maturation period. That clears up any debate on whether waiting is better as well, though we all already expected that one. Also for clarification this is not a comparison between slow vs fast genetics. These tests were done with the fast genetics and is only a representation of ideal harvest times for specific genetics. Also if anyone wants to donate me some slow genetics I’ll eventually get around to sending those out for testing as well! Can’t say when but eventually.
I’ve been working on looking for b carbolines with my hplc uv, it’s not easy as even extremely small quantities can fluoresce extremely bright. I’ve got a decent process down to extract and concentrate them, now I’m going to try for a larger extraction and see if I can get enough to show up on the machine. Alternatively, tlc can be used to at least visualize them. Still working on getting some of the carbolines resolved from each other on tlc. Subtrop is interesting, I’ve got two different strains in LC here, ‘Coyopolan’ and ‘Xico’, both wild clones, and they express quite different fluorescence on the same LC recipe. Maybe some difference in their culture conditions before inoculating to LC caused this differential expression, or maybe the two strain chemo types are different.
Extracts are 3x meoh, evaporated, crude residue taken up in small amount of meoh, insoluble residue taken up in ph 12 h2o. Basic h2o extracted with ethyl acetate 3x, organic fraction evaporated, then taken up in meoh for analysis. Meoh soluble used to see tryptamines, ethyl acetate fraction of basic wash used to look for carbolines.
Also, don’t forget about lumichrome, it is the most concentrated fluorescent compound in Psilocybe mushrooms, as far as we know.
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