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waxbrain
That Fun Guy



Registered: 05/13/17
Posts: 678
Last seen: 2 years, 9 months
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questions about my old LC
#27068318 - 12/02/20 02:12 PM (3 years, 1 month ago) |
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back at it again, its been quite some time since my last grow, i assumed it was 2 years ago but its looking more like 3.
i have a couple old LCs from the last time i was growing, kinda forgot all about them in my fridge in my basement that we barely use.. first off - would it be unusual for them to still be viable? i have two that look like normal LCs with normal myc growth, is it possible that they're still good after this long if theyve been consistently kept cold in a fridge?
my third LC (which im providing a picture of) has a growth in it that i'm unsure of whether or not it is a contamination or if its a clump of pins growing inside.. could you take a look? im assuming contam but hey, doesnt hurt to ask. whether or not i should ditch my cultures, im giving cultivation another go, ive missed it very much. thanks for reading!
one last note: is there any new must see teks that have come about in the past couple years?
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mushhead
Potato Devourer



Registered: 08/22/14
Posts: 2,215
Loc: Dimension X-124
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Re: questions about my old LC [Re: waxbrain]
#27068392 - 12/02/20 02:42 PM (3 years, 1 month ago) |
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Your best bet would be to test all three with their own agar dishes. The ones that grow out clean you'll use the others you'll toss. I have had LC syringes still be viable after 2 years of refrigerated storage. I've only just barely gotten into making my own LC so the syringes I speak of were third party gourmet cultures.
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waxbrain
That Fun Guy


Registered: 05/13/17
Posts: 678
Last seen: 2 years, 9 months
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Re: questions about my old LC [Re: mushhead]
#27068409 - 12/02/20 02:53 PM (3 years, 1 month ago) |
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ill be honest i got lucky af with the LCs i have currently. they were MS to a sterilized LC jar and one of those jars ended up producing the best grow ive had to date. just hoping that one at least is still solid lol. all of my previous agar / isolation attempts have been failures, but now that im getting back into the hobby ill be giving agar another go for sure.
did you have any input on the pictured jar? that clump looks questionable to me so if its a guaranteed contaminated culture its not even worth trying.. but what's in there to the untrained eye could look like a pin cluster. im expecting a no but i figured id ask before i chuck the jar
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mushhead
Potato Devourer



Registered: 08/22/14
Posts: 2,215
Loc: Dimension X-124
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Re: questions about my old LC [Re: waxbrain]
#27068414 - 12/02/20 02:56 PM (3 years, 1 month ago) |
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OH ya no toss that broth somethings way off with it. We're really just testing the LC with agar, we're not really going to isolate anything or make transfers its just to see if the culture is clean.
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waxbrain
That Fun Guy


Registered: 05/13/17
Posts: 678
Last seen: 2 years, 9 months
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Re: questions about my old LC [Re: mushhead]
#27068421 - 12/02/20 02:58 PM (3 years, 1 month ago) |
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solid, thanks for the quick reply - not gonna lose sleep over it was just curious 
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waxbrain
That Fun Guy


Registered: 05/13/17
Posts: 678
Last seen: 2 years, 9 months
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Re: questions about my old LC [Re: waxbrain]
#27070241 - 12/03/20 03:37 PM (3 years, 1 month ago) |
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so heres my other alacabenzie LC.. you see those two little specks (around the middle and bottom right)? the myc looks beautiful but damn.. is there a possibility that isnt a contam or is this a 100% screwed LC? im gonna try it regardless but i wanted to post it anyway.. i do have 6ml of it in a syringe along with another with 2ml from the last time i used it that looks clean

this is the one that managed to do this, which was my best grow so far.

i also have another gt LC jar that looks great other than one speck that looks very similar to this jars questionable dot.
could these be clumps of spores that never ended up turning into myc or is that hopeful thinking - keeping in mind these are 3 year old refrigerated cultures. it looks like they might be slightly blue specks in both jars which i guess would counter my thoughts. 
thoughts?
oh, by the way, these havent been shaken/stirred for years.
Edited by waxbrain (12/03/20 03:38 PM)
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