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OfflineJW123
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: chris77] * 2
    #28300868 - 04/30/23 08:58 AM (8 months, 25 days ago)

7 days. I have no idea though. I have a question for you fine people. I did some transfers last night, is the mycelium usually kind of rubbery? It reminded me of reishi mycelium.


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Offlinechris77
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: JW123] * 1
    #28300883 - 04/30/23 09:17 AM (8 months, 25 days ago)

Quote:

JW123 said:
7 days. I have no idea though. I have a question for you fine people. I did some transfers last night, is the mycelium usually kind of rubbery? It reminded me of reishi mycelium.



that would be quite the opposite of mine. mine was rather fine haired and  whispy like a mold
7 days. solid bet :wink:


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            j. krishnamurti


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Invisiblekirkeng
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: chris77] * 3
    #28300912 - 04/30/23 09:37 AM (8 months, 25 days ago)

Quote:

chris77 said:
Quote:

kirkeng said:
I’ll bite, I say 9 days!

Had some sugar cane left over from other projects started some new semp jars with the left overs, I don’t expect a difference but fuck it why not?




:incredible:
sugar cane? are you dabbling with distillation? craft rhum ?


ok, 9 days, got you.



No distillation, sober for two years, though my mind has crossed that idea. Zaps and caerus are the project.


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OfflinePsilosophy328
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: chris77]
    #28301007 - 04/30/23 11:09 AM (8 months, 25 days ago)

Hi Melgo,

I gave half of the print to a friend, who I've spent quite a bit of time growing with in the past. She started them on agar over the winter. Here's what she said

------------------------------------------------------------

The MS plate was very clean. I started a few jars of a RGS, RG, Oats mix after one transfer (T1). One jar looked very nice (no condensation, very white, fluffy clean growth to full colonization) and three of the jars had metabolites/condensation and some odd growth (more granular/powdery growth than standard mycelia in some areas). I checked for contamination by transferring grains from the fully colonized jars back to clean agar, right before I spawned two of the jars. One of the two plates with odd looking growth showed some oddness on agar (dense white "specs" sort of running along the path of the mycelia as it expands), but no obvious bacteria to speak of.

One odd-looking jar was spawned to bulk indoors, along with the "clean" jar, in separate trays at about 1:2.5 spawn to bulk ratio. The jars were spawned to a roughly 35/35/30 coir/straw/(rich topsoil/duff/oak leaf forest floor remnants from beneath an oak tree), which was pasteurized at 66-74C for 2 hours. That was back in March and they've been consolidating since just two days ago, when I cased them with a 50/50+ (screened peat/verm/lime flour - 45/45/10). To that mix, a small handful of garden soil was added and pasteurized. I was super tired when pasteurizing and had to go to bed, couldn't stay up to get to the planned 2-hr mark maintaining 60-70C. For certain I got to 1hr around 65 up to 72C for a short time, then put a towel around the pot and a ski jacket over that and went to bed. The mason jar was in the pot the full next day (pot lid never opened), and I opened it a day after that, and cased. After casing, I finally had the sense to smell the casing material in the jar, and I definitely wouldn't have used it if I had smelled it before using it. I think the garden soil might have had some compost, which maybe contained too much microbial life, or too much nitrogen. The casing in the jar smells like a musty basement (usually it ends up smelling like beautiful fresh garden/forest floor earth with this method). 2 days after casing, the trays smell fine. Hopefully it's not a mold issue and the life already climaxed in the consolidated bulk sub will influence and stabilize the casing.

The remaining two jars with odd growth were buried with some straw in separate locations in a shady area of my yard (equivalent USDA Zone 6a). Although the jars didn't smell odd (similar to mexicana but much milder) this was a "lazy" outdoor attempt since the jars seemed compromised.

For a slightly more "dedicated" attempt at outdoor cultivation, for a separate area outdoors, I pasteurized about a cubic foot of a nice mixture of dead grass roots/grass/rich soil clods, straw and a bit of aged compost/leaf litter, which was buried in a shady area of a lawn within a dry straw bed and covered with oak leaves and moss. The pasteurized "nucleus" sub was inoculated with three clean leftover "redundant/backup" agar plates, many of which were showing pins (4-8 weeks old). I just used a knife and cut up each plate into about 100 pieces like a grid, and spread the collective 300 pieces or so throughout the pasteurized sub. Using agar instead of grains should circumvent the spwan becoming a vector for animals/insects.  We are in the rainy season here, so conditions should be good for mycelial growth, but it's cooler than typical near Xalapa.


