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Offlinebw86
Doesn't play well with others


Registered: 11/12/06
Posts: 5,937
Loc: 7b
Last seen: 23 hours, 6 minutes
Trusted Cultivator
Bacteria is a bitch
    #27069476 - 12/03/20 07:58 AM (3 years, 1 month ago)

This is my fourth transfer of aerial mycelium to try and shake this nasty bacteria. It all ways just seems to piggyback its way.
I have some gentamicin sulfate but online it says store at -20C and mine has been room temp for probably 5 years. Anyone think that worth it? Is it just garbage?
Im gonna go t5 and also go back to the original sample.


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OfflineAbabyphoenix
You
I'm a teapot User Gallery


Registered: 03/21/19
Posts: 1,015
Loc: Human Body
Last seen: 24 days, 20 hours
Re: Bacteria is a bitch [Re: bw86]
    #27069540 - 12/03/20 08:34 AM (3 years, 1 month ago)

What are you using to transfer?

Have you heard of needle transfers or anything Josex has written up about transferring away from contamination?
Heres his useful writeup on how to clean up that culture with a 21G needle, some BRF, and a sterile syringe.  I personally wouldn't waste my time with antibiotic things for agar as I've heard good sterile techniques are superior :thumbup:
Quote:

c10h12n2o said:
antibiotics are totally not necessary in this hobby, since you can clean up pretty much anything with well-timed transfers. i do keep gentamicin and chloramphenicol on hand, and occasionally use it, but it should absolutely not be a matter of course. the only thing i use it for is the final dish before making long-term master slants, to knock out any hidden bacteria before we put it into storage (just in case). i do not use antibiotics in the master slants, just the final plate before it, and usually dont use them even in these cases (they arent necessary). i have also used it before doing a2g (in the rare occasions when im trying to troubleshoot suspected hidden bacteria), or when a print is so incredibly dirty that the bacteria suppresses the myc from growing at all on standard agar (ive only seen this twice, and i think someone wiped their ass with the print).





Edit: Just realizing u have 7k posts. U probably know more than me lol


--------------------


:sporedrop::aum:Pho's MushiAventures::aum::sporedrop:
:heartpump:My '21 GLOG:heartpump:
:peace: LAGM 2022 :peace:



Edited by Ababyphoenix (12/03/20 08:38 AM)


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Offlinebw86
Doesn't play well with others


Registered: 11/12/06
Posts: 5,937
Loc: 7b
Last seen: 23 hours, 6 minutes
Trusted Cultivator
Re: Bacteria is a bitch [Re: Ababyphoenix]
    #27069677 - 12/03/20 09:52 AM (3 years, 1 month ago)

Only have 5k post, and 13-15 years of mycology under my belt :grin:
I appreciate you taking time to find a link.
Im gonna keep playing around until i secure a new melmec culture :thumbup:


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OfflineHaywire
Wetspot Wizard
Male


Registered: 12/29/13
Posts: 1,611
Last seen: 4 days, 18 hours
Re: Bacteria is a bitch [Re: bw86]
    #27069830 - 12/03/20 11:08 AM (3 years, 1 month ago)

have you tried the hot pour method?
that worked for me a couple of times.


--------------------
Ciao mamma, guarda come mi diverto


My grows :mushroom2: Outdoor patches


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Offlinebw86
Doesn't play well with others


Registered: 11/12/06
Posts: 5,937
Loc: 7b
Last seen: 23 hours, 6 minutes
Trusted Cultivator
Re: Bacteria is a bitch [Re: Haywire]
    #27069877 - 12/03/20 11:25 AM (3 years, 1 month ago)

I have not. Ill give that a try :thumbup:


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