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Tweeq
Tweeq of Nature


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Re: Official Hoogshagenii/Semperviva Thread [Re: mycorry] 1
#27070202 - 12/03/20 03:00 PM (3 years, 1 month ago) |
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Waiting for my Semperviva spores to germinate rn so I'll tag along for this thread. Good call nosferatu
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Tweeq
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Re: Official P. Subtropicales/Semperviva Thread [Re: RiverBum] 1
#27078006 - 12/07/20 11:43 PM (3 years, 1 month ago) |
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Quote:
RiverBum said: Mini mono update. These fruits mature very slowly but this first flush is almost done! Harvested 65 grams fresh a few days before this pic was taken. Looking forward to seeing the total yield
 I should note that I have fruited these at temps as low as 68°F but this tub was fruited at 80° with similar results. Seems they are not too picky about temperature
Dude! Beautiful
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Tweeq
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Quote:
smalltalk_canceled said:

Ready to print?
Idk but ready to clone for sure. Looks like one huge cluster (and ready to eat too!)
We need that tripreport. Great job
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Re: Official P. Subtropicalis/Semperviva Thread [Re: coAsTal]
#27175426 - 01/29/21 12:15 AM (2 years, 11 months ago) |
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Mine looked exactly like that b4 grain. Colonizing rn
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Re: Official P. Subtropicalis/Semperviva Thread [Re: PitcherCrab]
#27183094 - 02/02/21 03:15 PM (2 years, 11 months ago) |
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Got three jars of ms mycelium of these just about ready to spawn to bulk. Eith cubes I like to do a top layer directly at the moment of making the tub. Any reason why that may or may not be a good idea with these?
I've read some stuff about people casing later and then scraping it off again. Sounded to me like a thin top layer should do it.
Not 100% sure about the sub yet. Probably coir supplemented with compost a little sand and verm and top layer coir verm sand.
Might try a mono and a dubtub if only 10% spawn is needed. Is it really? I may try different volumes with one jar each.
Any tips? I can still change plans now.. lol
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Tweeq
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Re: Official P. Subtropicalis/Semperviva Thread [Re: nosf3r4tu]
#27200752 - 02/12/21 01:49 AM (2 years, 11 months ago) |
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Do you guys wait with casing untill 100 pct colonized? I have three trays of these colonizing rn @ room temp. Peeked into ibe last night and it looks like it can go for a bit longer before case and fruit. They are now six days from spawn.
I'll update with pics
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Tweeq
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Re: Official P. Subtropicalis/Semperviva Thread [Re: nosf3r4tu]
#27200868 - 02/12/21 04:38 AM (2 years, 11 months ago) |
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Think I'll wait untill the surface looks all white and then case & fruit.
Sub is experimental (for me at least): For my three trays I used one 650 gram coco coir brick, about 1 liter of verm, a few handfuls of garden compost, a handful of straw, a handful of sand and some garden lime, pasteurized and divided over three separate 6 liter trays, not completely full. One myco liter of spawn per tray. Probably comes down to 20 pct spawn. Maybe a bit much but we'll see.

Will be cased with pasteurized coir verm sand
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Tweeq
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Re: Official P. Subtropicalis/Semperviva Thread [Re: mycorry]
#27201299 - 02/12/21 10:50 AM (2 years, 11 months ago) |
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Quote:
mycorry said:
Quote:
Tweeq said: Do you guys wait with casing untill 100 pct colonized? I have three trays of these colonizing rn @ room temp. Peeked into ibe last night and it looks like it can go for a bit longer before case and fruit. They are now six days from spawn.
I'll update with pics
With subtrops, my main failures have come from casing before colonization has finished. I don't know why but that's the pattern I have seen. However, this phenomena could just be caused by cultural practices so don't think that "Casing early=bad" as set in stone.
Still I will take this as 'why would I not wait a few days longer' You are growing these with success. I'm just trying. Be great if something cloneable comes of it.
I'll stick with my initial plan to wait untill I see nothing but colonized substrate, before I case. Thanks @mycorry for the response.
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Tweeq
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Re: Official P. Subtropicalis/Semperviva Thread [Re: mycorry]
#27207266 - 02/15/21 11:31 AM (2 years, 11 months ago) |
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Well I cased all three trays today (top layered is more accurate)

After casing, in fc:
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Re: Official P. Subtropicalis/Semperviva Thread [Re: mycorry]
#27207346 - 02/15/21 12:29 PM (2 years, 11 months ago) |
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I'm seeing a lot of different spawn ratios with this species. From 1:10 (on the other website) all the way up to 1:2. I went with 1:5.
Seems all over the place. Any thoughts on this, anyone?
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Re: Official P. Subtropicalis/Semperviva Thread [Re: coAsTal]
#27263980 - 03/22/21 01:25 AM (2 years, 10 months ago) |
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I have three trays. Cased February 15th. Haven't seen anything happen un any of them. Gave one of the trays an overnight soak last week. It put on a lot of weight but also no action to be seen.
Maybe I'll try and scrape the casing away from one too (and re-case). You think it's the the disturbance that makes them spring into action?
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Tweeq
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Re: Official P. Subtropicalis/Semperviva Thread [Re: coAsTal]
#27264153 - 03/22/21 07:37 AM (2 years, 10 months ago) |
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Quote:
coAsTal said: Could be-- i know it saved mine for sure
Well the reason I made three trays is to be able to mess with one or more of them so I might give this a shot. Idk how long is long with this species.. When you say scrape do you mean with something like a fork or how did you do it @Coastal? Your results with these are pretty epic.
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Re: Official P. Subtropicalis/Semperviva Thread [Re: coAsTal]
#27264253 - 03/22/21 09:11 AM (2 years, 10 months ago) |
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Thanks for such an elaborate answer. I will pasteurize some new casing for these. Last time I used coir/verm/sand.
The sub is a little bit of everything coir compost straw garden lime and also some sand, pasteurized. Rye grain spawn to sub approx 1:4.
This was when they were just cased after 10 days of consolidation. Tray in mono, on perlite at rt approx 21C

