Home | Community | Message Board


Out-Grow.com - Mushroom Growing Kits & Supplies
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Getting Started

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: North Spore Bulk Substrate   Kraken Kratom Red Vein Kratom   Left Coast Kratom Buy Kratom Extract, Kratom Powder For Sale   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Original Sensible Seeds High THC Strains   Amazon Agar, Potato Dextrose

Jump to first unread post. Pages: 1
OfflineThreads2theSoul
Inquirer
 User Gallery

Registered: 09/10/20
Posts: 52
Last seen: 1 day, 14 hours
Why does my LME loose its color?
    #27060738 - 11/27/20 09:56 PM (1 month, 22 days ago)

Newbe here and curious why twice now I've followed Asura's agar recipe (LME, Agar (Telephone brand), water), poured them into and followed Alien's Holy Grail No Pour Agar unmodified containers. stacked the HGs in quart containers, vented 10 minutes, PCd for 1 hour at 15-17psi, etc.

Both times the agar goes in colored (blue or green) and comes out of the PC the color the agar was before I added coloring. I used Watkins brand, no artificial dyes, made with 100% natural vegetable juices and spices. Are there different kinds of food coloring?

Why does it loose it's color?


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
OfflineHaywire
Wetspot Wizard
Male


Registered: 12/29/13
Posts: 1,347
Last seen: 6 hours, 49 minutes
Re: Why does my LME loose its color? [Re: Threads2theSoul]
    #27060994 - 11/28/20 02:40 AM (1 month, 22 days ago)

the food colouring degenerates during the PC cycle.
use more or use different food colouring.


--------------------
Ciao mamma, guarda come mi diverto


My grows :mushroom2: Outdoor patches


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
Offlinemad_dingo
professional mango
 User Gallery

Registered: 08/19/20
Posts: 59
Last seen: 40 minutes, 7 seconds
Re: Why does my LME loose its color? [Re: Threads2theSoul]
    #27061025 - 11/28/20 03:39 AM (1 month, 22 days ago)

Quote:

Threads2theSoul said:
vented 10 minutes, PCd for 1 hour at 15-17psi, etc.
Why does it loose it's color?




LME Agar requires only 20-25minutes of Pressure Cooking, according to the lore on this site. Longer times might lead to caramelization.

If your food coloring doesn't survive even the shorter cycle, get different food coloring. E133, E131 (blue) and E104 (yellow) work for me


Edited by mad_dingo (11/28/20 03:44 AM)


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
OfflineMySillyUmRunsS
MycoNerd
I'm a teapot User Gallery

Registered: 06/28/20
Posts: 55
Last seen: 5 days, 19 hours
Re: Why does my LME loose its color? [Re: mad_dingo]
    #27061753 - 11/28/20 04:53 PM (1 month, 21 days ago)

all natural food dye like that looses it's color during the sterilization process. Also, 1 hour at 15psi is overkill


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
OfflineThreads2theSoul
Inquirer
 User Gallery

Registered: 09/10/20
Posts: 52
Last seen: 1 day, 14 hours
Re: Why does my LME loose its color? [Re: mad_dingo]
    #27061894 - 11/28/20 06:44 PM (1 month, 21 days ago)

I REALLY appreciate your time and quick response :regularshroom:
Quote:

mad_dingo said:
Quote:

Threads2theSoul said:
vented 10 minutes, PCd for 1 hour at 15-17psi, etc.
Why does it loose it's color?




LME Agar requires only 20-25minutes of Pressure Cooking, according to the lore on this site. Longer times might lead to caramelization.




~Could the 1 hr PC time be why no mycelium is growing? I applied an APE spore swab to my first batch of agar last Tuesday. It's now been four days and nothing. No mycelium, no contams, nothing. Shouldn't it be growing something by now?

~How can you tell if the agar has carmelized? I would assume you'd see some crystalization, maybe a look similar to honey that has started to turn to sugar? Except my HCs are smooth and clear but for a couple that look like there's a small amount of brownish sediment on the bottom like fine sand. Is that carmelization or is that unmixed agar or LME that was overcooked thus burned? When mixing it's hard to see through the dense liquid thus tell if EVERYTHING has been dissolved.


Edited by Threads2theSoul (11/28/20 07:37 PM)


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
OfflineCocaineBuffet
Stranger
 User Gallery


Registered: 08/30/19
Posts: 980
Last seen: 13 hours, 16 minutes
Re: Why does my LME loose its color? [Re: Threads2theSoul]
    #27062036 - 11/28/20 08:42 PM (1 month, 21 days ago)

Ape has been known to be very difficult to germinate but 4 days is a really short amount of time. I would look at it in like a couple weeks.

I have never overcooked my agar so I don't know what caramel-ization would look like or what impact it would have on my plates.

I have never had agar come out of the PC look like they went in. Everything just gets darker and uglier but that is just an aesthetic thing.


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
OfflineThreads2theSoul
Inquirer
 User Gallery

Registered: 09/10/20
Posts: 52
Last seen: 1 day, 14 hours
Re: Why does my LME loose its color? [Re: CocaineBuffet]
    #27062081 - 11/28/20 09:17 PM (1 month, 21 days ago)

Quote:

CocaineBuffet said:
Ape has been known to be very difficult to germinate but 4 days is a really short amount of time. I would look at it in like a couple weeks.




Thanks for the info. I'll keep my fingers crossed and be more patient.

Do you know if using a different type of agar (like PDA or something else) would have better results or should your room humidty or temp be within certain limits?


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
OfflineCocaineBuffet
Stranger
 User Gallery


Registered: 08/30/19
Posts: 980
Last seen: 13 hours, 16 minutes
Re: Why does my LME loose its color? [Re: Threads2theSoul]
    #27062084 - 11/28/20 09:18 PM (1 month, 21 days ago)

I don't have any information on agar types that work better with certain strains. I just use the same LME agar for everything.


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
OfflineHaywire
Wetspot Wizard
Male


Registered: 12/29/13
Posts: 1,347
Last seen: 6 hours, 49 minutes
Re: Why does my LME loose its color? [Re: CocaineBuffet]
    #27062344 - 11/29/20 03:12 AM (1 month, 21 days ago)

there is a thread by FrankHorrigan where he evaluates different types of agar.
I've been since mixing it up a bit but have not had any issues with using LME for years.

the general idea though is that you use a bit of the later substrate in the agar. then the mycelium can adapt it's enzymes for the future substrate. give it a head start so to speak. but I havent' tried this out and I can't vouch for the effectiveness.

your agar is caramelized when it turns a dark brown when using LME.


--------------------
Ciao mamma, guarda come mi diverto


My grows :mushroom2: Outdoor patches


Edited by Haywire (11/29/20 03:18 AM)


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Shop: North Spore Bulk Substrate   Kraken Kratom Red Vein Kratom   Left Coast Kratom Buy Kratom Extract, Kratom Powder For Sale   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Original Sensible Seeds High THC Strains   Amazon Agar, Potato Dextrose

Mushrooms, Mycology and Psychedelics >> Getting Started

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Ythan, geokills, Asante, Shroomism, RogerRabbit, mushboy, fahtster, wildernessjunkie, Ballerium, cronicr, bodhisatta
121 topic views. 1 members, 18 guests and 1 web crawlers are browsing this forum.
[ Print Topic ]
Search this thread:
MRCA Tyroler Gluckspilze
Please support our sponsors.

Copyright 1997-2021 Mind Media. Some rights reserved.

Generated in 0.035 seconds spending 0.004 seconds on 13 queries.