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OfflineShnoobie
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Registered: 10/27/18
Posts: 32
Last seen: 2 years, 10 months
Safe to spawn?
    #27068123 - 12/02/20 12:31 PM (3 years, 1 month ago)

Hi guys, second attempt at grain using spore syringe. Looking around it seems like there might be bacterial contam (wetspot) in this. The jar on the left is a couple of days behind jar on right. It’s been about 3 weeks since spore inoculation and I shook jar right approx 5 days ago. A couple of questions:

1. Is it safe to spawn to bulk or should I chuck? I do rmr RR saying myc can outgrow a little bit of contam.

2. What are the risks (health etc) if I spawn and it is infected?



Thanks


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OfflineTheBoJim
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Registered: 07/23/20
Posts: 449
Last seen: 10 months, 8 days
Re: Safe to spawn? [Re: Shnoobie]
    #27068133 - 12/02/20 12:38 PM (3 years, 1 month ago)

What’s the worst that can happen? You waste some time and material. If you don’t mind that then go ahead and spawn.


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OfflineCapMeh
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Registered: 06/08/09
Posts: 924
Loc: The Holy Mountain
Last seen: 2 months, 28 days
Re: Safe to spawn? [Re: TheBoJim]
    #27068155 - 12/02/20 12:52 PM (3 years, 1 month ago)

The myc looks pretty good in the jars. As long as it doesn't have any milky liquid in it after full colonization, I'd spawn em.  :2cents:


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OfflineTheBoJim
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Re: Safe to spawn? [Re: CapMeh]
    #27068163 - 12/02/20 12:58 PM (3 years, 1 month ago)

I missed the part about you using spore syringe. That is a big no when it comes to grain. I would at least wait until the jars are 100% colonized if you want to increase your chances of getting anything out of them.


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OfflineShnoobie
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Registered: 10/27/18
Posts: 32
Last seen: 2 years, 10 months
Re: Safe to spawn? [Re: TheBoJim]
    #27068783 - 12/02/20 06:33 PM (3 years, 1 month ago)

Ah I didn’t realise that. Why is that? And what is safe to use to spawn to bulk from a syringe?


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OfflinePearl
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Registered: 05/28/20
Posts: 261
Last seen: 1 year, 3 months
Re: Safe to spawn? [Re: Shnoobie]
    #27069695 - 12/03/20 10:05 AM (3 years, 1 month ago)

Bacterial, but you will likely get fruits.

Spores to grain is a no no full stop as syringes are not clean.

Spores to agar, transfer until only myc remains, then transfer that to grain.


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