|
markkwrightt
Stranger
Registered: 11/30/20
Posts: 2
Last seen: 3 years, 1 month
|
Psylocibin Cubensus Monotub Tek Summary & Potency Query
#27064166 - 11/30/20 08:32 AM (3 years, 2 months ago) |
|
|
Hey All, I have Summarised the Method I have used to Produce some Albino A+ Mushrooms. I am finding Difficulty in understanding why I produced such large amount of big happy mushrooms with low potency.
I took a 2.5g dose as I am a first timer and I got a good mood boost a better perception but no visual and no where near a trip.
After Research I believe this could be due to Genetics and I was just Unlucky. however I believe accepting that as the problem is the easy solution and I would like to see if I have any areas where this problem could have occured.
Any assistance is appreciated. Thanks.
1.2kg sterilised Rye Grain bag 3ml of liquid culture 22L Bucket with Lid Dehydrator Micropore Tape Coco Coir Vermiculite Alcohol Spray/Wipes Gloves
1. inoculation
Inject Liquid Culture into bag through Unicorn Port 3ml Syringe will do 1.25 - 3kg Bags Cover hole with Micropore (Optional if Self Healing Port)
2. Colonisation & Consolodation
Temp - between 21 & 27 Degrees. Preferred 25 Degrees for faster Colonisation Light - No Effect Airflow - Not Required, Can be stored ina box to help regulate temperature
Colonisation - ~1-2 Weeks Consolodation - ~1-2 Weeks after Full Colonisation
Ensure Grain Bag is fully Colonised & Consolodated before Use. If Unsure Leave a Little Longer.
3. Substrate
Bucket Tek
~22L Bucket with Sealable Lid 1 Brick of Coco Coir 1.89L or 2 Quarts of Vermiculite 3.78L or 4 Quarts of Boiling Water
1. Add Brick & Vermiculite into Bucket 2. Add Boiling Water 3. Put on Lid & Leave for 2 Hours 4. Stir & Reseal 5. Leave to Cool Overnight or Less than 27 Degrees
4. Preparing Monotub
Line tub with Plastic Bag (Reduce Side Pinning and allow Easier additional Flushes) Tape up Holes with MicroPore tape to Allow Gas Exchange but not Fresh Air Exchange
1. Create a 1" Base of Substrate & Level 2. Break Up Fully Colonised Grain bag 3. Create Layers as Follow - 1/2 Grain Bag > Substrate Layer > Last of Grain Bag > Substrate Layer 4. Ensure that the layers are lightly mixed & Leveled 5. Finally Add a Casing Layer with Remaining of Substrate. (a layer of Undisturbed Substrate over the top of the Mixed substrate to Regulate Surface Moisture and Protect against Contamination) 6. Ensure Substrate is Level 7. Trim the Plastic Bag around the Perimeter of the Substrate 8. Substrate Should be between 3" - 4" in Depth
Improvements - Plastic Bag around Substrate Perimeter, Prevents Side Pinning & Allows Easier Cold Shock Flushes - Ensure Enough Substrate is left for the Casing Layer
5. Monotub Colonisation & Consolidation
Temp - between 21 & 27 Degrees. Preferred 25 Degrees for faster Colonisation Light - Little Effect, Can cover to reduce chance of early Pinning Airflow - Covered with Micropore to Ensure only Gas Exchange
Colonisation - ~2 Weeks Consolodation - ~1-2 Week After Full Colonisation
Ensure Full Consolodation is Achieved for Higher Yeilds & More Even Fruits. If Unsure Leave a Little Longer
Improvements - Heat Mat Below the Tub to Regulate Temparture. Radiatior Dried out the Sides too Much - Early Pinning Occured, Most Likely Due to too Much FAE. Cover Holes with Micropore to Stop FAE & Allow Gas Exchange only - Covering from Light may also Help Early Pinning However If no FAE then Pinning wont Occur.