-------------------------------------

My friend and I share a similar philosophy in growing these truly "sacred" beings (compared to cubensis/other fungi subjected to capitalism,  black markets and recreational Western psychedelic use) - in that they should be treated with the utmost respect and every attempt should be made to give them a good, comfortable life when taking them out of nature. That is part of the use, the journey, the message, the relationship with sacred plants and fungi. Her grows reflect my attitudes in that regard.

As far as info regarding semperviva's natural habitat, I have a PDF but can't seem to upload it, even though it's below the size limit (used the "Upload Non-image" button)


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OfflineRoscoeReturnsS
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: 0t0lerance] * 3
    #28301058 - 04/30/23 12:02 PM (8 months, 25 days ago)

Quote:

0t0lerance said:

Hi Roscoe,

Sorry for the late reaction, had some other things going. But basically this is what I do too. In bags and pop them in a 100L EZ dial (I did add perlite to fill up the space). .

I wondered if you could says something about the following;
- what is your grain:coir ratio? I go for 1:2 but maybe I'm overdoing it spawn-wise (I add 2 pints of spawn to 2L of sterilized coir)
- How thick is your casing? I try as thin as possible but maybe a bit thicker could help.
- Do you slightly compress the bulk and/or casing? (I compress both a bit)
- Bulk slightly under or right on field capacity? (Here under)

Hope you can help out, I like to to reproduce these results, but I only had 2 bags like that (no clones, I have clones going right now.. still waiting for pins). Could have send a pm but maybe others would like to know too.

And.. do you use a bag-in-a-bag like freshprints? I had some massive side pinning with Nats lately, as I pulled the top of the bag along the side so I can reuse the bag.. but in doing so probably disrupted the mycelium and air got in. Sorry for all the questions, just love to know!




Hello! I am probably closer to 1:4 or 1:5 spawn to sub ratio. I knock up each bag with a pint of grain. My substrate is not straight coir either. I’ve been trying different substrate recipes for my Pan grows, and just use extra for these. All my subs contain some aged hpoo, some have straw, verm, or coir in different combinations.

My casing layer is usually 2-3mm thick regardless of what I’m using to case.

I do not compress substrate or casing, as I dump grain in the bag and squish it around to mix. The substrate is quite uncompressed, also the bags are not even very level at the surface. I sprinkle the casing on the surface and mist it in good, but do not compress it.

Substrate is just under field capacity. Casing is quite a bit under, and verm I sprinkle on dry, but immediately mist it quite heavily.

I am not using bag in bag. Just a single. I roll the tops down when I put them in the tub, and have not had major side pin issues. I got some, but most mushrooms were on the top. Recently I’ve been trying cutting the bag off just above substrate level when casing. Too early to tell if it’s better or worse. I’ll let you know. I did this because I noticed the stipes starting to get fuzzy with how humid I keep the tubs, how long they take to mature, and how restricted the airflow gets with the bag roll and the open caps in the way.


Edited by RoscoeReturns (04/30/23 03:58 PM)


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Offlinepsilocybist
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: chris77] * 1
    #28301290 - 04/30/23 03:54 PM (8 months, 25 days ago)

@chris77
11 days, if I win get me a print on paper, rather than alum foil. :bigblunt: :respect:


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OfflineRoscoeReturnsS
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: psilocybist] * 1
    #28301385 - 04/30/23 05:26 PM (8 months, 25 days ago)

Quote:

psilocybist said:
@chris77
11 days, if I win get me a print on paper, rather than alum foil. :bigblunt: :respect:




Curious why you prefer paper to foil.

Quote:

chris77 said:
Okay, lets have a contest :smile:
winner gets a print (if i can get one :wink:
worldwide

How many days till the first pin?







Ten days!


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OfflineMelgo
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: Psilosophy328] * 2
    #28301733 - 05/01/23 01:08 AM (8 months, 24 days ago)

Quote:

chris77 said:
26g fresh? do you have a tolerance built up? should be a massive hit otherwise..




