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OfflineAdas
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: odissey]
    #28321675 - 05/16/23 08:43 AM (8 months, 9 days ago)

Thanks for the responses guys! I'm mostly interested in sending dried agar pieces since it's way more stealthy. Same level as prints.

Quote:

odissey said:
Oh on that topic, I took both you tamps and bisporus to fruiting but the bisporus ended up contaming and the tamps never fruited. I still have colonized jars and lc so I will try a jcm instead soon. The prints were pristine though, the fastest clean germinating spores I have had in a long time :dancingshroom:

So what about sending non dried water agar? Seems to work for me. although some get squished, they are still recoverable




Glad the spores worked for you! I suggest you try again. If the Tamps dont fruit try something differently or start again from spores. The Bisporus spores should be still fine too, but of course sooner is better.


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Offlinepsilocybist
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: psilocybist] * 2
    #28321851 - 05/16/23 11:10 AM (8 months, 9 days ago)

Quote:

psilocybist said:
I have mycelium regrowing from dried agar plate, which was going to throw away.
Just transferred a wedge from it to a new plate, mycelium faced down.
Its growth started around 2-4 days after transfer. Didn't check it often.
Species is semperviva. Agar plate was dried for at least 2 months. Will snap some pics tomorrow,  late night now.





This is the plate.
Its growth is very slow, unlike the donor plate before drying. This transfer was done @04/21 (around 25 days ago)
Seems the drying has changed the mycelium behavior. At least grow rate. But it's just theory, as the media recipe is changed as well.


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Offlinechris77
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: psilocybist] * 12
    #28321868 - 05/16/23 11:25 AM (8 months, 9 days ago)



what a special mushroom, color, smell, behaviour... i have generously misted them from time to time. still... curling up as curly as can curl


--------------------
the observer is the observed
            j. krishnamurti


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OnlineFungus Gnat
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: Adas] * 3
    #28321944 - 05/16/23 12:38 PM (8 months, 9 days ago)

I've got to share the bruising/coloration on this PF TEK subtrop jar I've got, inoculated 3/19. I wish I had a UV light!


I sent a couple grain jars from a few posts back on 5/10, 1:3 pasteurized coir in takeout containers. They're looking close to fully colonized, planning on casing this weekend!


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InvisibleaPurpleCray0n
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: chris77] * 1
    #28321966 - 05/16/23 12:57 PM (8 months, 9 days ago)

Quote:

chris77 said:


what a special mushroom, color, smell, behaviour... i have generously misted them from time to time. still... curling up as curly as can curl



I’m groovin on those twisted stipes


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OfflineAdas
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: Fungus Gnat] * 1
    #28322055 - 05/16/23 02:29 PM (8 months, 9 days ago)

Quote:

psilocybist said:
Quote:

psilocybist said:
I have mycelium regrowing from dried agar plate, which was going to throw away.
Just transferred a wedge from it to a new plate, mycelium faced down.
Its growth started around 2-4 days after transfer. Didn't check it often.
Species is semperviva. Agar plate was dried for at least 2 months. Will snap some pics tomorrow,  late night now.





This is the plate.
Its growth is very slow, unlike the donor plate before drying. This transfer was done @04/21 (around 25 days ago)
Seems the drying has changed the mycelium behavior. At least grow rate. But it's just theory, as the media recipe is changed as well.




Try placing it on the old medium next to see what happens.

Quote:

kingev said:
I've got to share the bruising/coloration on this PF TEK subtrop jar I've got, inoculated 3/19. I wish I had a UV light!


I sent a couple grain jars from a few posts back on 5/10, 1:3 pasteurized coir in takeout containers. They're looking close to fully colonized, planning on casing this weekend!





Im pretty sure I see an angel there :lol::angel:


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OnlineFungus Gnat
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: Adas]
    #28322077 - 05/16/23 02:49 PM (8 months, 9 days ago)

I saw wings too! I'd say that's a good omen :grin:


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Offlinephlanx
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: chris77] * 1
    #28322187 - 05/16/23 04:35 PM (8 months, 9 days ago)

Quote:

chris77 said:






That is an awesome picture.  The twisted stipes are gorgeous.

-Phlanx


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InvisibleKonbri
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: nosf3r4tu] * 4
    #28322918 - 05/17/23 08:07 AM (8 months, 8 days ago)

The "long ass genetics". One jar of millet spawn was mixed with coir in a bag and then dumped in the small boxes. At 100% one was cased with peat, the other two with verm. The jar was g2g from another ms jar. I didn't follow the colonization and pinning times for this experiment, but we can see that peat worked better. It worked in another totes as well, much quicker than coir at spawn/coir casing at 100%, which take them 2-3 months from spawn to fruit. But other totes triched before the pins could grow to fruits, both with pasteurized and sterilized peat, I'm not using it anymore.

Bigger boxes and tubs gave me bigger fruits. The biggest fruits I had were in a 1L pp jar (spawned).

I reckon if I spawned it to straw+manure, they would thrive, because that's what CaptainFuture did to them for years and years. The nice horse manure is on the way, hopefully it will show good results.

TLDR - thin 100% peat casing works better than verm or coir for some genetics spawned to coir.



