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OfflineGermanShaman
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How does sound as a TEK?
    #27054863 - 11/24/20 04:50 AM (3 months, 11 days ago)

Spore Print to Agar to LC to several Grain spawn jars to Bulk Substrate.

I have had a lot of issues going from spore syringes to Grain. Either I get contam in the grain or contam going to bulk.

Would the above TEK also enable me to multiply my syringe volume with clean myc?


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OfflineHaywire
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Re: How does sound as a TEK? [Re: GermanShaman]
    #27055425 - 11/24/20 02:04 PM (3 months, 11 days ago)

it is difficult to obtain a 100% clean spore syringe which is probably why you're getting contaminated grains.
you're better off going spore syringe to agar, like you proposed.
If you're having issues with contamination, I would keep the LC for another grow.

go spore print to agar to grain for a couple of grows. you could even try some grain to grain.
LC is a blessing but takes a while to get the hang of it and can be easily fucked up.
to not get demotivated, I would stick to agar to grain for now.
clone some fruits, take some prints, etc.

don't mix spore syringes with liquid syringes.

good luck!


--------------------
Ciao mamma, guarda come mi diverto


My grows :mushroom2: Outdoor patches


Edited by Haywire (11/24/20 02:04 PM)


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OfflineGermanShaman
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Re: How does sound as a TEK? [Re: Haywire]
    #27055907 - 11/24/20 07:39 PM (3 months, 11 days ago)

I'm going spore to syringe to agar for now with my own or purchased spore prints.

i have a few syringes from a time before I came here so I will see if there is anything in there that can be salvaged. Lesson learnt I suppose.

Surely if I have a good agar culture I should end up with a clean LC.


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OfflineHaywire
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Re: How does sound as a TEK? [Re: GermanShaman]
    #27056465 - 11/25/20 02:28 AM (3 months, 10 days ago)

beats me why you'd want to include an unnecessary step in your progress.
good luck! I hope you succeed!


--------------------
Ciao mamma, guarda come mi diverto


My grows :mushroom2: Outdoor patches


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OfflineCocaineBuffet
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Re: How does sound as a TEK? [Re: Haywire]
    #27056477 - 11/25/20 02:41 AM (3 months, 10 days ago)

Not sure why you are so fixated on the LC when it sounds like you havent even done agar work.

You are trying to run before you can crawl.


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OfflineGermanShaman
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Re: How does sound as a TEK? [Re: Haywire]
    #27056550 - 11/25/20 05:38 AM (3 months, 10 days ago)

which one is the nu-neccessary step?  Is it from spore print to syringe?

The reason for this is to spread out the spore print. ie from one print I can make a syringe and then inoculate a lot more agar dishes and hopefully at least one or two will be successful.  Hope that makes sense.


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OfflineGermanShaman
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Re: How does sound as a TEK? [Re: CocaineBuffet]
    #27056551 - 11/25/20 05:42 AM (3 months, 10 days ago)

I have done some agar work this year and am now stepping it up to increase the quantity of my inoculate. So I can turn one or two nice agar plates into a lot more culture with which I can then inoculate even more grain.

I have not have limited success with G-G transfers.  But going G-G-G has not been successful.

So I am looking for a way to make my inoculate go further.


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OfflineHaywire
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Re: How does sound as a TEK? [Re: GermanShaman]
    #27056580 - 11/25/20 07:06 AM (3 months, 10 days ago)

spore print to syringe is unnecessary.
just make a bunch of plates and inoculate them with your print.

with one agar dish you can inoculate 5-6 grain jars. it will take a little bit longer to colonize but that shouldn't be an issue. then you can skip the G2G all together.

you can then try some liquid culture on the side.

good luck.


--------------------
Ciao mamma, guarda come mi diverto


My grows :mushroom2: Outdoor patches


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OfflineGermanShaman
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Re: How does sound as a TEK? [Re: Haywire]
    #27058047 - 11/26/20 03:37 AM (3 months, 9 days ago)

That makes sense. If go to a syringe, I can make the print go a lot further because I end up using too many spores going direct to agar. With a syringe I just use a single drop per agar dish. so instead of 5 agar dishes, I can produce 20 or 30.

All this would be done on the same time.

would that not be logical and more effcient?


