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OfflineSteve9333
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Registered: 10/23/20
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Agar
    #27064568 - 11/30/20 12:50 PM (3 years, 1 month ago)

I’m a noob, I started everything from multispore syringes and have had some successes. 4 shoeboxes have worked out and 2 failed monotubs(lost to trich). I am now learning the importance of isolating on agar. Here I have 3 different agar trays. Should I be cutting out another sector or let these grow out?? And where would you sector?


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InvisibleLemgrub
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Registered: 04/02/18
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Re: Agar [Re: Steve9333]
    #27064592 - 11/30/20 12:59 PM (3 years, 1 month ago)

1st looks clean. 2nd and third look like there might be a spot of bacteria near the edge of myc. Third one also has a little denser morphology, may not mean anything, might mean a contam :shrug:

Either way, keep your cultures growing on new plates in the case of unforseen difficulties. I would probably take a sector from the first and then dice up the colonized agar and drop it in a jar.


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OfflineSteve9333
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Re: Agar [Re: Lemgrub]
    #27064684 - 11/30/20 01:40 PM (3 years, 1 month ago)

Thanks. The spot on the second one is actually on the counter. Why would a denser morphology maybe mean contamination? Should I transfer to peroxide agar if that’s the case???


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OfflineBig_Dub
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Re: Agar [Re: Steve9333]
    #27064704 - 11/30/20 01:51 PM (3 years, 1 month ago)

to me, it looks like a good time to make some transfers. try to make the transfers as tiny as possible


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InvisibleJosex
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Registered: 11/13/15
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Re: Agar [Re: Big_Dub]
    #27064737 - 11/30/20 02:10 PM (3 years, 1 month ago)

I like the look on those first 2 plates.


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