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InvisibleWall.E
Bacteria's Bitch
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Registered: 06/05/20
Posts: 2,860
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Re: Post Your Cultivation Picture of The Day [Re: fahtster]
    #27059616 - 11/27/20 04:20 AM (3 years, 5 months ago)

I've seen way more impressive natalensis flushes lol. At least I got something worth cloning? :shrug:



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Life’s shit, but I’m loving it


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InvisibleMunchauzen
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Registered: 06/22/11
Posts: 14,346
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Re: Post Your Cultivation Picture of The Day [Re: Munchauzen]
    #27059618 - 11/27/20 04:33 AM (3 years, 5 months ago)

Quote:

Munchauzen said:
so heres a thing I've been doing. got tired of loading dehydrator treys. poked some holes in the bottom of my jar box that I use for harvesting. put a kitchen cooling rack over top. sat on top of my presto. has worked great with 24 hour runs!



I just loaded that batch, I'll reply to this post when they are dried. they come out like a solid block of shrooms.





they look nice 😋

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Invisiblefunky123
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Registered: 08/27/17
Posts: 257
Loc: Austria
Re: Post Your Cultivation Picture of The Day [Re: Munchauzen]
    #27059619 - 11/27/20 04:35 AM (3 years, 5 months ago)

Quote:

Munchauzen said:
Quote:

Munchauzen said:
so heres a thing I've been doing. got tired of loading dehydrator treys. poked some holes in the bottom of my jar box that I use for harvesting. put a kitchen cooling rack over top. sat on top of my presto. has worked great with 24 hour runs!



I just loaded that batch, I'll reply to this post when they are dried. they come out like a solid block of shrooms.





they look nice 😋




I assume the drying temp was around 100°C - right?


--------------------
:nyan: Log for my 2nd Grow (with pix and chronology)

To shut up GIFs in Firefox: Go to about:config and set 'image.animation_mode' to 'none'. Then restart Firefox.
For any other browser (especially Chrome): Uninstall browser. Switch to Firefox. See above.

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InvisibleMunchauzen
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Re: Post Your Cultivation Picture of The Day [Re: funky123]
    #27059620 - 11/27/20 04:37 AM (3 years, 5 months ago)

I doubt it. I have no idea what the actual temp is but thats very hot. the presto dehydrator has no settings, its either on or off.

Edited by Munchauzen (11/27/20 04:37 AM)

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Invisiblefunky123
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Posts: 257
Loc: Austria
Re: Post Your Cultivation Picture of The Day [Re: Munchauzen]
    #27059625 - 11/27/20 04:42 AM (3 years, 5 months ago)

Quote:

Munchauzen said:
I doubt it. I have no idea what the actual temp is but thats very hot. the presto dehydrator has no settings, its either on or off.




I googled presto and just found devices for cooking and assumed this is some crazy hack. But searching for "presto dehydrator" led me to the specs. According to those it's set to 74°C.


--------------------
:nyan: Log for my 2nd Grow (with pix and chronology)

To shut up GIFs in Firefox: Go to about:config and set 'image.animation_mode' to 'none'. Then restart Firefox.
For any other browser (especially Chrome): Uninstall browser. Switch to Firefox. See above.

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OfflineeLeSDenes
Mycelium Expander
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Re: Post Your Cultivation Picture of The Day [Re: funky123] * 1
    #27059708 - 11/27/20 07:10 AM (3 years, 5 months ago)

This clone of RW grows many small shrooms :laugh:

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Offlinejomanda1990
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Re: Post Your Cultivation Picture of The Day [Re: Munchauzen]
    #27059714 - 11/27/20 07:16 AM (3 years, 5 months ago)

Quote:

Munchauzen said:






Holy shit haha
Seems you found a new drying tek


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OfflineAdas
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Re: Post Your Cultivation Picture of The Day [Re: jomanda1990] * 2
    #27059716 - 11/27/20 07:17 AM (3 years, 5 months ago)

Cyan clone 3rd flush.


