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Invisiblekirkeng
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: psilocybist]
    #28336902 - 05/27/23 12:19 PM (7 months, 29 days ago)

I may give that a try, I did try a extraction on a 50g dry sample some time back utilizing the same method I’ve used for Syrian rue extracts and didn’t pull anything from the extract. Granted I do not claim to be any sort of chemistry person so can’t say much beyond that, or even if that method is suitable for mushrooms. Though the tests were done via hplc so I’d expect good accuracy in their result. If they get sent to another lab perhaps more knowledge can be gained. But as always this is just one point in the graph so nothing is certain obviously, but hey at least we got some semblance of data started.


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OfflineAdas
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: MojaveMyc] * 1
    #28337003 - 05/27/23 01:32 PM (7 months, 29 days ago)

Quote:

MojaveMyc said:

Day 39 on this one. Not the prettiest :lol: more pins slowly coming in. It likely could've benefited from a casing layer. 1:4


Day 13, spotted first pins on day 10. 1:2

The fast ones give me full flushes. Does anyone else notice that slower cultures tend to fruit sporadically, a few at a time? Otherwise I'm thinking that I'm failing to maintain surface conditions during the extended wait. Thanks for the future feedback homies




I feel like they definitely get more beautiful if you give them a casing layer.

Kirk, awesome work! Thanks for this knowledge. So the exploration continues. It's shocking to me that some Pan Cyans show fluorescence, it's the first time I hear this. If I could give you another +5, I would! :rockon:


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Invisiblekirkeng
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: Adas] * 1
    #28337098 - 05/27/23 02:30 PM (7 months, 29 days ago)

Thanks Adas! Yeah the pans took me by surprise. Once I saw the flourescence in semps I tried everything, so far from least to most flourescence I’ve observed: mexicana, pan cyan weza, semperviva, and BVI. Honestly at this point the pans are most interesting to me. I’ll also comparing the flourescence of these mushrooms and their water extract counterparts the color of the flourescence is different than that of hydrated rue or caapi. Similar color but almost darker. I’m thinking of diluting some rue or caapi tea and comparing that color to see if it more closely resembles the mushroom tea/fruits.

Got me wondering as well if it is not beta carbolines then could it be having this flourescence for some purpose to do with insects? I seem to recall them being able to see uv spectrum. I dunno it has wracked more questions in my head which I suppose I prefer since it keeps the magic alive.


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OfflineAdas
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: kirkeng] * 1
    #28337101 - 05/27/23 02:33 PM (7 months, 29 days ago)

There are almost definitely no MAOIs in Subtrops, since my strong trip only lasted like 2 hours lol. There are confirmed ß-Carbolines in Tamps, and boy do the trips actually last long. After you pass the 2g mark, that is.


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Invisiblekirkeng
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: Adas] * 1
    #28337104 - 05/27/23 02:39 PM (7 months, 29 days ago)

Fair, I do recall my larger ramp trips lasting quite some time, granted my semp ones do as well for me. Very curious all around. If I recall was it detected in fruits or stones? I remember that one paper saying stones and mycelium but can’t remember if they did fruits as well. Eventually I’d love to find a mushroom or mycelium with substantial quantity for use in some changa blends. Perhaps someday.


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OfflineAdas
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: kirkeng] * 1
    #28337105 - 05/27/23 02:44 PM (7 months, 29 days ago)

I believe it was in fruits. The compounds in question were Cordysinins of some kind.


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Invisibleshruglife
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: kirkeng] * 1
    #28337149 - 05/27/23 03:41 PM (7 months, 29 days ago)

Quote:

kirkeng said:
Recently sent some semperviva to a lab to test for beta carboline presence. Selected fruits that had strong flourescence. Results came in came in negative, no presence, or perhaps too low to identify. These samples may be sent to another lab for confirmation but that is out of my hands. I only received word from the technician so no “proof” so to speak so either take my word or don’t. When I have time I may send in mycelium samples as well considering the paper that detected beta carbolines detected trace amounts in mycelium not fruits. Also when I have time sending in other samples that flouresce namely BVI and weza pan cyans.

Regardless I think it was an interesting result and should definitely be repeated so as to figure out what is causing the flourescence, Whatever it may be. Though I’m wondering if it could be some form of environmental response adaptation taken from the origin locality. Considering I have multiple panaeolus cyanescens from differing localities of which only some flouresce, so it being some intrinsic genetic thing I would expect every locality to flouresce given all the same species. But hey I am but a mere grower and nothing more so I can’t really speak to anything outside of that. But there is some mystery there perhaps someone more knowledgeable than I can shed some light.

Edit: I forgot to add significant increase in overall potency was also detected in comparisons between harvests two weeks from pins/primordia to 4 weeks from pins/primordia. Bear in mind those harvest periods are estimates as I wasn’t necessarily counting days so expect a plus or minus a few days on both those values. On the scale of 7-8mg/g increase in the longer maturation period. That clears up any debate on whether waiting is better as well, though we all already expected that one. Also for clarification this is not a comparison between slow vs fast genetics. These tests were done with the fast genetics and is only a representation of ideal harvest times for specific genetics. Also if anyone wants to donate me some slow genetics I’ll eventually get around to sending those out for testing as well! Can’t say when but eventually.




