Home | Community | Message Board

MRCA Tyroler Gluckspilze
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Left Coast Kratom Buy Kratom Extract, Kratom Powder For Sale   North Spore Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies   Mushroom-Hut Substrate Bags, Substrate Mix

Jump to first unread post Pages: 1
Invisibleredtailedhawk
Explorer of the Mystery
 User Gallery

Registered: 11/24/04
Posts: 559
Loc: The Old Continent
Using 12 months old mycelium stored in a petri dish at room temp?
    #27063884 - 11/30/20 01:33 AM (3 years, 1 month ago)

About 12 months ago, I've stored a few petri dishes of P. Mexicana mycelium at room temperatures. I wanted to see how long the mycelium will live. Surprisingly it's still alive and is sprouting tiny fruits within the petri dish itself.

I'm wondering if I can use this mycelium to inoculate new petri dishes, or would it be better to get a fresh start from spores, since the mycelium is quite old and was not refrigerated.

I'm assuming new run from spores might be more vigorous? Or is the fact that this being is still alive and fruiting in a petri dish, showing a trait worth preserving?

Thanks.


Extras: Filter Print Post Top
InvisibleJosex
#cheat_code
 User Gallery

Registered: 11/13/15
Posts: 8,995
Loc: Flag
Trusted Cultivator
Re: Using 12 months old mycelium stored in a petri dish at room temp? [Re: redtailedhawk]
    #27063893 - 11/30/20 02:23 AM (3 years, 1 month ago)

Pics help. But I think it's almost safe to assume that that culture must have lost a lot of vigor, I personally would start from spores, unless you want to do a kind of experiment or something.

Judging from what you said, the fact that that culture didn't produce stones in the plate nor grew mushrooms in all that time, makes me think it wasn't even an aggressive culture to begin with. If I didn't see pins or stones in a used plate within a few weeks I simply would not bother with that culture.


Extras: Filter Print Post Top
OfflineTattersail
Female User Gallery


Registered: 04/11/18
Posts: 285
Last seen: 1 year, 11 months
Re: Using 12 months old mycelium stored in a petri dish at room temp? [Re: Josex]
    #27063949 - 11/30/20 03:51 AM (3 years, 1 month ago)

I've recently taken transfers from unrefrigerated plates I abandoned back in March. They were decent cultures at the time, lots of pinning and nice mycelium growth, and were pretty dry by the time I revived them! But they seem to be doing fine so far on soft-ish agar, and I'll be putting some to grain in the next week or so. At the same time, I'm also making new cultures from spores just in case these don't work out well. It hadn't occurred to me that mycelium might have lost vigor while sitting around, I thought new agar would give it a new lease of life, but we'll see how it goes.


--------------------
LAGM2021
Trades

We may lose or we may win, but we'll never be here again


Extras: Filter Print Post Top
InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
 User Gallery
Folding@home Statistics
Registered: 04/30/13
Posts: 61,889
Loc: Milky way
Trusted Cultivator
Re: Using 12 months old mycelium stored in a petri dish at room temp? [Re: Tattersail]
    #27063965 - 11/30/20 04:30 AM (3 years, 1 month ago)

Should be fine


Extras: Filter Print Post Top
Invisibleredtailedhawk
Explorer of the Mystery
 User Gallery

Registered: 11/24/04
Posts: 559
Loc: The Old Continent
Re: Using 12 months old mycelium stored in a petri dish at room temp? [Re: Josex]
    #27064066 - 11/30/20 07:12 AM (3 years, 1 month ago)

Quote:

Josex said: the fact that that culture didn't produce stones in the plate nor grew mushrooms in all that time, makes me think it wasn't even an aggressive culture to begin with. If I didn't see pins or stones in a used plate within a few weeks I simply would not bother with that culture.




It did produce mushrooms (fruits). One about 6 months ago, and one just recently when the room temperature changed a bit.


Quote:

Tattersail said:I've recently taken transfers from unrefrigerated plates I abandoned back in March. They were decent cultures at the time, lots of pinning and nice mycelium growth, and were pretty dry by the time I revived them! But they seem to be doing fine so far on soft-ish agar, and I'll be putting some to grain in the next week or so. At the same time, I'm also making new cultures from spores just in case these don't work out well. It hadn't occurred to me that mycelium might have lost vigor while sitting around, I thought new agar would give it a new lease of life, but we'll see how it goes.




Thanks, it seems we'll be doing something similar. I'll transfer this old culture and start some new cultures from spores, just in case :smile:

Quote:

bodhisattaM said:Should be fine 




Thanks, I hope so :smile:


--------------------

"Who are you who live in all these many forms? You're death that captures all. You too are the source of all that's gonna be born. You're glory, mercy, peace, truth. You give calm a spirit, understanding, courage, the contented heart."


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Left Coast Kratom Buy Kratom Extract, Kratom Powder For Sale   North Spore Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies   Mushroom-Hut Substrate Bags, Substrate Mix


Similar ThreadsPosterViewsRepliesLast post
* Re: i this a picture of psilocybe mexicana fruiting in a petri dish? mycofile 4,739 10 02/05/00 11:45 AM
by Mycelium5150
* Whats with petri dishes??? Legoulash 1,647 11 01/29/03 04:19 AM
by debianlinux
* Some of my first petri dishes ever :) kykeon 3,122 7 10/26/02 12:24 AM
by kykeon
* Cheap Petri dishes on Ebay. RoadkillM 2,995 10 10/25/02 02:15 AM
by boxtop703
* how do you clone without a petri dish phishyfriend 2,120 5 06/15/02 11:39 AM
by Mushroom_Madness
* Re: Petri Dishes & Agar Q's Placebo 3,968 5 12/07/99 10:00 AM
by Anonymous
* agar and petri dishes Thworp 2,700 7 01/08/02 08:29 AM
by ar393
* Polystyrene Petri Dishes can you reuse? lostagain 3,554 5 10/10/02 06:59 AM
by happygrins

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
307 topic views. 41 members, 215 guests and 37 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.023 seconds spending 0.007 seconds on 12 queries.