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OfflineInthepit
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Where are my edge contams coming from? - video
    #27065853 - 12/01/20 07:02 AM (3 years, 1 month ago)

I'm trying to reduce the number of edge contams from pouring.
I'm hoping someone will spot something wrong in pouring in this video.

Well a couple things went wrong, plates too hot, and not oriented beforehand.
There's a marker on the plates, I thought it might show something about the orientation.

But really where are the edge contams coming from...

                                   


So I've learned some basic stuff:
ISO/wipe gloves and forearms, mask, still air, slow movements, raised rack,
wiping the media bottle...

Some controversial:
Wipe the SAB with Clorox and let it sit awhile.
Damp Clorox towel under rack, wrap plates within the hour.
          :towelie:
Experimental:
Putting a Lysol wipe on the rack to work on.

Be the Latex     


December 2, 2020

Awesome responses so far:
Take foil off plates after wiping SAB down and let sit…
Line up markers.
Water bath for media bottle w/ thermometer
Leave foil on cap and make a little handle
Leave cap on, don’t wipe.
Don’t let fingers go past the edge of the top plate.
Pause after big movements.
No jostling!


Thank you Haywire, TheBoJim, and Meteah, AKA!!! 

" wouldn't wipe the lip of the bottle" - Haywire

"Try to lift the stacks just by pinching down on one of the lids
and even then try not to reach with the finger under the lids but
just on the outside." - Meteah

"1. let the air settle after big movement
2. reduce big movements" - TheBoJim

"but I’m really careful not to slide or jostle things around." - AKA

"the reason you're getting edge contam is because you're only exposing the edge of your plate while pouring,
and due to your poor SAB work the SAB is not really doing a good job at providing a STILL air environment. "



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:sporedrop: GLOSSARY  :sporedrop: ACROMYMS!   :sporedrop: GETTING STARTED :sporedrop:


Edited by Inthepit (12/02/20 07:28 AM)


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OfflineHaywire
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Re: Where are my edge contams coming from? - video [Re: Inthepit]
    #27065865 - 12/01/20 07:13 AM (3 years, 1 month ago)

I wouldn't wipe the lip of the bottle. If the bottle comes out of the PC, you screw the lid down tight and nothing should come into contact with the lip afer that.
I have no experience with SAB so someone correct me if I'm wrong.

Also, If they're only edge contams, then I guess it's not your pouring anyway. you can use parafilm or glad wrap to wrap the plates. usually edge contaminations come from condensation dripping down the sides of the plate giving spores/bacteria a ride.


--------------------
Ciao mamma, guarda come mi diverto


My grows :mushroom2: Outdoor patches


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OfflineInthepit
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Re: Where are my edge contams coming from? - video [Re: Haywire]
    #27065976 - 12/01/20 09:25 AM (3 years, 1 month ago)

Thanks Haywire! Thanks for checking out the video.

Ya condensation, I wonder if I stored the plates upside down.
I just saw a YouTube video about streaking and she said keep
them bottom up away from condensation.


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OfflineTheBoJim
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Re: Where are my edge contams coming from? - video [Re: Inthepit]
    #27067113 - 12/01/20 08:09 PM (3 years, 1 month ago)

few things i noticed that look like they can cause problems:

  • a lot of movement inside the sab(foil removal/plate and hand movement)
  • wiping the bottle
  • leaving the bottle open
  • brining that temp gun in and out


some tips:

  • let the air settle after big movement
  • reduce big movements
  • if you choose to work with hot plate/ bottle triple up on gloves
  • do bigger stacks to reduce movements


the reason you're getting edge contam is because youre only exposing the edge of your plate while pouring, and do to your poor SAB work the SAB is not really doing a good job at providing a STILL air environment.


--------------------


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InvisibleMateja
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Re: Where are my edge contams coming from? - video [Re: Inthepit]
    #27067354 - 12/01/20 10:35 PM (3 years, 1 month ago)

Quote:

Inthepit said:
I'm trying to reduce the number of edge contams from pouring.
I'm hoping someone will spot something wrong in pouring in this video.                                    [embed=350,250]https://youtu.be/KM2LnuN7JrE[/embed



I have to say seeing how much the plates were being handled and moved around I'm not surprised you have satellites. You are digging and wiping your fingers all over the underside of the lids. Your fingertips should really never reach under the rim of the lids because the underside of the lid Will go above the sterile media. Try to lift the stacks just by pinching down on one of the lids and even then try not to reach with the finger under the lids but just on the outside. (I'm not at home otherwise I could have takes a pic of me lifting  to show you what I mean about the fingers) here's a pouring video I made a while back maybe you can see in this foggy clip how I'm lifting the plates.



--------------------
Cakes inside Water Tub


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OfflineInthepit
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Pitty's poor SAB work! OMG! [Re: Mateja]
    #27067556 - 12/02/20 04:45 AM (3 years, 1 month ago)

Oh thanks to all y'all! This is great!
Thank you Haywire, TheBoJim, and Meteah!!!  :hatsoff:

" wouldn't wipe the lip of the bottle" - Haywire

"Try to lift the stacks just by pinching down on one of the lids and even then try not to reach with the finger under the lids but just on the outside." - Meteah
Yes, I get it! Awesome point!

"let the air settle after big movement
reduce big movements" - TheBoJim
Yes, this is part of the "how much" that's hard to describe,
I'll try reducing big movements further and pausing afterwards!

"the reason you're getting edge contam is because you're only exposing the edge of your plate while pouring,
and due to your poor SAB work the SAB is not really doing a good job at providing a STILL air environment. "

Oh! Mommy! LOL, ya baby bring it on, this is great!

And also condensation, I think just handling the plates more carefully, i.e. not sloshing condo around!








Edited by Inthepit (12/02/20 05:25 AM)


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OfflineHaywire
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Re: Pitty's poor SAB work! OMG! [Re: Inthepit]
    #27067568 - 12/02/20 05:11 AM (3 years, 1 month ago)

working in a SAB is a skill I don't possess.
the luxury of having a flow, I guess!


--------------------
Ciao mamma, guarda come mi diverto


My grows :mushroom2: Outdoor patches


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OfflineA.k.aM
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Re: Pitty's poor SAB work! OMG! [Re: Haywire]
    #27067627 - 12/02/20 06:40 AM (3 years, 1 month ago)

Idk how much difference it makes but I’m really careful not to slide or jostle things around. Like when you were moving the stacks of plates or spinning them to line them up, I would lift them enough to not be touching the rack during all that. It could just be paranoia but after realizing how many problems the vibrations from my phones speakers on top of the SAB caused I try to limit that stuff.


Also spraying the cleaner into the SAB to wipe the bottle will definitely stir some stuff up.



Lol I never thought about the weight of glass plates. Doing the first few in a stack sucks even with plastic ones I’m sure holding the stack of glass is a bitch.


--------------------
LAGM2020


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OfflineInthepit
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it's all about the jostle! [Re: A.k.a]
    #27067658 - 12/02/20 07:25 AM (3 years, 1 month ago)

Thanks AKA, :hatsoff: I can barely pick up 6 plates in the SAB safely!

Jostle, no kidding, I've added books on top of the SAB to stabilize it.
And that rack sucks, because the white stuff is sticky!


--------------------
:sporedrop: GLOSSARY  :sporedrop: ACROMYMS!   :sporedrop: GETTING STARTED :sporedrop:


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