This is great info, thanks for sending in samples and posting.

Do you have the results for psilocybin and psilocin content that you can share?


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Invisiblekirkeng
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: shruglife] * 6
    #28337171 - 05/27/23 04:03 PM (7 months, 29 days ago)

Yeah, I won’t share the actual document as personal information is in there. But 2 week harvest netted about 17mg psilocybin and 1mg psilicin, 4 week was 23.5mg psilocybin and 1.5mg psilicin.


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OfflineFungus Gnat
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: Adas] * 9
    #28337186 - 05/27/23 04:14 PM (7 months, 29 days ago)

Pins!

:awedance:
Now the real waiting game starts...


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Offlinerockyfungus
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: Adas] * 1
    #28337222 - 05/27/23 04:54 PM (7 months, 29 days ago)

Quote:

Adas said:
There are almost definitely no MAOIs in Subtrops, since my strong trip only lasted like 2 hours lol. There are confirmed ß-Carbolines in Tamps, and boy do the trips actually last long. After you pass the 2g mark, that is.




Interesting. I feel they last longer then pans or cubes for me. Last trip was over 8 hours at 2.5g.


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Offline0t0lerance
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: rockyfungus] * 1
    #28337713 - 05/28/23 01:10 AM (7 months, 28 days ago)

Tamps are still on my to-do list, would like to try them firsthand and see what it does for me. Subtropicalis lasts a short time for me too.. but, I just tried a high dose once, low dosis wear off in 1,5 - 2 hours (so I'm looking for a mushroom with a longer duration)


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Offlineodissey
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: 0t0lerance] * 3
    #28337871 - 05/28/23 06:21 AM (7 months, 28 days ago)

Quote:

0t0lerance said:
Tamps are still on my to-do list, would like to try them firsthand and see what it does for me. Subtropicalis lasts a short time for me too.. but, I just tried a high dose once, low dosis wear off in 1,5 - 2 hours (so I'm looking for a mushroom with a longer duration)



You can always redose before the peak for a longer experience.

My slow genetics gave me prettty long trips while the fast were a 3 hour trip for me


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InvisibleElvishKlingonSith
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: odissey] * 10
    #28338267 - 05/28/23 01:12 PM (7 months, 28 days ago)

Trying to get this across the finish line…



About dead center two fruits are showing a black ring around the caps. Happened overnight. Anything to be concerned about?


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Offlinebambus
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: ElvishKlingonSith]
    #28338668 - 05/28/23 08:07 PM (7 months, 28 days ago)

Quote:

ElvishKlingonSith said:
Trying to get this across the finish line…



About dead center two fruits are showing a black ring around the caps. Happened overnight. Anything to be concerned about?




It looks like they made it across the finish line, you can see some are sporulating. I took the blue rings on mine as a sign that they were done.
Nice job, congrats!


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InvisibleElvishKlingonSith
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: bambus] * 9
    #28339492 - 05/29/23 01:42 PM (7 months, 27 days ago)

Today is a good day.

Special shout-out to the person who knocked up my jars with their LC.  Mad respect.








234g and 170g

Cross posted.

EDIT: 41g dry


Edited by ElvishKlingonSith (05/30/23 01:50 PM)


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OfflineRoscoeReturnsS
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: ElvishKlingonSith] * 2
    #28339497 - 05/29/23 01:44 PM (7 months, 27 days ago)

Nice job!


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InvisibleMojaveMyc
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: RoscoeReturns] * 1
    #28339505 - 05/29/23 01:51 PM (7 months, 27 days ago)

Noice! S/o to that person indeed :bow2:


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OfflineYoshiTrainer
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: MojaveMyc] * 1
    #28339523 - 05/29/23 02:02 PM (7 months, 27 days ago)

Nice harvest! Were they in tubs or a tent? Did you case?


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InvisibleElvishKlingonSith
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: YoshiTrainer] * 2
    #28339533 - 05/29/23 02:13 PM (7 months, 27 days ago)

Quote:

YoshiTrainer said:
Nice harvest! Were they in tubs or a tent? Did you case?




6qt shoebox (no lid) in a modified 20qt gasket box as FC.  As soon as I cased I rested one side of the FC lid on top of its latch to allow for more air.

Birthed shoes on 4/12 at 1:2, 100% coir slightly below field capacity. Cased on 5/3 with 100% verm once I saw knotting.

Misted once or twice a day for 3 weeks (once cased), about a week ago this second pinset really started to take off and I upped the misting to 4+ times per day.


Edited by ElvishKlingonSith (05/29/23 02:28 PM)


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OfflineMelgo
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: kirkeng] * 1
    #28339536 - 05/29/23 02:18 PM (7 months, 27 days ago)

Quote:

kirkeng said:
Yeah, I won’t share the actual document as personal information is in there. But 2 week harvest netted about 17mg psilocybin and 1mg psilocin, 4 week was 23.5mg psilocybin and 1.5mg psilocin.




Oh Wow! That info is priceless! Thanks for sharing it.

Just to be clear: these measurements of psilocybin & psilicin are for how many grams of dried Semperviva?


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