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OfflineNijamagi
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16 days no pins monotub - P. cubensis
    #27060643 - 11/27/20 06:14 PM (3 years, 2 months ago)

Why?







Edited by Nijamagi (11/27/20 06:17 PM)


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InvisibleYour Daddy
Registered: 11/13/20
Posts: 55
Re: 16 days no pins monotub - P. cubensis [Re: Nijamagi]
    #27060652 - 11/27/20 06:27 PM (3 years, 2 months ago)

Because they most likely aren't coming unfortunately. The super thick chunky myc is a sign of bacterial contamination and at that level/density it likely won't throw off any fruits. Sorry.


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OfflineNijamagi
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Re: 16 days no pins monotub - P. cubensis [Re: Your Daddy]
    #27060653 - 11/27/20 06:29 PM (3 years, 2 months ago)

Is it not just tomentose?


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OfflineNijamagi
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Re: 16 days no pins monotub - P. cubensis [Re: Your Daddy]
    #27060658 - 11/27/20 06:34 PM (3 years, 2 months ago)

Other have said this looks good...


Edited by Nijamagi (11/27/20 06:34 PM)


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InvisibleYour Daddy
Registered: 11/13/20
Posts: 55
Re: 16 days no pins monotub - P. cubensis [Re: Nijamagi]
    #27060669 - 11/27/20 06:42 PM (3 years, 2 months ago)

Posted it in multiple forums? Didn't like/trust the first answers?

:shrug:

That is still my answer / call though.

I'd ordinarily say give it a bit longer (especially if it's all you've got rn) because bacteria can be overcome if it's not severe and some cultures are slower than others, also that it's getting cooler now and that plays a big factor, and all kinds of other good jazz, but that myc doesn't look optimal to my eye and my eyes have seen plenty of my own failures to know when something is up.

But alas, I know like the others you won't take my word for it, so I guess you'll have to wait and see :cheers:


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OfflineShroomoisseur
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Registered: 11/16/20
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Re: 16 days no pins monotub - P. cubensis [Re: Your Daddy]
    #27060680 - 11/27/20 06:56 PM (3 years, 2 months ago)

Not experienced grower but the surface looks very moist, I think it needs to evaporate off and dry out a bit in the tub. Increase the gas exchange


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OfflineNijamagi
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Re: 16 days no pins monotub - P. cubensis [Re: Shroomoisseur]
    #27060687 - 11/27/20 07:02 PM (3 years, 2 months ago)

I sprayed it a few days ago because it started to look a little too dry


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OfflineYeetusdeetus
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Re: 16 days no pins monotub - P. cubensis [Re: Nijamagi]
    #27060691 - 11/27/20 07:06 PM (3 years, 2 months ago)

That tub looks no bueno to me friend


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OfflineNijamagi
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Re: 16 days no pins monotub - P. cubensis [Re: Yeetusdeetus]
    #27060702 - 11/27/20 07:25 PM (3 years, 2 months ago)

Could you elaborate?


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OfflineYeetusdeetus
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Re: 16 days no pins monotub - P. cubensis [Re: Nijamagi]
    #27060711 - 11/27/20 07:34 PM (3 years, 2 months ago)

It appears to be contaminated homeslice  :gangsta:

Spawn was probably dirty


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InvisibleYour Daddy
Registered: 11/13/20
Posts: 55
Re: 16 days no pins monotub - P. cubensis [Re: Yeetusdeetus]
    #27060739 - 11/27/20 07:59 PM (3 years, 2 months ago)

:ingodshands:


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Offlinehandy777
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Re: 16 days no pins monotub - P. cubensis [Re: Your Daddy]
    #27060757 - 11/27/20 08:14 PM (3 years, 2 months ago)

I have a similar looking tub and the exact same issue.

I just posted about it yesterday and received some responses today if you want to read them too.

I’m going to wait it out as I have no better options.

People have also told me it has bacterial contamination even though it looks bleached white and smells good. But they know more than I do so I’m trusting them.

The other thing is that I may have over-tended them and misted and fanned too much.

I’ll let you know if anything happens because I’m a few days ahead of you.

Yours aren’t A+ by any chance?