He got his print from me, so I'm not surprised about the dosing. Since I need the exact same. They're super fast to pin, but not as potent as the other lineages. You can't have it all :lol:

Quote:

chris77 said:
Okay, lets have a contest :smile:
winner gets a print (if i can get one :wink:
worldwide

How many days till the first pin?




I say 30 days! :wink:

Quote:

JW123 said:
7 days. I have no idea though. I have a question for you fine people. I did some transfers last night, is the mycelium usually kind of rubbery? It reminded me of reishi mycelium.




Nope. The mycelium is super fine and whispy, for the fast lineage and the slow one too - I have both now.

Quote:

Psilosophy328 said:
Hi Melgo,

I gave half of the print to a friend, who I've spent quite a bit of time growing with in the past. She started them on agar over the winter. Here's what she said

[...]

My friend and I share a similar philosophy in growing these truly "sacred" beings (compared to cubensis/other fungi subjected to capitalism,  black markets and recreational Western psychedelic use) - in that they should be treated with the utmost respect and every attempt should be made to give them a good, comfortable life when taking them out of nature. That is part of the use, the journey, the message, the relationship with sacred plants and fungi. Her grows reflect my attitudes in that regard.

As far as info regarding semperviva's natural habitat, I have a PDF but can't seem to upload it, even though it's below the size limit (used the "Upload Non-image" button)




It's awesome to see these prints traveling around. Super interesting! Keep us updated with yours' and hers' progress :yesnod: I'll attempt outdoor grow this year too. I'm waiting for temperatures to be above 17°C tho'.

@Roscoe: thanks for detailing your setup, it's fascinating how these can grow in all kind of conditions :thumbup:


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Offline0t0lerance
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: Melgo] * 1
    #28301748 - 05/01/23 02:11 AM (8 months, 24 days ago)

It seems we have some more pins popping from the clone.. I've cloned the biggest subtropicalis cap, but I'm not seeing the same phenotype expression yet. I can already tell the stipes and pins are much thinner/less beefy than the original fruit (was grown on a straw/brf/verm substrate though). The bags are in a JCM FC, sorry for the poor quality:



So it does make me wonder if cvg is 'missing' something for the subtropicalis? I want the big fat fruits, not the tiny relative short spaghetti fruits.. also got slender stipes from Melgo's print, so it does make me wonder if it's a substrate thing. I'll repeat this with a different mix in the future as I saved the same genetics on cryo vials too.. looking at what roscoe has produced :rockon:.


Edited by 0t0lerance (05/01/23 02:13 AM)


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Offlinechris77
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: 0t0lerance]
    #28301763 - 05/01/23 02:47 AM (8 months, 24 days ago)

ok, so just the standings of the pin contest..

A.K.A 6
JW123 7
Kirkeng 9
RoscoeReturns 10
Psilocybist 11
Melgo 30 (!?!)


--------------------
the observer is the observed
            j. krishnamurti


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Offline0t0lerance
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: chris77] * 1
    #28301776 - 05/01/23 03:45 AM (8 months, 24 days ago)

Quote:

chris77 said:
ok, so just the standings of the pin contest..

A.K.A 6
JW123 7
Kirkeng 9
RoscoeReturns 10
Psilocybist 11
Melgo 30 (!?!)




I'll go for 14 :tongue:


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OfflineMelgo
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: chris77] * 1
    #28301814 - 05/01/23 05:12 AM (8 months, 24 days ago)

Quote:

chris77 said:
ok, so just the standings of the pin contest..

A.K.A 6
JW123 7
Kirkeng 9
RoscoeReturns 10
Psilocybist 11
Melgo 30 (!?!)




If you have the slow lineage, it could take a while :lol:


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Offlinechris77
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: Melgo]
    #28301818 - 05/01/23 05:16 AM (8 months, 24 days ago)

Quote:

Melgo said:
Quote:

chris77 said:
ok, so just the standings of the pin contest..

A.K.A 6
JW123 7
Kirkeng 9
RoscoeReturns 10
Psilocybist 11
Melgo 30 (!?!)




If you have the slow lineage, it could take a while :lol:



:headbang:well well :takingnotes:
in which way the two main strains are different? apart from one being faster..


