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OfflineBanjoFox
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Registered: 11/10/19
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Loc: USA
Last seen: 1 year, 9 months
Agar - Identification help appreciated
    #27057977 - 11/25/20 11:47 PM (3 years, 2 months ago)

Hello :laugh:

I am just getting started with starting spores on agar, and am trying to identify what's currently growing.  Having searched the forums for other photos, I have not yet found anything that looks like what I have currently.

The plates I have came from a reputable dealer (my PC is in the mail :wink:), and the spores came from a sponsored vendor.  So far the Dancing Tiger plate patches of cotton ball like tufts growing from it.  They're a pretty pure white, and I read that white molds look "dirty" so... I'm up in the air on that one

The PF Redspore plate has sprawling, yellowish colonies which have a shine to them.  There's also a few shiny white colonies, one of which I swabbed onto a third plate (it was sufficiently isolated from everything else).

Help is appreciated :smile:

Dancing Tiger - November 17, 2020


PF Redspore - November 17, 2020


PF Respore - Isolated on November 23, 2020 from the Nov 17 plate


Edited by BanjoFox (11/27/20 07:13 AM)


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OfflineGroo
sola dosis facit venenum
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Registered: 12/06/14
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Re: Agar - Identification help appreciated [Re: BanjoFox]
    #27057991 - 11/26/20 12:13 AM (3 years, 2 months ago)

It seems you are growing death, death and more death.

Mold

Bacteria

More bacteria.


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OfflineGroo
sola dosis facit venenum
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Re: Agar - Identification help appreciated [Re: Groo]
    #27057994 - 11/26/20 12:15 AM (3 years, 2 months ago)

Your technique seems good i think someone sneezed directly on your spore print. Then if fell on the floor. Perhaps if you had some Antibiotic plates you may be able to take those spores to agar. Probably should have done transfers every 3 days for 3 or 4 times. you gotta clean them up.


Edited by Groo (11/26/20 12:18 AM)


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Offlinescarabaeus
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Registered: 06/13/18
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Re: Agar - Identification help appreciated [Re: BanjoFox]
    #27058024 - 11/26/20 12:57 AM (3 years, 2 months ago)

I think it is against Shroomery rules to mention non-Shroomery sponsored companies in your posts. There is an 'edit' function you can use to remove said violations.

Those plates are a nightmare.  Do more research, try again, and then there will be many peeps here that will  be happy to help you move you along with your project. My advice is always "screw the internet", get your growing info here.


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Offlineblack strat
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Registered: 10/31/20
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Re: Agar - Identification help appreciated [Re: scarabaeus]
    #27058038 - 11/26/20 01:17 AM (3 years, 2 months ago)

edit: complaining isn't attractive.


Edited by black strat (11/27/20 05:52 PM)


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Offlinescarabaeus
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Re: Agar - Identification help appreciated [Re: black strat]
    #27058077 - 11/26/20 02:25 AM (3 years, 2 months ago)

q




Edited by scarabaeus (11/26/20 03:06 AM)


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OfflineBanjoFox
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Re: Agar - Identification help appreciated [Re: scarabaeus]
    #27059683 - 11/27/20 06:46 AM (3 years, 2 months ago)

Original post edited to remove the non-sponsored vendor (mah bad).

This is pretty much the only place I actually trust for growing advice.  It sucks that I probably gotta toss out the syringes though.  The plates... fine who cares I have seven other clean ones.  But since I didn't see ANY colonies that looked remotely like the ones pasted all over the forum I'm not super confident in the syringes any more.

Yes I do know that syringes are not super clean, but the plates were sold as malt extract + 0.01% chloramphenicol for bacterial prevention.

I DO have clean homemade BRF jars I could try just for the laugh.  This is the first time I've done spores on agar (I used to play with catching wild yeast for brewing projects).  At least with BRF I was successful one time so I should be able to identify problems with that a lot quicker haha.


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OfflineGroo
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Re: Agar - Identification help appreciated [Re: BanjoFox]
    #27059919 - 11/27/20 10:12 AM (3 years, 2 months ago)

I'm on the cell phone so I'm not sure if we've already discussed Gentamicin antibacterial agar and learn how to pull your own plates and saving those syringes don't toss them bro learn to grow them out DBB you got this.


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Offlineblack strat
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Registered: 10/31/20
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Re: Agar - Identification help appreciated [Re: Groo]
    #27060613 - 11/27/20 05:50 PM (3 years, 2 months ago)

yea, to be clear, I didn't toss my syringes either. a few people here encouraged me to jus use them on BRF cake attempts, which it sounds like you're planning on the same.

I made about 13 4oz and 8oz cakes the other night. when I finally took em out the PC, the dry verm layer was wet. devastating. so so so disheartening. I guess I gotta jus keep trying tho, yeah?  lol i dunno.


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OfflineShroomoisseur
Professional Cucker

Registered: 11/16/20
Posts: 138
Last seen: 11 months, 15 days
Re: Agar - Identification help appreciated [Re: black strat]
    #27060693 - 11/27/20 07:07 PM (3 years, 2 months ago)

If you haven’t gotten petris yet to pour your own I would follow pasty no-pour tek! Just PC them and they’re ready to go, I’m already seeing growth on some plates. Also don’t put too much solution on a plate, 1 or 2 drops per. Sorry those were contams!


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Offlinescarabaeus
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Re: Agar - Identification help appreciated [Re: black strat]
    #27065613 - 11/30/20 11:10 PM (3 years, 1 month ago)

RE: black strat. You are right on -complaining isn't attractive. The post you are referring to was intended for a different web site where someone was being a troll. I accidently cross referenced my file and posted here rather than in my target which is why it was immediately removed -oops!  :facepalm3: I feel bad about it even though it was only up for a minute or two. But sometimes people need a good ass reaming (but it was not anyone here) IMO. Hope this clears things up if we are talking about the same thing. :thumbup:

Are we talking about the same thing? Or did you just misidentify me in your response post?  Doesn't matter lol. Keep rockin' it.


Edited by scarabaeus (12/01/20 12:08 AM)


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OfflineBanjoFox
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Registered: 11/10/19
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Re: Agar - Identification help appreciated [Re: Shroomoisseur]
    #27071910 - 12/04/20 01:29 PM (3 years, 1 month ago)

Quote:

Shroomoisseur said:
If you haven’t gotten petris yet to pour your own I would follow pasty no-pour tek! Just PC them and they’re ready to go, I’m already seeing growth on some plates. Also don’t put too much solution on a plate, 1 or 2 drops per. Sorry those were contams!




Yeah I have Pyrex dishes, and a brand-spankin' new pressure cooker to use :smile:

Because I didn't have the PC at the time, and because I'm a lazy scientist I bought 10 of the green plates, which also have 0.01% chloramphenicol as a bacterial inhibitor.  I've actually upgraded my agar working area, and tek to try -one- more time from the syringes, and I'm seeing the same white fuzz, but this time I also see grainy colonies, which were not apparent in the ones here (posted new photo in the contams thread).

Right now the *biggest* issue that I have with BRF is that I'm actually having a hard time finding the right brown rice.  None of whats in stores near me has the hulls.  Even anything labeled "organic".  Searching the forum a bunch I actually found very little info on specific brands, or "here is what the brown rice should look like".  It's probably there, but using BRF or brown rice brings up a LOOOOOOOOOT of posts.


I did make brown rice flour in my coffee grinder with the rice I had in my kitchen, but only BOB knows if its actually gonna work :smile:


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