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OfflineYeetusdeetus
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Re: 16 days no pins monotub - P. cubensis [Re: handy777]
    #27060760 - 11/27/20 08:17 PM (3 years, 2 months ago)

Did your spawn look like a half eaten tub of cool whip? That’s a telltale sign of bacterial contamination. Some people describe it as an ice cream texture


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Offlinehandy777
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Re: 16 days no pins monotub - P. cubensis [Re: Yeetusdeetus]
    #27060781 - 11/27/20 08:35 PM (3 years, 2 months ago)

Quote:

Yeetusdeetus said:
Did your spawn look like a half eaten tub of cool whip? That’s a telltale sign of bacterial contamination. Some people describe it as an ice cream texture




It was really hard to break up. I remember this because I struggled to get it out of the jar, even with a spoon.

But I wouldn't say it had an ice cream texture.

This is it in the jar at the start and my similar looking tub.






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InvisibleYour Daddy
Registered: 11/13/20
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Re: 16 days no pins monotub - P. cubensis [Re: handy777]
    #27060785 - 11/27/20 08:40 PM (3 years, 2 months ago)

Yowza... That. Is. DENSE.

You've won the prize for the most "Ice Creamy" tub I've seen in a while. Congratulations!

R.I.P.

You'll get the next one!


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OfflineBLINKfan420
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Re: 16 days no pins monotub - P. cubensis [Re: handy777]
    #27060819 - 11/27/20 08:59 PM (3 years, 2 months ago)

Quote:

handy777 said:

It was really hard to break up. I remember this because I struggled to get it out of the jar, even with a spoon.




That is a SURE sign of bacterial contamination.  So the bacteria got into the spawn somehow. My guess it was too wet or what ever you inoculated with wasn't clean.

BLiNK


--------------------



All my pictures are drawn from imagination.  I don't even know what a mushroom is.


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InvisibleMateja
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Re: 16 days no pins monotub - P. cubensis [Re: Your Daddy]
    #27060844 - 11/27/20 09:13 PM (3 years, 2 months ago)

Quote:

Your Daddy said:
You've won the prize for the most "Ice Creamy" tub I've seen in a while. Congratulations!

R.I.P.

You'll get the next one!



And you win the prize of sounding like 'what you write in these posts is the most matter of fact thing out there but in fact you probably don't even have a clue about anything you've written' do you even have the most basic understanding of what a bacterial cell is, how it corresponds to water activity in its environment and water content within a growth medium and what kind of bacterial types there exists and to which extent they are able to affect the surface hyphae of a fungi far away from any kind of bacterial colony isolated away somewhere in some collection of water. This is btw not a critique towards you in particular, this can be said about 99.999% of posters in mush cult talking about bacterial colonies with such conviction and confidence. Literally sounds like the first pipedal mammal making a noise and pointing towards the night sky, as much as they understood about stars back then that's the current average understanding of microbes on this forum and I'm counted in that bunch as well, only difference is I took the time to learn the very very most basic things about the stuff I'm pretending to know about.


OP 2 weeks is still a bit early to definitely say 'R.I.P' to your substrate, I'd at this point add a very thin top layer of coir, close the lid shut for 30-40h on lay off the misting during that time. What this process will do is allow new myc to regenerate and 'grab' ahold of the newly added top layer of coir and depending on how this regeneration occurs therein will be some more definite answers about the health state of your substrate :thumbup: gl


--------------------
Cakes inside Water Tub


Edited by Mateja (11/27/20 09:15 PM)


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Offlinehandy777
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Re: 16 days no pins monotub - P. cubensis [Re: Mateja]
    #27060872 - 11/27/20 09:43 PM (3 years, 1 month ago)

Quote:

Mateah said:
Quote:

Your Daddy said:
You've won the prize for the most "Ice Creamy" tub I've seen in a while. Congratulations!

R.I.P.

You'll get the next one!



And you win the prize of sounding like 'what you write in these posts is the most matter of fact thing out there but in fact you probably don't even have a clue about anything you've written' do you even have the most basic understanding of what a bacterial cell is, how it corresponds to water activity in its environment and water content within a growth medium and what kind of bacterial types there exists and to which extent they are able to affect the surface hyphae of a fungi far away from any kind of bacterial colony isolated away somewhere in some collection of water. This is btw not a critique towards you in particular, this can be said about 99.999% of posters in mush cult talking about bacterial colonies with such conviction and confidence. Literally sounds like the first pipedal mammal making a noise and pointing towards the night sky, as much as they understood about stars back then that's the current average understanding of microbes on this forum and I'm counted in that bunch as well, only difference is I took the time to learn the very very most basic things about the stuff I'm pretending to know about.


