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OfflineMcDominator
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Micropore tape is not a great filter
    #27061441 - 11/28/20 10:54 AM (3 years, 1 month ago)

Unpopular opinion. Micropore tape is not all it's cracked up to be. Every time I use micropore tape I get failures. Every time I don't use micropore tape (like jars with lids partially unscrewed) I get very few to no failures. Talking no pour agar.

I follow pastywhyte's tek to a T aside from using deli cups and putting the hole on the side. After running 20 plates in the PC for 45 mins @16 psi, letting it cool and depressurize on its own, and letting the plates sit for a week I typically get about 3 to 5 failed plates. Plates that I haven't even opened and have 3 layers of tape. The contam generally grows right near the hole. Some pics:







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I'm here to learn. I'm also willing to help. If I'm wrong on something, please call me out. I am not resistant to new information, but I always carry a healthy dose of skepticism.


“It would be possible to describe everything scientifically, but it would make no sense; it would be without meaning, as if you described a Beethoven symphony as a variation of wave pressure.”

-Albert Einstein


Edited by McDominator (11/28/20 10:56 AM)


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OfflineYeetusdeetus
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Re: Micropore tape is not a great filter [Re: McDominator]
    #27061468 - 11/28/20 11:12 AM (3 years, 1 month ago)

That’s a pretty popular opinion tbh lol. I think pastyplates do better with the hole on top so the foil and paper towel can act as an additional barrier to contaminants. That being said, micropore tape works for some people, maybe due to less airflow/sporeload, less handling or whatever.

I think if you really wanted to make mp work for you could probably hop through a couple extra hoops such as opening your pc in front of a flowhood, keeping your plates in a sanitized tote, etc.

But if you’re working with mini rounds chances are you don’t have a hood anyways

Pour agar ftw :micdrop:


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OfflineDuhrwood Stevens
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Re: Micropore tape is not a great filter [Re: McDominator] * 1
    #27061477 - 11/28/20 11:22 AM (3 years, 1 month ago)

Paper tape kinda sux. It leaves sticky residue and is delicate.

It's not waterproof and in the pc it's likely failing under the pressure equalizing between inside the plates and out.

You might try one layer while pc-ing and then add fresh dry tape as you take out of the pc to cover the blown out soggy tape?
:goodluck:


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OfflineZakkery
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Re: Micropore tape is not a great filter [Re: Duhrwood Stevens]
    #27061486 - 11/28/20 11:31 AM (3 years, 1 month ago)

Never found it to be a problem myself, even with only 1 layer my pasty plates don't contam. That's with the side hole and likely dropping them a few times.

Could it potentially be shitty tape? I assume there are different grades/quality of micropore tape.


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InvisiblebodhisattaMDiscordReddit
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Re: Micropore tape is not a great filter [Re: Zakkery]
    #27061498 - 11/28/20 11:39 AM (3 years, 1 month ago)

Micropore is a 3m brand so anything that isn't micropore isn't the right stuff anyway. All micropore is micropore


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Offlinetonz0Funguy
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Re: Micropore tape is not a great filter [Re: Zakkery]
    #27061507 - 11/28/20 11:43 AM (3 years, 1 month ago)

I use 1 layer of micropore and 1 layer durapore by 3m works like a charm. Yeah i can drop one and not worry with that combo lol scuff your plastic with a piece of sand paper it will adhere better. There is even micropore s which is surgical waterproof which works nicely also.


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Offlinestarbones
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Registered: 03/04/20
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Re: Micropore tape is not a great filter [Re: tonz0Funguy]
    #27061572 - 11/28/20 12:37 PM (3 years, 1 month ago)

Paper MP tape sucks my nuts as a jar filter. It might be fine for some but the residue and reaction it had with my lids and rings rusted them right the fuck out in a hurry. A royal cock to clean off to.


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OfflineMcDominator
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Re: Micropore tape is not a great filter [Re: starbones]
    #27061601 - 11/28/20 01:00 PM (3 years, 1 month ago)

Hmmm. I like the idea of trying durapore. And the idea of adding fresh tape after PC.

Glad, and not so glad, that I'm not the only one. Wish I could just get a damn flow hood! That would solve a lot of my problems right now.


--------------------
I'm here to learn. I'm also willing to help. If I'm wrong on something, please call me out. I am not resistant to new information, but I always carry a healthy dose of skepticism.


“It would be possible to describe everything scientifically, but it would make no sense; it would be without meaning, as if you described a Beethoven symphony as a variation of wave pressure.”

-Albert Einstein


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Invisiblealaskappalachian
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Re: Micropore tape is not a great filter [Re: McDominator]
    #27061682 - 11/28/20 02:04 PM (3 years, 1 month ago)

I use 3M cloth tape (2-3 layers) from the box stores all day and the only issues I've had were of no relation whatsoever.  :shrug:  Fuck paper tape though.  Always interesting seeing the wide array of results people get from different filter options. :bongload: I use mostly unmodified mainstays lids on 1/4 and 1/2 pint jars for all my prepours but have maybe 12 I still use with GE and for those it's MP always.  Different strokes, I suppose.


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"First we build the tools, then they build us."

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OfflinePrincessRosalina
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Re: Micropore tape is not a great filter [Re: alaskappalachian]
    #27061685 - 11/28/20 02:06 PM (3 years, 1 month ago)

I haven't had issues with 3m micropore yet. 1 layer on pastyplates and jars.