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OfflineFailboat
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Re: Post Your Cultivation Picture of The Day [Re: Munchauzen]
    #27059766 - 11/27/20 08:14 AM (3 years, 5 months ago)

Quote:

Munchauzen said:


they look nice 😋



:notsureif:
I think I've seen that implemented before. Saw a few bags my homie had recieved that looked unnaturally tidy like that lol.
:super:

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InvisibleJosex
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Re: Post Your Cultivation Picture of The Day [Re: Josex]
    #27059924 - 11/27/20 10:14 AM (3 years, 5 months ago)

Quote:

Josex said:


PE pinset. :popcorn:






Getting there.

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OfflineBrownBear
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Re: Post Your Cultivation Picture of The Day [Re: Josex]
    #27059967 - 11/27/20 10:41 AM (3 years, 5 months ago)

Nice tub, josex.

I had some really nice, rhizo growth on a few TYC plates. I was able to isolate some thick ropey strands from them. This is the result so far from one of those transfers.
The rest of them look very similar.


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Invisiblefunky123
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Registered: 08/27/17
Posts: 257
Loc: Austria
Re: Post Your Cultivation Picture of The Day [Re: BrownBear]
    #27059977 - 11/27/20 10:51 AM (3 years, 5 months ago)

Quote:

Iambrownbear said:
Nice tub, josex.

I had some really nice, rhizo growth on a few TYC plates. I was able to isolate some thick ropey strands from them. This is the result so far from one of those transfers.
The rest of them look very similar.






The conventional wisdom here is that rhizomorphic growth is not a genetic trait but a response to environmental conditions and hence cannot be isolated.


--------------------
:nyan: Log for my 2nd Grow (with pix and chronology)

To shut up GIFs in Firefox: Go to about:config and set 'image.animation_mode' to 'none'. Then restart Firefox.
For any other browser (especially Chrome): Uninstall browser. Switch to Firefox. See above.

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OfflineBrownBear
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Re: Post Your Cultivation Picture of The Day [Re: funky123]
    #27060030 - 11/27/20 11:21 AM (3 years, 5 months ago)

Quote:

funky123 said:
Quote:

Iambrownbear said:
Nice tub, josex.

I had some really nice, rhizo growth on a few TYC plates. I was able to isolate some thick ropey strands from them. This is the result so far from one of those transfers.
The rest of them look very similar.






The conventional wisdom here is that rhizomorphic growth is not a genetic trait but a response to environmental conditions and hence cannot be isolated.




I'm just doing this for fun to keep agar work from becoming a boring routine. I've read that rhizo growth doesn't perform any better than tomentose growth. But that is about as much as I know. Thanks for the response. How would I go about isolating a single strain? What would be the easier type of growth to isolate? Or does it not matter?

Edited by BrownBear (11/27/20 11:22 AM)

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Invisiblefunky123
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Posts: 257
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Re: Post Your Cultivation Picture of The Day [Re: BrownBear]
    #27060070 - 11/27/20 11:53 AM (3 years, 5 months ago)

Quote:

Iambrownbear said:
Quote:

funky123 said:
The conventional wisdom here is that rhizomorphic growth is not a genetic trait but a response to environmental conditions and hence cannot be isolated.




I'm just doing this for fun to keep agar work from becoming a boring routine. I've read that rhizo growth doesn't perform any better than tomentose growth. But that is about as much as I know. Thanks for the response. How would I go about isolating a single strain? What would be the easier type of growth to isolate? Or does it not matter?




That's precisely what I am wondering about every time I do isolations on agar. There are visible traits which are genetic. Check my signature if you want to see an example. But I don't know whether those traits indicate health or are just arbitrary.


--------------------
:nyan: Log for my 2nd Grow (with pix and chronology)

To shut up GIFs in Firefox: Go to about:config and set 'image.animation_mode' to 'none'. Then restart Firefox.
For any other browser (especially Chrome): Uninstall browser. Switch to Firefox. See above.

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OfflineBrownBear
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Re: Post Your Cultivation Picture of The Day [Re: funky123]
    #27060172 - 11/27/20 01:11 PM (3 years, 5 months ago)

Quote:

funky123 said:
Quote:

Iambrownbear said:
Quote:

funky123 said:
The conventional wisdom here is that rhizomorphic growth is not a genetic trait but a response to environmental conditions and hence cannot be isolated.