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the observer is the observed
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OnlineA.k.aM
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: chris77] * 1
    #28301846 - 05/01/23 06:11 AM (8 months, 24 days ago)

I think the slower one tends to have a little bit larger caps but it could just be my mind playing tricks on me


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Offlinepsilocybist
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: A.k.a]
    #28301861 - 05/01/23 06:35 AM (8 months, 24 days ago)

We surely aren't talking about a single source for this specific sp. So who knows what genetic linage each of us has?
That aside, hasn't most of our grows started from spores?
Doesn't this mean we each have different and random unknown tickets in this MS lottery?


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Offlinechris77
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: psilocybist] * 1
    #28301876 - 05/01/23 07:06 AM (8 months, 24 days ago)

Sure thing, but if i understand it correctly, when a culture gets selsected time and time again, the lottery remains, but there a fewer "numbers" in the funnel, right?
hence natalensis, which is hardly domesticated at all atm throws out such a diversity of phenos, and others are rather uniform, or differ less.


--------------------
the observer is the observed
            j. krishnamurti


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Invisiblethe_chosen_one
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: chris77] * 3
    #28301937 - 05/01/23 08:17 AM (8 months, 24 days ago)

I take a few days off and come back to observationfest. :lol:
Love it!

Quote:

psilocybist said:
We surely aren't talking about a single source for this specific sp. So who knows what genetic linage each of us has?
That aside, hasn't most of our grows started from spores?
Doesn't this mean we each have different and random unknown tickets in this MS lottery?




We've been loading the balls in the machine with accelerated breeding practices. Cloning and isolation have made some traits recessive while making others dominant. That's my theory anyways.

Quote:

chris77 said:
Quote:

JW123 said:
7 days. I have no idea though. I have a question for you fine people. I did some transfers last night, is the mycelium usually kind of rubbery? It reminded me of reishi mycelium.



that would be quite the opposite of mine. mine was rather fine haired and  whispy like a mold
7 days. solid bet :wink:




I'm seeing both right now running ms from two sources. Sporeworks version is slow to colonize but extremely thick mycelium. Slower than Natalensis noc'd at the same time and almost as thick. All the jars look alike and are moving at the same pace. If my suspicion holds true I'm expecting lots of smaller fruits and faster pinning times. Probably a less long lived colony as well.
The spores Saralove provided are much more what I'm accustomed to seeing over the last decade. Thinner mycelium that colonizes reasonably fast then consolidates. I'd say about 40% faster than the Sporeworks in this case being one jar is already spawned to straw which is already half consumed. :lol: Even the comparative PF jar is easily 50% more colonized than the Sporeworks PF jars. From these I'm expecting a longer pinning time, larger but more sparse fruits and a really long colony life. Likely a year or more which is something I really miss.
:awesome2:


--------------------
"Luck favors the observant." - Workman



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Offlinepsilocybist
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: the_chosen_one] * 1
    #28302033 - 05/01/23 09:57 AM (8 months, 24 days ago)

I don't know what pheno mine is closer to.
It was whispy and fluffy on agar, perfect circle growth pattern, but on grians quite dense and thick, very much similar to cube mycelium. And it bruised dark cyan, almost black, after first shake. The grain bags are brick hard before shake, and now after recovery, again brick hard.
It's surprising how harder the myc network is compared to cubes.


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Offline0t0lerance
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: psilocybist] * 4
    #28302043 - 05/01/23 10:06 AM (8 months, 24 days ago)

Trying to make edibles.. subtrop.. first attempt.. tastes bad.. texture bad.. but.. works without a doubt.. aimed for 0,125gr per edible.. but for some reason feeling pretty high right now (maybe some calc mistake..)
:triplaunch:


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Offlinechris77
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: 0t0lerance]
    #28302073 - 05/01/23 10:32 AM (8 months, 24 days ago)

Quote:

0t0lerance said:
Trying to make edibles.. subtrop.. first attempt.. tastes bad.. texture bad.. but.. works without a doubt.. aimed for 0,125gr per edible.. but for some reason feeling pretty high right now (maybe some calc mistake..)
:triplaunch:




looks good though :smile:


--------------------
the observer is the observed
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