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EarlyMorningRain
Nocin'OnHeavensDoor


Registered: 05/18/11
Posts: 63
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Cutting Out Healthy Myc/Contam Question
#27061292 - 11/28/20 09:00 AM (3 years, 1 month ago) |
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These are on the way to the compost. For future ref:
Can I cut out healthy myc from a stalled cake?

And in regards to the 2nd pic- Is the left side cake stalled, vs the right hand side contam?
Edited by EarlyMorningRain (11/28/20 10:22 AM)
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sonoramo
Contaminant



Registered: 02/27/19
Posts: 851
Loc: California, baby!
Last seen: 2 days, 8 hours
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Quote:
EarlyMorningRain said: These are on the way to the compost. For future ref:
Can I cut out healthy myc from a stalled cake?
Well obviously, you can. The real question is what you hope to achieve. I have been able to rescue a cake with a bad spot that occupied maybe 30 percent of it, and the remaining 70 percent still fruited. Much better success cutting a piece off with a sterile X-acto and dropping it on agar, then transferring clean growth to fresh cakes.
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EarlyMorningRain
Nocin'OnHeavensDoor


Registered: 05/18/11
Posts: 63
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Re: Cutting Out Healthy Myc and Contam? [Re: sonoramo]
#27061362 - 11/28/20 09:58 AM (3 years, 1 month ago) |
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My bad, *for transfer onto agar. Didn't consider chopping it up and setting in its own humidity chamber.
IIRC, established cakes are resilient to harmful contams? Trying to learn what to toss or not, and obv want to be safe.
Specifically the cake on the right of 2nd pic- smells normal but has wet spot and when dumped there was some gross liquid I've never seen. Bad bacteria?
Unfortunately I'm OCD about wasting not, and must utilize what I can.
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sonoramo
Contaminant



Registered: 02/27/19
Posts: 851
Loc: California, baby!
Last seen: 2 days, 8 hours
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Quote:
EarlyMorningRain said: My bad, *for transfer onto agar. Didn't consider chopping it up and setting in its own humidity chamber.
IIRC, established cakes are resilient to harmful contams? Trying to learn what to toss or not, and obv want to be safe.
Specifically the cake on the right of 2nd pic- smells normal but has wet spot and when dumped there was some gross liquid I've never seen. Bad bacteria?
Unfortunately I'm OCD about wasting not, and must utilize what I can. 
I didn't mean to suggest chopping the whole cake up. What I've done is just cut off the bad, soft, or squishy parts of the cake. "Cut" might even be an exaggeration because it just crumbles off with fingers. To clone from a cake, I used a sterile X-acto to expose a fresh surface from a still-viable section, then sliced a few mm-thick sections for the agar.
I know one guy who has great success inoculating BRF; he shreds the colonized cakes and mixes with coco coir. I've just never tried it myself. I moved on to using whole grains rather than BRF.
Mycelium can get weakened over time, especially as it runs out of fuel from the substrate. IME it's molds that move in when the mycelium gets weak, rather than bacteria. Often I'll cut off the "weak" parts to eek out a few more fruits. This works but typically doesn't get a lot more fruits, though.
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EarlyMorningRain
Nocin'OnHeavensDoor


Registered: 05/18/11
Posts: 63
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Re: Cutting Out Healthy Myc and Contam? [Re: sonoramo]
#27062702 - 11/29/20 09:34 AM (3 years, 1 month ago) |
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Would imagine cut applies if you're looking for clean margins?
There's solid growth on the three sides, think I inoculated a few too many drops?
Plan to slice some to experiment with agar, and crumble the rest into coir. Was going to do the former until reading your post.
Appreciate you taking the time and effort to explain- the true shroomy way.
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Pearl
Stranger


Registered: 05/28/20
Posts: 261
Last seen: 1 year, 3 months
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FYI - The not-colonized bits will just wash away under running water.
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