OP 2 weeks is still a bit early to definitely say 'R.I.P' to your substrate, I'd at this point add a very thin top layer of coir, close the lid shut for 30-40h on lay off the misting during that time. What this process will do is allow new myc to regenerate and 'grab' ahold of the newly added top layer of coir and depending on how this regeneration occurs therein will be some more definite answers about the health state of your substrate :thumbup: gl




Not OP but I accidentally hijacked his/her thread because I’m in the exact same position and wanted to provide moral support.

I appreciate the knowledge and instructions. I will add a new layer of coir when I get the chance. Thank you!


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Offlinegabbk
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Re: 16 days no pins monotub - P. cubensis [Re: handy777]
    #27060884 - 11/27/20 10:02 PM (3 years, 1 month ago)

I'd keep waiting. When I began all my tubs were bacterial and took ±20 days from birth to pin. Really.


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InvisibleYour Daddy
Registered: 11/13/20
Posts: 55
Re: 16 days no pins monotub - P. cubensis [Re: Mateja]
    #27060900 - 11/27/20 10:18 PM (3 years, 1 month ago)

Down boy lol

I was being playful, and also encouraging that he would get the next one sincerely putting out good vibes about future tubs.

I might know a bit more than you think about various sciency tid-bits, but I am certainly very happy to let you enlighten us all. You clearly would be able to put it into words just fine. :wink:

I'm glad no critiques were leveled at me in particular lol

I am just offering my visual assessment as it was posted in a public forum. Yes, 2 weeks is early to for sure stick a fork in it, I did say that as well.

Quote:

Your Daddy said:
Posted it in multiple forums? Didn't like/trust the first answers?

:shrug:

That is still my answer / call though.

I'd ordinarily say give it a bit longer (especially if it's all you've got rn) because bacteria can be overcome if it's not severe and some cultures are slower than others, also that it's getting cooler now and that plays a big factor, and all kinds of other good jazz, but that myc doesn't look optimal to my eye and my eyes have seen plenty of my own failures to know when something is up.






I was calling it like I saw it, and I even thought to say I was sorry to have to say those too often over repeated words "it's bacterial", but I didn't actually say that part.

I know that stuff gets tossed around by people that haven't grown so much as a few cakes let alone stacks and stacks of tubs, but the many stacks and stacks I have done tell me that tub doesn't look destined to produce, but hey it does happen like I said before. Fingers crossed and good vibes to you OP. Both on this tub and the future ones. May the mush gods respect your sacrifice.

Sincerely excuse me if I was coming across as a know it all or mean for joking a tiny bit. I don't want any problems lol. I'm here to contribute to the community not kick up dust.

Mateah, is your assessment any different than mine has been throughout this thread? Inquiring minds want to know. Would you say that they aren't bacterial (whether it be OP or the next dude that posted asking), or that either of those tubs still look like winners??? Just curious if you actually thought different, or if it was just that you saw a member with a brand new account talking like they might know something and got triggered because so many new accounts do this and don't have any experience to back it up?

Maybe a little bit?

It's all love. :heart:


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InvisibleMateja
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Re: 16 days no pins monotub - P. cubensis [Re: gabbk]
    #27060903 - 11/27/20 10:21 PM (3 years, 1 month ago)

Bacterial or no, fruits will not form unless the environment allows them to.
My advice on the top layer of coir is to improve the immediate fruiting environment on the substrate surface, that's basically the only function of the top layer in this case.


--------------------
Cakes inside Water Tub


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InvisibleMateja
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Re: 16 days no pins monotub - P. cubensis [Re: Your Daddy]
    #27060931 - 11/27/20 10:44 PM (3 years, 1 month ago)

Quote:

Your Daddy said:
Mateah, is your assessment any different than mine has been throughout this thread? Inquiring minds want to know. Would you say that they aren't bacterial (whether it be OP or the next dude that posted asking), or that either of those tubs still look like winners???



This is the thing, in this post you're almost making me choose to either wrongfully lable that substrate surface as a "winner" or to utter the word "bacteria" pretending that I somehow can rationally or scientifically explain what kind of bacteria is causing that or why, or how it can be avoided and so on and so on. This is what I'd call pure speculative bullshit. Bacteria can not be seen unless you have a densely packed colony displayed in a medium making the colony extremely visible. For all I know the culprit that's causing the substrate surface to resemble ice cream could be yiest infection, could be mold, or could be some completely other kind of infection or even a disease making the cube colony sick. Hell, it could also very simply be environmental, the point is none of us knows anything for sure and that's why I personally avoid these widely used (useless) speculations that are grounded in absolutely nothing quantifiable cause all that does is confuse new growers further cause they learn basically nothing even after years of reading about other calling their substrates "bacterial" or "slightly" bacterial.