6. Fruiting
Once Full Consolodation is Achieve then Fruiting can be started
Temp - between 21 & 27 Degrees. Preferred 22-23 Degrees for Bigger & Better Fruits Light - Light is a Secondary Pinning Trigger, Introduce Indirect Sunlight & Artificial Lighting Airflow - Holes Stuffed with Cotton Wool to Provide FAE Humidity - 95% ideally, However not a good indicator of Moisture Levels
Remove Micropore Tape from Holes & Plug with Cotton Wool. Ensure Holes at Bottom are Tightly Packed and Lightly Packed at Top
Misting 3-6 times a day - Small Droplets on the Surface (No Pooling) - Mist over the Box
FAE 3-6 Times a day - Fan Right After Misting for ~30 Secs - Crack Lid if Additional FAE is Required
The Evaporation of Surface Moisture is Primary Pinning Trigger. Allow to Evaporate and be Dry for short time between Misting
Improvements - Monotub Should Regulate itself Due to its design Thinking about doing a Smoke Test to Identify If there is Enough Natural FAE & Then Potenitally Test this is Practice. However I have to Ensure that I dont Provide too Much FAE through design & My own input - By Using Heat mat I am hoping this Provides better Surface temperatures for Pinning. (May also help with pooling at bottom of substrate)
7. Notes
- Yellow Metabolites are Anti-Biotic (Such as Penicillin) & can be Syringed up and Applied to a Contaminated part to Fight the Contamination - Mushroom Aborts can Still be harvested & Still contain the Active Component - Fluffy Base to Mushrooms & Wrinkled Caps = Too Much Moisture. a Little Fluff is acceptable - Thin Mushrooms Tend to lack FAE or Too High Temps - Harvest in One Pass if Possible. Harvesting Affects the Other Mushrroms & Slows growth even if they arent Directly connected. - If Substrate it too Dry, you can Bring back to Life by Pouring Water down the Gaps in the Perimeter
8. Next Steps
1. Multiple Flushes 2. Isolating Genetics For Liquid Culture & Aegar 3. Vertical Farming Techniques & Potential Implementation 4. Other Mushrooms, Shitake, Oyster, Lions Mane?
9. Previous Results
1. 1st Flush - 66g Dry
Edited by markkwrightt (11/30/20 11:34 AM)
|
Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
|
Re: Psylocibin Cubensus Monotub Tek Summary & Potency Query [Re: markkwrightt]
#27064244 - 11/30/20 09:39 AM (3 years, 2 months ago) |
|
|
A couple quick notes;
First your litres to quarts are reversed, a litre is larger than a quart.
Second shooting up premade grain bags is a bad idea. Luck was on your side there but don’t expect it always to be so.
Third, be careful with what you read. While it’s possible that metabolites can be aspirated and added back in some fashion, I wouldn’t expect that to hold much benefit. Most of the time if you can see the contamination it’s too late. Adding chemicals etc to the grow can have small benefits but are more likely to be worthless. It’s better to simply have an axenic grow from the beginning, rather than adding bandaids along the way.
|
markkwrightt
Stranger
Registered: 11/30/20
Posts: 2
Last seen: 3 years, 1 month
|
Re: Psylocibin Cubensus Monotub Tek Summary & Potency Query [Re: Pastywhyte]
#27064375 - 11/30/20 11:11 AM (3 years, 2 months ago) |
|
|
Thanks for the reply.
Litres to quarts is 1 Liters = 1.057 Quarts acorrding to google. I realise i typed this out wrong, thanks for the head up :-)
Surely a prehydrated and steriled bag of rye grain is one of the more reliable ways presumming you get a reliable supplier. why would this be a bad idea?
And I agree, These were just handy notes ive picked up that some people have had success with. most cases I would throw out and start again to be safe.
Edited by markkwrightt (11/30/20 11:12 AM)
|
Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
|
Re: Psylocibin Cubensus Monotub Tek Summary & Potency Query [Re: markkwrightt] 1
#27064459 - 11/30/20 11:48 AM (3 years, 2 months ago) |
|
|
Nothing is more reliable than doing something yourself so you can be sure it’s correct. Grain bags are notoriously easy to fuck up and you literally have no control over the grow at that point. If I was to buy a presterilized kit, it wouldn’t be a grain bag that’s for sure.
|
|
|
You cannot start new topics / You cannot reply to topics HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a 254 topic views. 19 members, 147 guests and 52 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ] |
|