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Offline76Salvia
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Agar contam test question
    #27067549 - 12/02/20 04:38 AM (3 years, 1 month ago)

Hello~

I was doing DFA (dog food agar) for first time and due to a problem in the pressure cooker (not enought water), I had to stop it before 45min, it was about 30, apx.

as it was already done, and in order not to waste it, I did a test on different plates and containers.

How long should I wait to see if they are contaminated? (apx)
if a contamination appears, can I still use them for spore prints? to clean them on other dishes or smth.

It was a nice practice, at least hahaha

:crazy:

Thanks ~


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and I saw
the world that comes from us-



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Invisiblefilthyknees
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Re: Agar contam test question [Re: 76Salvia]
    #27067772 - 12/02/20 08:55 AM (3 years, 1 month ago)

30 mins of 15psi will be just fine for sterilizing agar.

You're fine to use the dishes right away. If contams do appear the dish cannot be used for anything.


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OfflineMySillyUmRuns
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Registered: 06/28/20
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Re: Agar contam test question [Re: 76Salvia]
    #27068083 - 12/02/20 12:06 PM (3 years, 1 month ago)

20-30 mins @15psi is plenty to sterilize your media. I usually let my plates sit for about 3 days to see if any contamination shows up. If not, you should be good to go. Also, I would not put spores on a plate that is showing contamination


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Offline76Salvia
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Registered: 02/10/20
Posts: 303
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Re: Agar contam test question [Re: MySillyUmRuns]
    #27068102 - 12/02/20 12:18 PM (3 years, 1 month ago)

Thank you very much, guys.
I really appreciate it

<3


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and I saw
the world that comes from us-



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