I'm just doing this for fun to keep agar work from becoming a boring routine. I've read that rhizo growth doesn't perform any better than tomentose growth. But that is about as much as I know. Thanks for the response. How would I go about isolating a single strain? What would be the easier type of growth to isolate? Or does it not matter?




That's precisely what I am wondering about every time I do isolations on agar. There are visible traits which are genetic. Check my signature if you want to see an example. But I don't know whether those traits indicate health or are just arbitrary.




Do you have an update on any progress? I would post in your thread but I received a warning that the thread was 59 days old since the last post. Most of the plates in one of your updates back in September looked very uniform. Is uniformity a genetic trait that I should be looking for?

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InvisibleLemgrub
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Registered: 04/02/18
Posts: 644
Re: Post Your Cultivation Picture of The Day [Re: funky123]
    #27060177 - 11/27/20 01:15 PM (3 years, 5 months ago)

Thought I germinated APE yesterday, now it looks like mold😔



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We getting that pirate bay, alien shishkebab cordycep money

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OfflineA.k.aM
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Registered: 10/27/19
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Re: Post Your Cultivation Picture of The Day [Re: Lemgrub]
    #27060272 - 11/27/20 02:12 PM (3 years, 5 months ago)

Hard to tell through the plastic but it looks like it could be myc. Spores tend to look weird but also my experience with ape has been a lot of puffy growth like that.


--------------------
LAGM2020

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Invisiblefunky123
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Registered: 08/27/17
Posts: 257
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Re: Post Your Cultivation Picture of The Day [Re: BrownBear]
    #27060314 - 11/27/20 02:36 PM (3 years, 5 months ago)

Quote:

Iambrownbear said:
Quote:

funky123 said:
Quote:

Iambrownbear said:
Quote:

funky123 said:
The conventional wisdom here is that rhizomorphic growth is not a genetic trait but a response to environmental conditions and hence cannot be isolated.




I'm just doing this for fun to keep agar work from becoming a boring routine. I've read that rhizo growth doesn't perform any better than tomentose growth. But that is about as much as I know. Thanks for the response. How would I go about isolating a single strain? What would be the easier type of growth to isolate? Or does it not matter?




That's precisely what I am wondering about every time I do isolations on agar. There are visible traits which are genetic. Check my signature if you want to see an example. But I don't know whether those traits indicate health or are just arbitrary.




Do you have an update on any progress? I would post in your thread but I received a warning that the thread was 59 days old since the last post. Most of the plates in one of your updates back in September looked very uniform. Is uniformity a genetic trait that I should be looking for?




I'm updating the three main posts (setup, timeline, pictures) on a regular basis. So, next time I can just use that as recipe and improve on some details. So, actually my last update was few days ago. The tubs are slowly colonizing. No sign of contamination - I guess I'll see the first flush next week.

No idea if uniformity is a genetic trait. But I isolate as if it was - meaning, I select uniform looking mycelium for isolation and inoculation. What I found very surprising is that dishes 1 to 4 looked very nice (uniform, bit rhizomorph) in the beginning. So, I was already quite sure I'd select those for the inoculation. But after a while the development did a 180 and 1 to 4 turned into weird messy shapes while the others developed quite nicely. At some point 1 to 4 developed (all) small amber droplets while the left over dishes 7 and 9 didn't. Given that 1 to 4 were isolated from a visually different part of the initial mycelium versus the others I assume the development must have been genetic because chance would be very unlikely.



--------------------
:nyan: Log for my 2nd Grow (with pix and chronology)

To shut up GIFs in Firefox: Go to about:config and set 'image.animation_mode' to 'none'. Then restart Firefox.
For any other browser (especially Chrome): Uninstall browser. Switch to Firefox. See above.

Edited by funky123 (11/27/20 02:38 PM)

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OfflineA.k.aM
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Re: Post Your Cultivation Picture of The Day [Re: funky123] * 4
    #27060335 - 11/27/20 02:45 PM (3 years, 5 months ago)

Some top notch agar storage.



--------------------
LAGM2020

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OfflineB21
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Re: Post Your Cultivation Picture of The Day [Re: Lemgrub] * 2
    #27060340 - 11/27/20 02:47 PM (3 years, 5 months ago)


Albinos looking cool

Melmac agar pin clone

Transkei tub puts out sporeless fruits neato

11 month old atl7


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