Ill give you another example: nothing makes me cringe more than seeing TC's not mention it but totally argue to point that their LC is "just a tad bacterial" or "slightly bacterial" completely unaware that there is no such thing as "just a tad bacterial" when live bacterial colonies are present in a liquid medium. The colony will firdt go through a short lag phase getting settled in the new environment and then in a matter of hours the log phase growth rate will have expanded even the most minute amounts of bacteria unto Narnia and back running circles around itself. In a matter of 24h bacteria will utterly and completely take over any kind of liquid growth medium, and that's counting the lag phase as well. Now imagine some growers are still arguing their point (speculating) about how their LC that's 14 days old is "slightly bacterial" :rofl: then when I go to explain to them scientifically exactly why this is impossible (as I've explained using the bacterial growth phase terms) some posters will at times try to counter with "yeah but what if bacteria was introduced only at the latter stage of the LC colonization"? Like when? Lol. During the last 2.5h of your 14 day LC colonization? Hahahaha cell division occurs every 15-20min for most bacteria lol yeah so good luck explaining the theory behind the scenario that your weeks old LC is "slightly bacterial". But posters will often still kick and scream and try to fabricate even more elaborate speculations and why? Because speculations are seemingly what the vast majority of the basic understanding here is about stuff. :bongload:


And I'm glad you didn't take offense at my rants, this is just me frustrated and feeling entitled and special cause I've been lurking microbiology forums since this summer so I'm basically just a prick :shrug: (but nevertheless with a slightly higher knowledge about bacteria than most here) *entitlement intensifies*


--------------------
Cakes inside Water Tub


Edited by Mateja (11/27/20 10:51 PM)


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InvisibleYour Daddy
Registered: 11/13/20
Posts: 55
Re: 16 days no pins monotub - P. cubensis [Re: Mateja]
    #27060987 - 11/28/20 12:33 AM (3 years, 1 month ago)

:havesomescience:

I will be happy to see you around the boards sir :smile:

You are very right of course.

I, for one, will try to be more careful throwing out blanket terms when doing visual assessments. Rather than throwing out a term I can't actually verify from eyeballing something I will instead just focus on what the visual cues tell me from my own personal experience as far as what tub will likely produce or have a better shot at it versus others etc.

You are right it does muddy the waters both for people just starting, as well as those advancing. I'll do my very bestest to not contribute to the problem! :heart:

Although from what I know of the shroomery you must spend a good amount of your time cringing and being peeved lol :lol: Seems like a ship that you'd have a hard time turning :rofl:


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OfflineNijamagi
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Re: 16 days no pins monotub - P. cubensis [Re: Mateja]
    #27062665 - 11/29/20 09:12 AM (3 years, 1 month ago)

Appreciate the advice man. At this point I have accepted the tub is probably lost - a day after posting 2 small areas of yellow bacterial growth appeared and it's starting to smell a little musky. I'll try a very thin coir casing anyway but it looks like I'm back to square 1. At least I've learnt a lot from this experience.

I was being extremely sterile in my whole procedure and I'm and not convinced it has this texture because of bacterial contamination, to me it just looks very very tomentose and so did in the jars before I threw them in the monotub, and I was very picky with which jars I chose to use - they all smelt like mushrooms and looked healthy as far I can tell.

I have discovered something rather interesting and pretty intuitive though that I think I'm going to utilise in my next run. This is that I did not throw away a few jars and lo and behold the remaining colonised grains started sending off rhizomorphs to search for food. I had a hard time getting rhizomorphic mycelium and perhaps this is a true way to induce it: putting a grain or two of rye inside a petri dish and inoculating it, then transferring the resulting rhizomorphs to agar! I'm just not certain that it wouldn't revert back to the tomentose type in the agar, will have to just try it and see.


Edited by Nijamagi (11/29/20 09:15 AM)


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OfflineNijamagi
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Re: 16 days no pins monotub - P. cubensis [Re: gabbk]
    #27062683 - 11/29/20 09:22 AM (3 years, 1 month ago)

My tub has gone bacterial too, although I'm not sure this is the reason for the texture.

Gives me a glimmer of hope though, cheers for the concise insightful input!


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