This is today:

Not much has happened. Those white dots have been there for quite a while but I can't make out if those were primordia or something. Casing is really thin
Quote:
coAsTal said:
Quote:
Tweeq said: Well the reason I made three trays is to be able to mess with one or more of them so I might give this a shot. Idk how long is long with this species.. When you say scrape do you mean with something like a fork or how did you do it @Coastal? Your results with these are pretty epic.
Semps can take forever to grow-- but I'm convinced that they are not always slow, just that they require certain conditions to kick out fruits. These flushes on this bag are averaging 2-3 weeks from harvest to what you see pictured. I started this bag last September with the original casing
Do you have any mycelium breaking up through the casing(s)? How thick/what is it made of? Temps? Conditions?
I found mycelium at the surface of the casing in less than 2 weeks from putting it in FC,

And primordia was starting about a week later in mid October:

But while it pinned heavy, it stalled completely. Here it was 5 weeks after the above pic on 11/20:

And 5 weeks later, just before new years:

That's when I took Mycorry's advice and scraped the casing then long-soaked the sub to try and solve the problem. I did this on 1/3 Here it was on 1/20:

12 days later 2/1:

I waited for spores, so here's the pre-harvest final pic on 2/7 of the first flush:

2nd flush on 2/22:

Pre-harvest 2nd flush on 3/4:

And that brings us back up to now. 
As you can see-- DRAMATIC difference after the scrape and soak.
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Re: Official P. Subtropicalis/Semperviva Thread [Re: seldom seen]
#27278006 - 04/22/21 10:38 AM (2 years, 9 months ago) |
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Still nothing much happening in my trays. One had some kind of contam on the surface so I chucked it. The two others look stalled and I'll have to try the scraping and recasing thing probably. Cased Feb 15th
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Re: Official P. Subtropicalis/Semperviva Thread [Re: mycorry]
#27296371 - 05/05/21 11:47 PM (2 years, 8 months ago) |
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Thanks for the insight @mycorry. I've spawned mine at about 1:5 (spawn:sub). Which is probably why they are taking so long.
Scraped the old casing off and added new 24th of April. No signs to see yet
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Tweeq
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Quote:
Ganodermalucidum said: Thank you Mycorry for alerting me to this thread, and killer contributions to the ever growing knowledge base on how to cultivate P. subtropicalis. Here are a couple trays of mine. I also have a clone pinning currently that did so at 18 days after fruiting.    
Wauw, 18 days..
My subtrop trays don't look like they are doing anything at all.
Spawned February 6th Cased February 15th (probably wat too early) Scraped dunked and recased April 24th
And looking exactly the same all this time. After how long do I give up? I've read about pinning starting anywhere from 14 days to over 100 days.
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Tweeq
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My two remaining trays have been given a burial in the garden. The casing layer that I re-cased with started to get some kind of mould. They had been in fruiting since mid February with 0 pins to show for anything.
Gonna have to re read the thread and see what I can improve. Any consensus on the best substrate for these? My mix of a little bit of everything looked nice but it didn't put out. At this point I have no clue what was the main problem. Could have been anything. PH? Substrate?, too wet, dry? first time casing was probably way too early in hindsight.
Soo starting over from spores again
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Tweeq
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Re: Official Hoogshagenii/Semperviva Thread [Re: mycorry]
#27326813 - 05/28/21 02:28 PM (2 years, 7 months ago) |
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Thanks for the feedback! A kind member supplied a fresh print so I can start over.
Quote:
mycorry said:
Quote:
Tweeq said: My two remaining trays have been given a burial in the garden. The casing layer that I re-cased with started to get some kind of mould. They had been in fruiting since mid February with 0 pins to show for anything.
Gonna have to re read the thread and see what I can improve. Any consensus on the best substrate for these? My mix of a little bit of everything looked nice but it didn't put out. At this point I have no clue what was the main problem. Could have been anything. PH? Substrate?, too wet, dry? first time casing was probably way too early in hindsight.
Soo starting over from spores again
I've had that happen before. It could be an unlucky non-fruiting culture.
I have tried all sorts of complicated and simple sub recipes with these and most seem to work out. However, whenever I have pinning problems I go back to simple BRF and straw/verm, or manure and straw until I see the results I want.
Subtrops have most of the same needs as cubes, with the exception of FAE(subtrops like less than cubes) and with subtrops there is no need for a "pinning strategy". So I'd say follow all the same PH and water content guidelines as cubes.
Starting over from spores sounds like a good idea. I usually run multiple separate germinations in parallel to ensure results.
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Re: Reply to Official Ps. Subtropicalis/Semperviva Thread [Re: Ambrose]
#27491090 - 10/03/21 01:41 PM (2 years, 3 months ago) |
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Quote:
Ambrose said: Here's that tray I posted previously. Guess maybe I should not have thrown out the culture it came from, oops . Pleasantly surprised to see what I think is some sporulation goin on, not sure when I should attempt to print, looks like a couple caps are maybe ready?
   
That's amazing! They just keep getting better
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Re: Official P. Subtropicales/Semperviva Thread [Re: Melliferous]
#27722076 - 04/05/22 12:49 PM (1 year, 9 months ago) |
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Quote:
Melliferous said:
 Couple second flushes, 15 qt shoebox
Amazing!
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