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OfflineRoscoeReturnsS
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Re: Official Hoogshagenii/Semperviva Thread [Re: Inkcap] * 1
    #28282327 - 04/17/23 04:17 PM (9 months, 7 days ago)

Quote:

Inkcap said:

Nice! Have you got a link to a post detailing your bottle and tub setup?




No link for what I’m doing. Muda bottles, I’ve used wide mouth pints and quart Ziplock containers with success. Substrate is 30% horse manure, 40% coir, 15% hydrated wheat, the rest is some straw if I have it lying around and some verm to get to field capacity. I use grain water to hydrate the coir and manure. Some bottles inoculated with LC, some with grain spawn. Some cased with verm, some with coir, some with 50:50 verm:peat. When they are colonized I take the lid off, case, and drop them in a Pasty EZ dial.

I think I’ll be harvesting those bottles tonight, so I’ll have more prints in a few days.


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Invisiblemyc_ousin_vinny
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Re: Official Hoogshagenii/Semperviva Thread [Re: RoscoeReturns] * 2
    #28282681 - 04/17/23 08:15 PM (9 months, 7 days ago)

Semp print is on the way. Pretty stoked about this after reading BigWorm’s trip report. Now I need to dive into this thread and learn the ways of Semperviva cult :rockon:


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Let's stay focused on what works consistently.

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OfflineMelgo
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Re: Official Hoogshagenii/Semperviva Thread [Re: Adas] * 5
    #28282862 - 04/17/23 10:15 PM (9 months, 7 days ago)

Quote:

chris77 said:
Quote:

Melgo said:
Quote:

Bigworm said:
I'll take potency over speed any day. I need to get my hands on Ambrose now. Lol




If the experience is the exact same, I'll take speed :lol:

I mean, I need 2.7 grams dried of Semp to have an amazing experience. A fat shoebox gives me ~40 grams dried, a bucket ~30. You get quite a few trips with such harvests.



40 g in how many flushes?




1 flush:



And TBH, I should've put "speed" in quotation marks. Because I grew one bucket this winter, and at 17°C it took 41 days from pinning for fruits to be fully matured and they didn't even drop spores :rofl:

Quote:

Adas said:
Well guys, I can send the Ambrose culture to some of you so no worries. I have recently sent out some dried agar pieces since the new trade rules allow it, I'll see if anything recovers for these folks. If it's a success I may as well share :shrug:




I didn't know that! It's a game changer!


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Offlinepesa
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Re: Official Hoogshagenii/Semperviva Thread [Re: Melgo]
    #28284125 - 04/18/23 05:42 PM (9 months, 6 days ago)

any idea, why Semperviva spores don't germinate after a year?
is shelf life of Semperviva way too small?


i got Semperviva but spores don't germinate on soft agar, neither in water.


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OfflineRoscoeReturnsS
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: pesa] * 14
    #28284137 - 04/18/23 05:49 PM (9 months, 6 days ago)



Making prints!



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OfflineSirPsycho
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: RoscoeReturns] * 1
    #28284140 - 04/18/23 05:55 PM (9 months, 6 days ago)

Quote:

RoscoeReturns said:


Making prints!





:cheer::bow2:


--------------------
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Balance in life is like running on ice.

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OfflineA.k.aM
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Re: Official Hoogshagenii/Semperviva Thread [Re: pesa] * 1
    #28284151 - 04/18/23 06:00 PM (9 months, 6 days ago)

Quote:

pesa said:
any idea, why Semperviva spores don't germinate after a year?
is shelf life of Semperviva way too small?


i got Semperviva but spores don't germinate on soft agar, neither in water.





I swabbed my first grow and nearly a year later it took me 6 swabs to get some germination. I think some lines just don’t last very long.


--------------------
LAGM2020


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Offlinepesa
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Re: Official Hoogshagenii/Semperviva Thread [Re: A.k.a]
    #28284352 - 04/18/23 07:26 PM (9 months, 6 days ago)

Quote:

A.k.a said:
Quote:

pesa said:
any idea, why Semperviva spores don't germinate after a year?
is shelf life of Semperviva way too small?


i got Semperviva but spores don't germinate on soft agar, neither in water.





I swabbed my first grow and nearly a year later it took me 6 swabs to get some germination. I think some lines just don’t last very long.




good to see you mate, thanx i will try again maybe use half of the print something will germinate from that


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InvisibleMojaveMyc
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Re: Official Hoogshagenii/Semperviva Thread [Re: A.k.a] * 3
    #28284357 - 04/18/23 07:30 PM (9 months, 6 days ago)



Finally got around to growing out the spores from that quick-fruiting clone. Primordia on day 11 :dancer: Also my first time growing these on millet. Keen to see if the speed comes at the expense of potency


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Offlinepsilocybist
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Re: Official Hoogshagenii/Semperviva Thread [Re: pesa]
    #28284657 - 04/18/23 10:49 PM (9 months, 5 days ago)

Quote:

pesa said:
any idea, why Semperviva spores don't germinate after a year?
is shelf life of Semperviva way too small?


i got Semperviva but spores don't germinate on soft agar, neither in water.




I just recently germinated 7~8 years old spores.
They germinated in 3 days, before other cubes of same age


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Offlinepesa
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Re: Official Hoogshagenii/Semperviva Thread [Re: psilocybist]
    #28284712 - 04/19/23 12:26 AM (9 months, 5 days ago)

Quote:

psilocybist said:
Quote:

pesa said:
any idea, why Semperviva spores don't germinate after a year?
is shelf life of Semperviva way too small?


i got Semperviva but spores don't germinate on soft agar, neither in water.




