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OfflineKavalandiaShamasha
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Registered: 09/24/20
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Pinning before substrate is fully cultivated?
    #27059401 - 11/26/20 10:40 PM (3 years, 2 months ago)

Hello,

I used a grain spawn bag that I inoculated about 2 months ago. I'm glad I didn't ditch it or give up on it because after a few weeks, all I could notice was what looked some cloudy boogers in the bag and then a few black dots I didn't remember being there that I thought must have been contamination. But when I checked it again recently, I was very happy to see a majority of the substrate covered in white. However, I noticed today that a few pins have already started coming up but a portion of the substrate is still not yet colonized. Is this abnormal and could it potentially cause issues? I have read early pinning could be because of contamination and I am not sure what to do if that is the case. Is it too late to mash the bag around some in case the substrate is just too caked together? Also, what can I do with the substrate after I harvest?

If you have any tips on what to do going forward, I would love to hear them. It has been a while since I brushed up and I have a rough idea of what I'll need to do but I always like hearing the varying tips and techniques people discover.

Thanks and much love

Edit: I wanted to add pictures but for some reason it isn't allowing my .jpgs


Edited by KavalandiaShamasha (11/26/20 10:44 PM)


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Invisiblesh4d0ws
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Registered: 02/26/08
Posts: 12,086
Re: Pinning before substrate is fully cultivated? [Re: KavalandiaShamasha]
    #27059406 - 11/26/20 10:46 PM (3 years, 2 months ago)

Shroomery won't allow jpegs? Could just be the upload system being fucky. Trying uploading them to imgur or something if you're comfortable, or open it in MS paint and save it as a different file type and try that

:shrug2:


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OfflineKKVguitarist
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Re: Pinning before substrate is fully cultivated? [Re: sh4d0ws]
    #27059421 - 11/26/20 11:03 PM (3 years, 2 months ago)

Pinning before full.colonization can happen. With some varieties, if it starts jist crumble the bag up and shake it up. Dont let those pins get any bigger try to do it as soon as.possible


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OfflineKKVguitarist
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Re: Pinning before substrate is fully cultivated? [Re: KKVguitarist]
    #27059424 - 11/26/20 11:05 PM (3 years, 2 months ago)

It can also be a sign of contamination SOMETIMES but that doesn't mean your is contaminated I have an especially frisky set of.creeper generics that when the growth was only halfway thru the petri dish started pinning it was the craziest shit I ever saw. I cloned the first pin that formed and im.growong both the cleaned up m.s. genetic and the cloned hyphal know genetics so well see what happens!!

But when I've seen premature pins i just break up my substrate really.good and shale.it all up it will get the substrate fully colonized by the time.it starts pinning again


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OfflineKavalandiaShamasha
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Re: Pinning before substrate is fully cultivated? [Re: KKVguitarist]
    #27059492 - 11/27/20 12:30 AM (3 years, 2 months ago)

Thank you for the info. I thought mashing it would help but I wasn't sure if it was okay because I had heard something about potentially damaging the mycelium. I will go ahead and do that now and I am working on uploading the pics to imgur


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OfflineKavalandiaShamasha
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Re: Pinning before substrate is fully cultivated? [Re: KavalandiaShamasha]
    #27059522 - 11/27/20 12:40 AM (3 years, 2 months ago)

Alrighty, here are some pictures. For some reason my imgur app is crazy slow. Also, I went ahead and made them public to make sure you can see them but if I post them privately, can people still see them if I share the link?

1 https://imgur.com/gallery/Gr3hdSg


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OfflineKKVguitarist
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Re: Pinning before substrate is fully cultivated? [Re: KavalandiaShamasha]
    #27059523 - 11/27/20 12:45 AM (3 years, 2 months ago)

Damaging the mycelium? No, literally all grow methods except pf tek really has a break and shake step.

If you haven't broken this mycelium up at all then you've been playing the suuuuuper long game.

