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OfflineSold Out Online
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Can I go to grain with these agar plates?
    #27061182 - 11/28/20 07:37 AM (3 years, 1 month ago)

Variety is Golden "Emperor" according to the vendor. They claim is a genetic isolation from Golden Teacher. I've grown them from spore print over the past few weeks.

Yellow plates are T3, blue is T2.

T2 is Nov. 17
T3 is Nov. 20



I feel like they're growing kinda slowly, but the temp might have been a bit low. Just moved them to a room with its own space-heater set to 70F.

Do they look alright to transfer to grain? Healthy? Should I wait until the plate is fully colonized?

Admittedly these aren't the best looking growth I've had. The sexiest and fastest-growing mycelium I've had is currently on T4, being transferred away from what I think was aspergillus. Sadly I forgot to take a pic before I hacked it up into 5 different plates.

I have enough coir, rye, and tubs for 3 grows, so I don't really need to worry about waiting for that batch if these look healthy enough to put to grain.

My first try was MS syringe to BRF jars to bulk and I messed that up and had to toss so some guidance here would be really appreciated.


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Offlinemad_dingo
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Re: Can I go to grain with these agar plates? [Re: Sold Out Online]
    #27061247 - 11/28/20 08:19 AM (3 years, 1 month ago)

Quote:

cdnpsychonaught said:
Variety is Golden "Emperor" according to the vendor. They claim is a genetic isolation from Golden Teacher. I've grown them from spore print over the past few weeks.

Yellow plates are T3, blue is T2.

T2 is Nov. 17
T3 is Nov. 20



I feel like they're growing kinda slowly, but the temp might have been a bit low. Just moved them to a room with its own space-heater set to 70F.

Do they look alright to transfer to grain? Healthy? Should I wait until the plate is fully colonized?





Looks alright from this side. Take a look from below: sometimes contams are hiding under the mycel, but are visible from the bottom.

If you decide they're ok, you can take a chunk of agar and turn it into liquid inoculant (LI) und use this to inoc your grain jars. Or use a chunk of agar directly. No need to wait for fully colonized plate, in fact thats a bad idea -- these kind of petri dishes often get contaminated through the gap over time, so the area close to the container wall is best considered dirty anyway.


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Re: Can I go to grain with these agar plates? [Re: mad_dingo]
    #27061267 - 11/28/20 08:35 AM (3 years, 1 month ago)

Quote:

mad_dingo said:
No need to wait for fully colonized plate, in fact thats a bad idea -- these kind of petri dishes often get contaminated through the gap over time, so the area close to the container wall is best considered dirty anyway.




OMG this is so helpful. I've been struggling with aspergillus around the edges of plates. Some of the spots not appearing until 1 week or more after a transfer. I was totally confused and really starting to doubt my procedures.


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InvisibleSir Pentinite
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Re: Can I go to grain with these agar plates? [Re: Sold Out Online]
    #27062113 - 11/28/20 07:54 PM (3 years, 1 month ago)

IMO none of the yellow plates look ready for grain and the blue one looks good, but another transfer would be better.


--------------------
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Re: Can I go to grain with these agar plates? [Re: Sir Pentinite]
    #27062159 - 11/28/20 08:47 PM (3 years, 1 month ago)

Can you help me out by expanding that a bit? Is it because the growth is uneven?


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InvisibleSir Pentinite
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Re: Can I go to grain with these agar plates? [Re: Sold Out Online]
    #27069914 - 12/03/20 11:46 AM (3 years, 1 month ago)

Yup, #1 & 3 look like they're growing away from something. Even if they're actually clean the slow side is obviously less vigorous.


--------------------
"I thought to myself 'Boy, I'm sure glad there's nobody here to see this because this is exactly the sort of thing that gets people riled-up and they assume you're dying and that something has to be done. Where if you're alone, you know, you either come through it or you die, but in any case you avoid the fuss.'"
- Terrence McKenna


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Re: Can I go to grain with these agar plates? [Re: Sir Pentinite]
    #27070393 - 12/03/20 05:13 PM (3 years, 1 month ago)

Thank you sir. That’s actually pretty helpful.

