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Shop: North Spore Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies   Mushroom-Hut Liquid Cultures

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Invisiblebigfootscreepyuncl
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Registered: 11/15/20
Posts: 3,725
Loc: Gamehenge
Re: Advice needed - not getting anywhere [Re: mushhead]
    #27069136 - 12/02/20 11:30 PM (3 years, 2 months ago)

Quote:

mushhead said:
Ah so you're adding your micropore tape on and then PCing the jars.
There I believe is your issue. You need to PC without micropore and add it to the jars at the time of inoculation.




I'm not trying to hijack a thread, so I apologize in advance. I'll be doing my first BRF tek tomorrow and have been trying to find the answer to this and keep finding differing answers.

I'll be 'steaming' the cakes, not in a PC since Santa wont let me open my PC until Christmas morning...Anyway, what you're saying is that I should fill the jars, cover with foil, steam, move to SAB, inoculate THEN place the tape over the holes? Or tape the holes before I inoculate? This option seems like there's more risk of contamination than doing the tape after inoculation.

Sorry for asking such a dumb question but I've seen it every which way and I'd like this attempt to be successful. Cheers, mate!


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OfflineRoscoeReturnsS
Crotchety chode man
Male


Registered: 02/12/18
Posts: 1,756
Loc: State of Confusion
Last seen: 4 hours, 31 minutes
Re: Advice needed - not getting anywhere [Re: bigfootscreepyuncl]
    #27069245 - 12/03/20 02:46 AM (3 years, 2 months ago)

Quote:

bigfootscreepyuncl said:
Quote:

mushhead said:
Ah so you're adding your micropore tape on and then PCing the jars.
There I believe is your issue. You need to PC without micropore and add it to the jars at the time of inoculation.




I'm not trying to hijack a thread, so I apologize in advance. I'll be doing my first BRF tek tomorrow and have been trying to find the answer to this and keep finding differing answers.

I'll be 'steaming' the cakes, not in a PC since Santa wont let me open my PC until Christmas morning...Anyway, what you're saying is that I should fill the jars, cover with foil, steam, move to SAB, inoculate THEN place the tape over the holes? Or tape the holes before I inoculate? This option seems like there's more risk of contamination than doing the tape after inoculation.

Sorry for asking such a dumb question but I've seen it every which way and I'd like this attempt to be successful. Cheers, mate!




Are you doing the standard PF tek with a dry verm layer at the top of the jar? If so, don’t bother with the tape. It’s not necessary. Using MP tape as your only filter is not really the best way to go about this. It works well on tubs to regulate airflow, but a single layer on jars has been known to fail and allow contaminants in. Some use multiple layers, but that stuff gets kinda gross in the PC. If you use the dry verm layer, that is your filter and you don’t need anything else. If you don’t use the dry verm layer, or you’re doing grain jars, I would suggest SFDs or poly fill over MP tape.


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OfflineOvoidhunter
Buttery Crescent
I'm a teapot User Gallery


Registered: 09/17/16
Posts: 2,016
Last seen: 2 years, 9 months
Re: Advice needed - not getting anywhere [Re: RoscoeReturns]
    #27069260 - 12/03/20 03:27 AM (3 years, 2 months ago)

Your culture wasn't clean to begin with if you didn't do any transfers from the germination dish. Going straight from the germ dish is the same as not cleaning at all and injecting directly from the syringe.


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Offlinespandoos
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Registered: 05/26/20
Posts: 29
Last seen: 2 years, 9 months
Re: Advice needed - not getting anywhere [Re: Ovoidhunter]
    #27070213 - 12/03/20 03:12 PM (3 years, 2 months ago)

Quote:

Ovoidhunter said:
Your culture wasn't clean to begin with if you didn't do any transfers from the germination dish. Going straight from the germ dish is the same as not cleaning at all and injecting directly from the syringe.




I've used a bought syringe to BRF. Then syringe to agar to oats and used my last agar plate to create LC and used that on my last run. On the 40 plates I had 5 with contaminants but the rest all looked good.
The LC is clear with only the cloudy mys at the bottom.

Since my PC is very old...I'm wondering now if it is in fact reaching 15psi and if not is that's perhaps causing the extra water buildup.

Im ready for another run but I just can't see how low PSI could cause the extra wetness nor after 5 attempts that I've been unable to properly use foil :confused:


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OfflineFrugalFungi
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Registered: 10/12/19
Posts: 130
Last seen: 1 year, 10 months
Re: Advice needed - not getting anywhere [Re: spandoos]
    #27070610 - 12/03/20 07:20 PM (3 years, 2 months ago)

Those oats are way to wet my friend.


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Offlinespandoos
Stranger
Registered: 05/26/20
Posts: 29
Last seen: 2 years, 9 months
Re: Advice needed - not getting anywhere [Re: FrugalFungi]
    #27083261 - 12/10/20 11:58 PM (3 years, 2 months ago)

I've made up a new batch of oats. Boiled cooked them for 50min. Left to air dry for 24hours theb placed in the fridge as per BOBs oats prep tek for 12 hours then PC'ed for 90min.

I can see some condensation inside and some oats sticking to the sides but most move around freely as I turn the jar.

How does this look to the experienced eye since I can't see any difference to the previous attempts.

https://files.shroomery.org/files/20-50/766960317-20201211_084639.jpg
https://files.shroomery.org/files/20-50/766960450-20201211_084625.jpg
https://files.shroomery.org/files/20-50/766960592-20201211_084615.jpg
I want to inoculate with LC - thinking of 1CC per jar? These are 500ml jars.

Thanks.


Edited by spandoos (12/10/20 11:59 PM)


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