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OfflineKKVguitarist
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Re: Micropore tape is not a great filter [Re: PrincessRosalina]
    #27062261 - 11/28/20 11:04 PM (3 years, 1 month ago)

Yes the paper towel acts as azn additional barrier.

When I used to use these types of dishes for agar my holes would be on top and have ATHEIST 2 LAUER of micropore tape ,I think I did 4 tho to be honest.

Personally, buying prepoured pda petri is like $25 for 10 of em. For me it saves alot of headache and with 10 plates I can get 3 varieties from spore to liquid culture to 10 bags per dish so 30 growbags which produce 3 oz or skin the first few flushes for $25 in dishes.

But this is MY grow and my situation.  If you find this is too much of a pain maybe you should give it a try one time.

Just make sure to have parafilm


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OfflineGroo
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Re: Micropore tape is not a great filter [Re: KKVguitarist]
    #27062296 - 11/28/20 11:42 PM (3 years, 1 month ago)

guess what mofos parafilm is not 100% idiot proof either you can bump it even in a Petri dish cause micro-tears and get contemns through those micro tears


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OfflineSankhara
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Re: Micropore tape is not a great filter [Re: Groo]
    #27062411 - 11/29/20 03:59 AM (3 years, 1 month ago)

I always use 3 layers of micropore with out a single problem!


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OfflineMcDominator
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Re: Micropore tape is not a great filter [Re: Sankhara]
    #27062479 - 11/29/20 07:03 AM (3 years, 1 month ago)

I just had a thought guys. Finally some synapses fired :vaped:

The only reason there needs to be a hole in these is so they don't implode in the PC right? So once they come out of the PC, the hole is useless. I can just slap some duck tape over it and call it a day.

I'm not sure if agar needs FAE, I assume it needs a tiny bit. My containers probably have enough air in them that it won't be an issue I'm guessing.

If this doesn't work then I'm looking for a no mod no pour tek or inventing my own. I have an unopened container that I left out for about 6 weeks or so. It just started growing some lovely mold. So it's only a matter of time until these things contam.


--------------------
I'm here to learn. I'm also willing to help. If I'm wrong on something, please call me out. I am not resistant to new information, but I always carry a healthy dose of skepticism.


“It would be possible to describe everything scientifically, but it would make no sense; it would be without meaning, as if you described a Beethoven symphony as a variation of wave pressure.”

-Albert Einstein


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OfflineSankhara
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Re: Micropore tape is not a great filter [Re: McDominator]
    #27062537 - 11/29/20 07:50 AM (3 years, 1 month ago)

They need to have some sort of gas exchange (GE) for the mycelium to breathe. Petri dishes wrapped with saran wrap get their GE from the permeable (sort of) plastic.

You may get away without any but it is not recommended as far as i know, at least not for the long term.

Micropore is definetively not a great filter, but for many of us it does the job quite well. You could maybe check for storing them in a more sanitized place with less current? Or maybe try another brand? Do you touch the micropore when handling?

I always thought that deli cup caps leave room for sufficient GE though. Maybe give it a try with unmodded containers.

Hope it helps, have a nice day


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OfflineYeetusdeetus
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Re: Micropore tape is not a great filter [Re: Sankhara]
    #27062580 - 11/29/20 08:24 AM (3 years, 1 month ago)

There’s enough oxygen in the round without a filter, the hole is just so it doesn’t collapse.

I don’t think duct tape would work because it doesn’t get pc’d with the agar. It’ll be holding contaminants from your fingers from putting it on. Might have some success but probably not very consistent imo


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InvisiblebodhisattaMDiscordReddit
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Re: Micropore tape is not a great filter [Re: Yeetusdeetus]
    #27062586 - 11/29/20 08:26 AM (3 years, 1 month ago)

When it comes out of the cooker the mini round is full of water vapor not air. If you cover the hole it will have pretty little air content and mostly water vapor. If you wait to cover the hole then it will have unfiltered air.


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OfflineSankhara
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Re: Micropore tape is not a great filter [Re: bodhisatta]
    #27062615 - 11/29/20 08:42 AM (3 years, 1 month ago)

Another thing to consider is that humidity left in the filter could lead to contaminations


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Invisiblech0ppie
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Re: Micropore tape is not a great filter [Re: Sankhara]
    #27062619 - 11/29/20 08:44 AM (3 years, 1 month ago)

Make sure its '3M' micropore or GTFO :thumbup:


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OfflineMcDominator
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Re: Micropore tape is not a great filter [Re: bodhisatta]
    #27062624 - 11/29/20 08:47 AM (3 years, 1 month ago)

Yes it's 3m.


Quote:

bodhisatta said:
When it comes out of the cooker the mini round is full of water vapor not air. If you cover the hole it will have pretty little air content and mostly water vapor. If you wait to cover the hole then it will have unfiltered air.




Dang. That sounds right. I think I'm going to look for a screw top option and just unscrew a bit. Tired of messing with tape


--------------------
I'm here to learn. I'm also willing to help. If I'm wrong on something, please call me out. I am not resistant to new information, but I always carry a healthy dose of skepticism.


“It would be possible to describe everything scientifically, but it would make no sense; it would be without meaning, as if you described a Beethoven symphony as a variation of wave pressure.”

-Albert Einstein


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OfflineZakkery
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Re: Micropore tape is not a great filter [Re: McDominator]
    #27062767 - 11/29/20 10:08 AM (3 years, 1 month ago)

You may need the holy grail my friend.

I and many others can vouch for these containers. Tick many boxes.


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