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Offlinechris77
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: Konbri] * 6
    #28323026 - 05/17/23 09:35 AM (8 months, 8 days ago)

second box of this culture is also slowly filling in.. i am very happy about the expΓ©rience. soaking them with a ultra fine mister feels great
:mushroom2::mushroom2::mushroom2:



--------------------
the observer is the observed
            j. krishnamurti


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InvisibleSoutherner
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: chris77] * 2
    #28323036 - 05/17/23 09:45 AM (8 months, 8 days ago)



Spawned 3/7 to coir/verm. These mofos will test your fuckin patience as can be seen on the struggling surface :lol:

Has anyone had any gnat problems with these? Since I’ve spawned them I’m removed at least two bisporus shoeboxes out due to gnats but these have not had a single problem. Sitting right next to a plant as well.


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Offlinepsilocybist
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: Southerner] * 1
    #28323046 - 05/17/23 09:50 AM (8 months, 8 days ago)

I have bought box of Β½kg diatomaceous earth, should a single gnat or any f***king insect dares to get close to my subs, I'll pour some like a casing on top. But this year so far haven't seen any.
Late summer they'll join the party.


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InvisibleKonbri
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: Southerner]
    #28323164 - 05/17/23 11:16 AM (8 months, 8 days ago)

Quote:

Southerner said:


Spawned 3/7 to coir/verm. These mofos will test your fuckin patience as can be seen on the struggling surface :lol:

Has anyone had any gnat problems with these? Since I’ve spawned them I’m removed at least two bisporus shoeboxes out due to gnats but these have not had a single problem. Sitting right next to a plant as well.




Given the bitternes of the fruits... Idk, that could be it.
My favourite edible Cantharellus cibarius is also very bitter when eaten untreated, and it's always untouched, at any age of the fruit, no larvae, no bugs, no holes. If I hunt in autumn, it's always them. I think they have much in common, really.


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Offline0t0lerance
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: Konbri]
    #28323207 - 05/17/23 11:56 AM (8 months, 8 days ago)

Quote:

Konbri said:
I reckon if I spawned it to straw+manure, they would thrive, because that's what CaptainFuture did to them for years and years. The nice horse manure is on the way, hopefully it will show good results.
[/url]




I actually pasteurized some bulk, straw/manure/coir a week ago.. but compared to 50:50 this stuff smells like shit after a week.. so tossed it outside with some bisporus spawn.


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InvisibleKonbri
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: 0t0lerance]
    #28323333 - 05/17/23 01:00 PM (8 months, 8 days ago)

Idk, when I prepped bulk for pans, I've put few liters of leftovers in a bag and tossed it on a cold balcony. I totally forgot about it, and just as I intended, I checked in a month or two, and there were some Inky Caps of different age, and it smelled kinda nice I'd say, damp, no shit. So next time when I was emptying lc jars, instead I took some myc mass with a fork and tossed it in the bag, the result was several decent flushes, the fruits looked as a cube in vacuum, though the lc was rw or z strain, can't remember.


Ah, sorry for OT. My batch of horse manure didn't smell bad, and after lime pasteurization together with straw and little coir it all smelled like hay to me. Did you use cow manure? I believe the key thing is that the manure should be composted.


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InvisibleBigwormS
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: Konbri] * 3
    #28323998 - 05/17/23 08:49 PM (8 months, 8 days ago)

After my second flush 3 weeks ago I'm going on my 3rd flush but they look different this time. Also I scraped the casing off since when I harvested I noticed the myc seemed to recognize better in the ripped off casing sections. I had hoped for a better pinset for this flush by doing so and it seemed to have worked. Not much but a slightly better pinset. These are starting to look like fat albino tits. Don't mind the off colors, they are stump remnants, not trich.


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InvisibleMojaveMyc
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: Bigworm] * 4
    #28324587 - 05/18/23 09:55 AM (8 months, 7 days ago)



Day 30 on this one, another MS from the fast-fruiting clone that went spawn to harvest in 30 days. I tested those fruits & agree with the sentiment that the speed comes at the expense of potency. I took 7g fresh & the experience was noticeably weaker than both of my trips (7-9g) on the slowpokes. They were still plenty strong, but the slowpokes introduced me to entities :lol:


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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: MojaveMyc] * 3
    #28324644 - 05/18/23 11:07 AM (8 months, 7 days ago)

Pins!


24 days from spawn. This is from a second flush clone from the fast line. Running slower than the first flush of the origin ms run.


--------------------
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How to succeed in mycology (and life) - know nothing, read everything, try something, and accept advice.
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InvisibleBigwormS
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: ReverendMyc] * 1
    #28324661 - 05/18/23 11:25 AM (8 months, 7 days ago)

So it occurred to me to look at my timeline with the these. I figured out that my variation of these from inoculation of grain to spawn to pin was 3 weeks for each step. Then from harvest till 2nd flush pins showing up was also 3 weeks.
Now same for the 3rd flush, 3 weeks for pins after harvest. Anyone else's timeline match the whole grow?


Edited by Bigworm (05/18/23 11:25 AM)


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Offlinepsilocybist
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: Bigworm]
    #28324722 - 05/18/23 12:16 PM (8 months, 7 days ago)

Then only God knows what linage mine are.
It's been around 3 months I'm working/waiting on this semperviva.
Hopefully the end result is worth this painful eternity.

Considering the room temp hasn't fallen below 23Β°C, the waiting is harder.


Edited by psilocybist (05/18/23 12:27 PM)


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