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EarlyMorningRain
Nocin'OnHeavensDoor


Registered: 05/18/11
Posts: 63
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Failed To Tamp Down (BRF Jars)
#27067719 - 12/02/20 08:19 AM (3 years, 1 month ago) |
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Seems some say to, others stress keeping substrate light an fluffy.
PCed with 2 layers of foil and micropore tape, all removed. Didn't realize the jars compacted and the dry verm layer shifted, so sub below is exposed. Already inoculated *with spore syringe.
Also have seen some people say to not be afraid to open jars. But according to everything else I've read, that would defeat the purpose.
Should I leave them(my leaning), or try to restore the dry verm layer from within the SAB, following sterile procedure with pasteurized verm?
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EarlyMorningRain
Nocin'OnHeavensDoor


Registered: 05/18/11
Posts: 63
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Failed, in general.

Didn't notice as they went from PC to SAB and foil was removed inside. Now that they are out it's apparent that water got in(sub more expanded at bottom, but no loose water).
Going to leave them, see what grows.
If I micropore taped the lids and had foil on(*for PCing*), can someone explain why this happened?
Again, I've seen people yell, 'TIGHT LIDS,' and have also heard lots to leave them slightly loose. I did the latter this time.
Edited by EarlyMorningRain (12/02/20 08:56 AM)
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Socrateshroom
сталкер


Registered: 09/05/18
Posts: 1,840
Loc: Westworld
Last seen: 17 days, 11 hours
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Do not open BRF jars after inoculation. You will expose too much surface area to possible contamination. Do not try restoring the dry verm layer.
Were your jars filled to the brim? I see a lot of space open under the lid.
Also, no need for micropore tape on brf jars. The inoculation holes give them the adequate gas exchange needed and the dry verm layer is a perfect filter.
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EarlyMorningRain
Nocin'OnHeavensDoor


Registered: 05/18/11
Posts: 63
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Okay, thanks. Thought that would be counterproductive.
Holes were taped for PCing. Actually had 2 layers of foil over lids, no condensation on foil or top of lid. Took tape off before inoculating. Leave off for GE.
Jar was topped with dry verm layer. Jar lids were slightly loosened before sterilizing. Thinking water must of gotten in, but need to figure out why.
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polaritymind
relaxed attention


Registered: 10/10/16
Posts: 994
Loc: Germany
Last seen: 3 months, 8 days
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Loose lids at most but dont open it. Maybe the water level in the pot was too high, I recommend at most 2/3 or 3/4 of jar height for the water level, with the jars in the pot.
If you dont run on the highest heat with the steam screaming out constantly that shouldnt let you run dry even in 90min.
-------------------- "to affirm life is to also affirm death" -Albert hofmann
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EarlyMorningRain
Nocin'OnHeavensDoor


Registered: 05/18/11
Posts: 63
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Re: Failed To Tamp Down (BRF Jars) [Re: polaritymind]
#27067820 - 12/02/20 09:19 AM (3 years, 1 month ago) |
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So if there's barely any verm layer, should I restore the micropore tape? Or let them colonize as is?
Quote:
Loose lids at most but dont open it.
Can you clarify what you mean?
I taped the holes, rubberside down, and screwed lids on-just not totally tight. Not even a 1/4 loose.
Then used two layers of foil over loaded jars, usually I use one.
I have an Instantpot Duo- the trivet comes up maybe .5".. so I added extra jar lids, I DID forget a layer of aluminum foil (but see multiple PF teks skip that).
Water came just to the top of empty jar lids, and was below the loaded jars. Ran for 100min (ten mins to get up to temp) at 15psi.
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