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InvisibleJim Nemo
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Re: How does sound as a TEK? [Re: GermanShaman]
    #27058078 - 11/26/20 04:27 AM (3 months, 9 days ago)

you need to find YOUR individual way of growing. whatever YOU like the most, just do it.

but!
for the beginning it is important to not have to many unnecessary steps because it reduces contamn risk=contamns=frustrastion.

there is no need to make 30 agar dishes, so there is no need to save up spores from the print, so there is no need to make a sporesyringe, SO you can just put the spores from the print to the agar.

when you have a sucessfull grow, you can make like 30 new prints from one casing.

if you think: but i want to make like 50 jars of grain, how do i inoculate them with just 3-5 agar dishes? well, just start with making 5 jars, inoculate them, let them colonize and use them for grain-to-grain-transfer.

just don't complicate it to much, use time and patience as your allies and it will turn out good.
you will have plenty of opportunity to complicate it when growing panaeolus ssp. :wink:


--------------------
When the acid trip is over, you gotta come back to mother blues...


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OfflineHaywire
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Re: How does sound as a TEK? [Re: GermanShaman] * 1
    #27058114 - 11/26/20 05:53 AM (3 months, 9 days ago)

Quote:

GermanShaman said:
That makes sense. If go to a syringe, I can make the print go a lot further because I end up using too many spores going direct to agar. With a syringe I just use a single drop per agar dish. so instead of 5 agar dishes, I can produce 20 or 30.

All this would be done on the same time.

would that not be logical and more effcient?




there's millions of spores on a single print. I don't really see how you have to take it further than that.
If you take an inoculation loop and lightly swipe the print, you can inoculate 2-3 agar dishes with the spore load.
and then still have millions of spores on your print.

I just wanted to warn you that including unnecessary steps in your production process can lead to contamination and eventually demotivation.
it's like cocainebuffet said, it seems you're trying to run before you can walk.
you do you, shaman.

Good luck.


--------------------
Ciao mamma, guarda come mi diverto


My grows :mushroom2: Outdoor patches


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OfflineAdrenochrome
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Registered: 04/12/20
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Re: How does sound as a TEK? [Re: Haywire]
    #27059337 - 11/26/20 11:42 PM (3 months, 9 days ago)

There will inevitably be a period of trial and error, no matter how much you have studied ahead of time. The mushroom must teach you through experience from start to finish.  :fuckthisshit:


--------------------
There is a pleasure in the pathless woods,
There is a rapture on the lonely shore,
There is society, where none intrudes,
By the deep Sea, and music in its roar:
I love not Man the less, but Nature more,
From these our interviews, in which I steal
From all I may be, or have been before,
To mingle with the Universe, and feel
What I can ne’er express, yet cannot all conceal.
Lord Byron


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Invisiblebigfootscreepyuncl
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Re: How does sound as a TEK? [Re: Adrenochrome]
    #27059416 - 11/27/20 12:58 AM (3 months, 8 days ago)

I don't want to hijack a thread but it seems somewhat relevant. I just ordered my very first ever syringes (5, in fact..a bit ambitious but I wanted to minimize the number of shipments) and my plan is as follows:

Start with PF TEK (PC, BRF cakes)
Colonize
Spawn to bulk in shoeboxes and unmodified monotub
Harvest
Clone to agar and collect prints to begin practicing agar work

Does this sound like a good progression for a total noob?

Cheers, mates!


P.S. Sorry to hijack a thread, yell at me if you must!


--------------------
Bigfoots LAGM21


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OfflineHaywire
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Re: How does sound as a TEK? [Re: bigfootscreepyuncl]
    #27059525 - 11/27/20 02:47 AM (3 months, 8 days ago)

yes, you can do that.


--------------------
Ciao mamma, guarda come mi diverto


My grows :mushroom2: Outdoor patches


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OfflineTattersail
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Re: How does sound as a TEK? [Re: Haywire]
    #27059605 - 11/27/20 06:01 AM (3 months, 8 days ago)

The only time I've bothered making spore syringes lately is when a friend wanted to give BRF jars a go, so I made him a couple. For doing agar work, making a syringe is an unnecessary step - a teeny tiny scrape of spores is all you need to start some plates, and the spore print can be used a bunch of times if it's kept clean enough. As others have said, you really don't need 30 spore plates, like 2-5 is plenty. Once you get clean growth and a a few transfers, you can make dozens of clean plates without having to go back to the spores. And once you get fruits, you can make your own fresh spore prints, as many as you like.


--------------------
LAGM2021
Trades

We may lose or we may win, but we'll never be here again


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