Once that grain was pike 30% colonized if you break and shake it will speed colonization ALOOOOOOOT. And that explains why you got early pins, the food source in the middle of the mycelium.was fully consumed so it goes into the next phase of growing.


Now this is MY opinion, but I break and shake a.minimum of 2 times.

Once at 30% and if it gets spread around really well ill do it 2 more times around
80% and again once its fully colonozed.


After its fully colonized I break.ot up very very well and shake good then I allow it to fully recover into a solid block this guarantees every tiny bit of available nutrition is co sumer

Your doing grain so after that you will crumble it 1 more time and distribute it into your substrate bags.

With my all in 1 bags I just press the substrate flat into the botto. Of the bag to give the mushroo.s a nice flat top surface to grow from and I only open the bag when it's tome.to harvest


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OfflineKavalandiaShamasha
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Re: Pinning before substrate is fully cultivated? [Re: KavalandiaShamasha]
    #27059528 - 11/27/20 12:48 AM (3 years, 2 months ago)

Did I mash it too much? It was a bit caked together but it would appear not very much of it was colonized at all, just the outside. I gave it a good shaking and tried to make sure all the pins were covered up in case that matters. I also tried to leave a small section in tact but should I make sure it is all broken up in case the inside isn't colonized? I could actually smell the mushroom though while I was shaking it. And yeah this is my first grow if you can't tell.

I'm also curious about how the grain that obviously wasn't colonized being the original yellow color but the parts that had a good bit of mycelium are brown. Is that from the nutrients getting sucked out?

2 https://imgur.com/gallery/l4iQfph


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OfflineKKVguitarist
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Re: Pinning before substrate is fully cultivated? [Re: KKVguitarist]
    #27059530 - 11/27/20 12:49 AM (3 years, 2 months ago)

Yeah that looks.lile it might have some bacteria in there.


When you break it up and shake it you'll find out. Where theres bacteria mycelium has a hard time.colonizong this is why people.dont suggest innoculating grain with spore syringes. If that was a Patek cake you could birth it even with that bacteria, wash the bacteria part off and get mushrooms.from the rest of the healthy growth.

But because thats  grain when you shake it up the bacteria is going to get everywhere and it won't recover.

That she be recovered fuzzy and reconnecting in 2 or 3 days.

That looks almost fully colonized so it should recover in 1-2 days.

If it goes 3+ days and doesn't recover you need to start over dont waste you time and supplies trying to spawn with bacterial spawn. Its just gonna contaminate


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OfflineKKVguitarist
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Re: Pinning before substrate is fully cultivated? [Re: KKVguitarist]
    #27059531 - 11/27/20 12:52 AM (3 years, 2 months ago)

Yeah the way that theres no condensation but theres specific wet different covered grains are bacterial contamination. Very common with spore syringes.


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OfflineKavalandiaShamasha
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Re: Pinning before substrate is fully cultivated? [Re: KKVguitarist]
    #27059533 - 11/27/20 12:52 AM (3 years, 2 months ago)

I see. I had planned to shake a couple times and I did at least once but after a month of 0 progress, I figured it was a dud and forgot about it until recently. I have some pasteurized mystery stuff bags (brownish/black stuff, it didn't say online what it was) but I'm not sure if they're still good as I bought them over 2 months ago. My plan was kind of just to let these sprout in the bag and then harvest them when the veis come off; can I not do that?


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OfflineKavalandiaShamasha
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Re: Pinning before substrate is fully cultivated? [Re: KKVguitarist]
    #27059538 - 11/27/20 12:55 AM (3 years, 2 months ago)

Thank you for the info. I did what I could to ensure sterile inoculation (alcohol wipes, gloves, still air--I didn't flame the tip of the needle because it was brand new in a sealed package but I will do that next time just in case).

So it should potentially still work? Assuming it grows, does bacteria effect any of the rest of the process or they'll still be safe but maybe not as fruitful as they could have been?


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