I just recently germinated 7~8 years old spores.
They germinated in 3 days, before other cubes of same age




thats great,
what you using?
agar or water?

i waited 30 days for something to germinate, now i just used half of the print made a spore syringe will see what comes out.


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Offlinepsilocybist
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Re: Official Hoogshagenii/Semperviva Thread [Re: pesa] * 1
    #28284722 - 04/19/23 12:52 AM (9 months, 5 days ago)

Spores were already hydrated in water, put on agar.
I don't know if it's pure luck or just how spores were, but cubes and pancyan, also 8 years old just germinated fine.
All within 3 to 5 days.
Just hydrated them in syringes (spore print to syringe) and waited for 3 days.
Then put on agar, injected to grains, and also just to increase my germination chance (which I assumed to be close to zero) I injected some spores into normal LC and also LC  with lowered pH.

Since all germinated, I discarded most, and just cleaned the mycelium on agar plates.


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OfflineMelgo
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: RoscoeReturns] * 2
    #28284726 - 04/19/23 01:04 AM (9 months, 5 days ago)

Quote:

RoscoeReturns said:


Making prints!






Gorgeous photos!
:fuckinawesome:


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Offlinepsilocybist
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: Melgo] * 2
    #28284754 - 04/19/23 02:36 AM (9 months, 5 days ago)

From the spores I mentioned above:


Haven't shaken them so far. Probably won't shake at all. The whole bags have become firm and nice.

These were Spore >>> (ab)Agar >>> two transfers >>> (ab)LC >>> grain bags.

EDIT:
Another bag of smaller grain bags :lol:


These smaller ones, each will be spawned into a bucket.


Edited by psilocybist (04/19/23 02:43 AM)


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Offlinechris77
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: psilocybist]
    #28285174 - 04/19/23 11:18 AM (9 months, 5 days ago)

my cased shoeboxes are moving forward and i can see mycelium pushing through the casing (patiently waiting for pins).
would you consider semperviva happier with more or less FAE? in other words, do i leave them latched till a pinset has arrived or do i unlatch now to trigger better pinning?
would be happy about some info.
substrate was rather below field capacity and is of course 100% colonized. casing ist verm/coir aroud field capacity ..


--------------------
the observer is the observed
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Invisiblekirkeng
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: chris77] * 1
    #28285460 - 04/19/23 01:55 PM (9 months, 5 days ago)

I lean towards more fae personally, I know some like low fae as well though. For me I like having good fae for consistent evaporation cycles to get my pin sets. But they don’t like being dry so finding the sweet spot of fae and moisture is the balance to look for. I’ll say given I like to run em them with higher fae, also bear in mind my higher fae is relative to an unmodified tub which very well could low fae by others standards, I do have to most heavily to maintain that moisture they like. But I do believe they benefit from good airflow. I run em with lower fae during colonization, once cased I bump it up. I used to not do that though and only dropped fae during colonization to minimize misting during that period.
Based on an unmodded tub my high fae correlates to about a .75-1in, rough estimate never actually measured, gap on the flipped lid. This is the same I do for pans.


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Offlinechris77
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: kirkeng]
    #28285487 - 04/19/23 02:12 PM (9 months, 5 days ago)

Quote:

kirkeng said:
I lean towards more fae personally, I know some like low fae as well though. For me I like having good fae for consistent evaporation cycles to get my pin sets. But they don’t like being dry so finding the sweet spot of fae and moisture is the balance to look for. I’ll say given I like to run em them with higher fae, also bear in mind my higher fae is relative to an unmodified tub which very well could low fae by others standards, I do have to most heavily to maintain that moisture they like. But I do believe they benefit from good airflow. I run em with lower fae during colonization, once cased I bump it up. I used to not do that though and only dropped fae during colonization to minimize misting during that period.
Based on an unmodded tub my high fae correlates to about a .75-1in, rough estimate never actually measured, gap on the flipped lid. This is the same I do for pans.



tx for your reply! wow, thats a huge gap. interesting. but then again i don't really know how to translate this to shoeboxes. but from what i read between the lines, keeping the boxes latched doesnt sound right in the context of your advice .


--------------------
the observer is the observed
            j. krishnamurti


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Invisiblekirkeng
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: chris77] * 2
    #28285498 - 04/19/23 02:18 PM (9 months, 5 days ago)

Yeah also bear in mind I run larger tubs which I find easier to maintain conditions with higher fae, I’d suspect dialing it back a bit to a shoebox would be advised, grain of salt on that though, that’s just what my instinct tells me I’ve never ran a shoebox so can’t comment from direct experience.


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Offlinechris77
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: kirkeng]
    #28285502 - 04/19/23 02:22 PM (9 months, 5 days ago)

cheers, i appreciate it.


--------------------
the observer is the observed
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Invisiblethe_chosen_one
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: chris77] * 5
    #28285549 - 04/19/23 02:47 PM (9 months, 5 days ago)

They'll grow under various conditions. Even invitro. Growing and sporulating are two different things though. You'll want good FAE to collect spores. My best spore collections were in big tubs and the GH. Not much in a cookie jar or similar dropped spores for me. At least worth collecting anyways. If they are chasing air they tend to drop less IME. 

The shelf life of spores for these should be around 5+ years average. They've lasted longer for me in an aqueous suspension. The oldest print I've worked with was about 7-8 years old.

I'm working on reviving Zapo Barrerae now and managed to shoot a few jars with some older Semperviva spores.. about 5 years old now. Germinated in 4 days and are growing well at the moment. Hopefully I can remember to update soon lol. Forgot my last run. :facepalm:


--------------------
"Luck favors the observant." - Workman



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