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mushhead
Potato Devourer



Registered: 08/22/14
Posts: 2,218
Loc: Dimension H-231
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Re: Problem colonizing grated brf cakes to bulk [Re: ModularMind]
#27068627 - 12/02/20 04:48 PM (3 years, 2 months ago) |
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Are you forgetting that each and every spore is in of itself one piece of dna and when spores combine it becomes, oh shit, multi strained. Within the syringe is a solution which contains spores. It's a Spore Solution.
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ModularMind
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Registered: 02/09/10
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Re: Problem colonizing grated brf cakes to bulk [Re: mushhead]
#27068797 - 12/02/20 06:44 PM (3 years, 2 months ago) |
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One spore is not a strain. Germination isn’t guaranteed. It’s a spore syringe.
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mushhead
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Re: Problem colonizing grated brf cakes to bulk [Re: ModularMind]
#27068806 - 12/02/20 06:51 PM (3 years, 2 months ago) |
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monokaryotic spores become dikaryotic mycelium hyphae and at that point you have multiple strains of DNA fighting for space. Germination may not be guaranteed but then you only have inactive spore solution added to grain or brf. Upon germination and creation of dikaryotic mycelium there are multiple strains of dna. The spore syringe is literally an aqueous solution with suspended spores.
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ModularMind
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Registered: 02/09/10
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Re: Problem colonizing grated brf cakes to bulk [Re: mushhead]
#27068822 - 12/02/20 07:03 PM (3 years, 2 months ago) |
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Quote:
mushhead said: monokaryotic spores become dikaryotic.....
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mushhead
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Registered: 08/22/14
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Re: Problem colonizing grated brf cakes to bulk [Re: ModularMind]
#27068830 - 12/02/20 07:10 PM (3 years, 2 months ago) |
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I can see you're not willing to put forward the effort into understanding this.
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ModularMind
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Registered: 02/09/10
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Re: Problem colonizing grated brf cakes to bulk [Re: mushhead]
#27068848 - 12/02/20 07:23 PM (3 years, 2 months ago) |
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You keep giving yourself further allowance to prove your point, but at the same time contradict yourself.
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mushhead
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Re: Problem colonizing grated brf cakes to bulk [Re: ModularMind]
#27068871 - 12/02/20 07:37 PM (3 years, 2 months ago) |
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I never contradicted myself m8. I appreciate and love you anyways. Have a great night and do a bit of reading into mushroom genetics.
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ModularMind
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Registered: 02/09/10
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Re: Problem colonizing grated brf cakes to bulk [Re: mushhead]
#27068879 - 12/02/20 07:39 PM (3 years, 2 months ago) |
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Is a single spore a strain?
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mushhead
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Re: Problem colonizing grated brf cakes to bulk [Re: ModularMind]
#27068920 - 12/02/20 07:59 PM (3 years, 2 months ago) |
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A single spore is a monokaryote which contains one piece of DNA within it. The spore is nothing more than this. When germinated into Dikaryotic mycelium you have multiple dna strands. Dikaryotic mycelium is the "Strain" here, which we have multiple combinations of that strain with others. Spores within a liquid contained in a syringe is an aqueous solution of suspended spores. I'm referring to MS as the multiple strains of DNA that are produced by the combination of monokaryotes. These strands can be observed on any agar plate. MS being "Multispore" doesn't quite make sense to me because I think about multi-strain dna, defining it as a Spore Solution does. This is where we arrive at "Multi-Strain Cultures" as this referring to the multiple combinations of DNA within that culture.
Edited by mushhead (12/02/20 08:13 PM)
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ModularMind
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Re: Problem colonizing grated brf cakes to bulk [Re: mushhead]
#27068942 - 12/02/20 08:13 PM (3 years, 2 months ago) |
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Quote:
mushhead said: A single spore is a monokaryote which contains one piece of DNA within it. The spore is nothing more than this. When germinated into Dikaryotic mycelium you have multiple dna strands. Spores within a liquid contained in a syringe is an aqueous solution of suspended spores. I'm refering to MS as the multiple strains of DNA that are produced by the combination of monokaryotes. These strands can be observed on any agar plate. MS being "Multispore" doesn't quite make sense to me because I think about multi-strain dna, defining it as a Spore Solution does.