I ended up dropping each plate into its own rye quart. The blue one contaminated fast, with a day or two, got fuzzy all over and I could smell it through the poly filter. Lesson learned: transfer more. The other three are plugging along without any obvious issues yet. I’ll update with what comes of them for posterity.


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Invisiblemushhead
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Re: Can I go to grain with these agar plates? [Re: Sold Out Online]
    #27070415 - 12/03/20 05:34 PM (3 years, 1 month ago)

Hey OP.
If you pick up yourself some parafilm and use that to seal your plates you wont have to worry about contams or other impurities coming in through the cracks.
I actually find its really useful to allow a plate to come to full colonization before dropping it onto grain.
My reasoning behind this is it has a bigger surface area to colonize which allows you to shake your jars earlier.
You have to imagine that small wedge can only colonize so much surface area so fast, giving it a whole plates worth of surface area speeds up the process, as well as 90mm dishes will actually fit perfectly inside of a wide mouth quart jar.
I also have an unconfirmed hunch that it protects from contamination that could come from above during inoculation because the agar covers the top of your grains completely.
anyways you're doing great with the plates and you'll get the transfer process down. :thumbup:


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Re: Can I go to grain with these agar plates? [Re: mushhead]
    #27071238 - 12/04/20 05:48 AM (3 years, 1 month ago)

Is parafilm more reliable than saranwrap?

I think a second of these jars is starting to show signs of contamination.

Note to future noobs: if your plates look like that they probably aren’t ready for grain.



At the left of the jar there’s a bit of mycelium which is probably growing because of the vigorous shaking I gave the jars when I dropped in the agar. However, just below that there is a powdery texture on the grains. I’ll keep another day or so to be sure, but the last jar I saw some of that inside of became entirely overrun by said powdery substance pretty quickly, and is currently sitting on my deck in winter temperatures until I figure out how to dispose of its contents without contaminating myself or my house for future grows.


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Threads that have been useful to me:
Clean Spawn checklist for new growers


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OfflineRapjack
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Re: Can I go to grain with these agar plates? [Re: Sold Out Online]
    #27071246 - 12/04/20 05:58 AM (3 years, 1 month ago)

Quote:

cdnpsychonaught said:
Is parafilm more reliable than saranwrap?
.




No, both are considered equally reliable. I had issues with Parafilm forming cracks and causing contams (must've been an old roll) so I switched to Glad Wrap and never looked back. Love not having to cut strips before doing lab work.

A note: Make sure you are using Glad Wrap if you aren't already. Saran is a generic name for this kind of product but I don't know if the actual Saran brand wrap is the right kind of plastic.


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Invisiblemushhead
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Re: Can I go to grain with these agar plates? [Re: Rapjack]
    #27071482 - 12/04/20 09:37 AM (3 years, 1 month ago)

Quote:

Rapjack said:
Quote:

cdnpsychonaught said:
Is parafilm more reliable than saranwrap?
.




No, both are considered equally reliable. I had issues with Parafilm forming cracks and causing contams (must've been an old roll) so I switched to Glad Wrap and never looked back. Love not having to cut strips before doing lab work.

A note: Make sure you are using Glad Wrap if you aren't already. Saran is a generic name for this kind of product but I don't know if the actual Saran brand wrap is the right kind of plastic.



Parafilm has always served me proper and hasn't cracked on me yet in the years I've used it.
You can also use parafilm to graft cactus which is another reason I have it around.


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Re: Can I go to grain with these agar plates? [Re: Sold Out Online]
    #27072253 - 12/04/20 05:03 PM (3 years, 1 month ago)

3/4 contaminated.


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OfflineDr.Sparkle
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Re: Can I go to grain with these agar plates? [Re: Sold Out Online]
    #27072263 - 12/04/20 05:09 PM (3 years, 1 month ago)

Cling film/Saran is great, it’s cheap which makes it even better. Use a chop saw if you have one to hand to cut it to length, it melts the plastic where I cut it so you have to shave it off with a scalpel if you want it to come off the roll easy.

Parafilm is great also just more expensive, it lets the plates breathe apparently.


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