And yet a a multi-spore inoculation isn’t, at inoculation, multi-strain dna.
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mushhead
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Re: Problem colonizing grated brf cakes to bulk [Re: ModularMind]
#27068950 - 12/02/20 08:18 PM (3 years, 2 months ago) |
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Quote:
ModularMind said:
Quote:
mushhead said: A single spore is a monokaryote which contains one piece of DNA within it. The spore is nothing more than this. When germinated into Dikaryotic mycelium you have multiple dna strands. Spores within a liquid contained in a syringe is an aqueous solution of suspended spores. I'm refering to MS as the multiple strains of DNA that are produced by the combination of monokaryotes. These strands can be observed on any agar plate. MS being "Multispore" doesn't quite make sense to me because I think about multi-strain dna, defining it as a Spore Solution does.
And yet a a multi-spore inoculation isn’t, at inoculation, multi-strain dna.
You've just made my point, this is why its considered a Spore Solution because it hasn't yet met those requirements to be considered a multi-strain culture. At inoculation what we're injecting into the BRF or onto an agar dish is by definition spores in a solution of water, ergo a spore solution. After germination its a multi-strain culture of cubensis DNA
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ModularMind
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Re: Problem colonizing grated brf cakes to bulk [Re: mushhead]
#27069006 - 12/02/20 09:17 PM (3 years, 2 months ago) |
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Quote:
mushhead said:
Quote:
ModularMind said:
Quote:
mushhead said: A single spore is a monokaryote which contains one piece of DNA within it. The spore is nothing more than this. When germinated into Dikaryotic mycelium you have multiple dna strands. Spores within a liquid contained in a syringe is an aqueous solution of suspended spores. I'm refering to MS as the multiple strains of DNA that are produced by the combination of monokaryotes. These strands can be observed on any agar plate. MS being "Multispore" doesn't quite make sense to me because I think about multi-strain dna, defining it as a Spore Solution does.
And yet a a multi-spore inoculation isn’t, at inoculation, multi-strain dna.
After germination its a multi-strain culture of cubensis DNA
I’m glad you see where you were confused. There’s a lot to wrap your head around in mycology. You’re only 5 months post first cult. You’re doing fine.
P.S. Mycology isn’t limited to the cubensis species.
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mushhead
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Re: Problem colonizing grated brf cakes to bulk [Re: ModularMind]
#27069036 - 12/02/20 09:44 PM (3 years, 2 months ago) |
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Quote:
ModularMind said:
Quote:
mushhead said: A single spore is a monokaryote which contains one piece of DNA within it. The spore is nothing more than this. When germinated into Dikaryotic mycelium you have multiple dna strands. Spores within a liquid contained in a syringe is an aqueous solution of suspended spores. I'm refering to MS as the multiple strains of DNA that are produced by the combination of monokaryotes. These strands can be observed on any agar plate. MS being "Multispore" doesn't quite make sense to me because I think about multi-strain dna, defining it as a Spore Solution does.
And yet a a multi-spore inoculation isn’t, at inoculation, multi-strain dna.
After germination its a multi-strain culture of cubensis DNA
I’m glad you see where you were confused. There’s a lot to wrap your head around in mycology. You’re only 5 months post first cult. You’re doing fine.
I'm not confused. I appreciate the debate.
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bunsie
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Registered: 09/11/20
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Re: Problem colonizing grated brf cakes to bulk [Re: mushhead]
#27069279 - 12/03/20 04:27 AM (3 years, 2 months ago) |
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If there's a "multi-spore", why is there no term accepted term as "single spore"? Would get rid of the confusion in my opinion.
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jomanda1990
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Re: Problem colonizing grated brf cakes to bulk [Re: bunsie] 1
#27069377 - 12/03/20 06:53 AM (3 years, 2 months ago) |
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Can you stop derailing the thread about BRF TO BULK and make your own to discuss this awfully unrelated topic?
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