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Ackso
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Pc times, help with my grow
#27057884 - 11/25/20 10:11 PM (3 years, 2 months ago) |
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Hello everyone! Been reading on here for a few months and had a successful (ish) first grow. Ever since then, its been a catastrophe 😞. Im finally asking for some help and think I have narrowed it down, however would still like to describe my process in case you guys can point out any other obvious flaws. This may be a bit long, but I know people are going to ask specifics, so bear with me.. Or scroll to bottom for main question
Grain prep: Organic Rye Berries soak with coffee and gypsum24H boiled 5-10 min, dried, bagged Substrate prep:50% organic steer poo (couldnt find horse) 25%coir 25%verm+gypsum, pastuerize Lights: 6500k 12/12 cycle Pc: 14 litre @15 psi Pastuerizing: steam in myco bags in 15 gallon stockpot with raised perferated bottom until temp reaches 140F, let free rise to 160F
First grow! Golden teacher MS syringe> 1.5lb rye bag> PC 1h 45 min roughly 4 week colonizing time> spawn to monotub. Now unfortunatley my grain to sub ratio was very low (aprox 1:8) so yield was poor..but they still worked!
For my next grow I use Huautla LC> 1.5lb rye bags, PC 1h 45 min, colonized within 4 weeks again, smells like fresh mushrooms too..so far so good! New grain to sub ratio 1:3 btw. Now This is where things fall apart: my thermometer malfunctioned when pastuerizing substrate..not realizing this till weeks later when a working secondary thermometer read 40 degreeF higher! These monos are giving me a decent first flush but then white mold, metabolites flies and trich is starting to appear. On subsequent monos of the same thing the trich is showing up before I can even harvest a first flush.
At this point im thinking "okay the sub is under pastuerized"
Around this time I switch to 3lb grain bags (huatla still) @ 4 bags per pc run..but dont adjust the PC time, i start noticing some of these bags contaminate. Bump up my pc time to 2h 15min. inoculate these new bags with b+, no mycelium growing and filter patch smells like cat pee(no i dont own cats). Convinced it was the B+ lc I prepped more grain bags(3lb @2h 15 min) inoculate with Transkei...cat pee again. Now im realizing its my pc sterilizing thats the issue so my question is this!:
How many lbs per bag, how many bags per pc? Ive read so much about pressure vs time but nothing definitive on grain mass and pc size. Just ordered a presto 23 Q. Doing 1.5lb x 8 bags in a pc seemed to work for me, but id like to be a little more effecient, ya know? Bags arnt that cheap... Other than the pc questions am I missing something?
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Sir Pentinite
Stranger all the time.

Registered: 05/15/19
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Re: Pc times, help with my grow [Re: Ackso]
#27057896 - 11/25/20 10:25 PM (3 years, 2 months ago) |
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Quote:
Ackso said:
First grow! Golden teacher MS syringe> 1.5lb rye bag>
For my next grow I use Huautla LC> 1.5lb rye bags,
How was the LC prepared? Did you test it on agar first?
Have you done any uninoculated control bags to see if it's actually the PC cycle?
-------------------- "I thought to myself 'Boy, I'm sure glad there's nobody here to see this because this is exactly the sort of thing that gets people riled-up and they assume you're dying and that something has to be done. Where if you're alone, you know, you either come through it or you die, but in any case you avoid the fuss.'" - Terrence McKenna
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Ackso
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Huautla lc was purchased from someone and no I didnt test on agar, great point! Although.. They looked and smelled good. Also I tested the b+ and transkei on Agar..looks clean! Also no control bags ..how would one do this??
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MH5109
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Re: Pc times, help with my grow [Re: Ackso]
#27057905 - 11/25/20 10:35 PM (3 years, 2 months ago) |
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Pc a control bag and let it sit.
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Ackso
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Re: Pc times, help with my grow [Re: MH5109]
#27057931 - 11/25/20 10:57 PM (3 years, 2 months ago) |
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Good call, but if 1.5 lb bags worked and the LCs are clean on agar, thats really the only other thing that could be it..?
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MH5109
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Re: Pc times, help with my grow [Re: Ackso]
#27057949 - 11/25/20 11:09 PM (3 years, 2 months ago) |
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If everything worked then you don't have anything more to test. But if you are uncertain about the bags being completely sterile after the pc cycle then a test bag would be another avenue to test.
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Ackso
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Re: Pc times, help with my grow [Re: MH5109]
#27059211 - 11/26/20 07:42 PM (3 years, 2 months ago) |
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What is the best method then, for configuration of my bags in the pc? Weight? Time?
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Sir Pentinite
Stranger all the time.

Registered: 05/15/19
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Re: Pc times, help with my grow [Re: Ackso]
#27069899 - 12/03/20 11:36 AM (3 years, 1 month ago) |
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Quote:
Ackso said: Huautla lc was purchased from someone and no I didnt test on agar,
Are you talking about liquid culture or spore solution? It's not clear to me and they are very different.
-------------------- "I thought to myself 'Boy, I'm sure glad there's nobody here to see this because this is exactly the sort of thing that gets people riled-up and they assume you're dying and that something has to be done. Where if you're alone, you know, you either come through it or you die, but in any case you avoid the fuss.'" - Terrence McKenna
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PBJ710
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Re: Pc times, help with my grow [Re: Ackso]
#27070072 - 12/03/20 01:24 PM (3 years, 1 month ago) |
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Quote:
Ackso said: What is the best method then, for configuration of my bags in the pc? Weight? Time?
There's really not alot of magic to packing a PC with bags - just make sure they're not directly touching the bottom especially if you have a gas stove. If the bags are not presealed, you will need to make sure the opening is not below the water level. Turn on the PC and after it starts making steam, let it vent for 10 minutes then add the weight and bring it up to pressure and start your timer. I PC 4lb bags for 3h at 15psi or 2h at 20psi.
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polaritymind
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Re: Pc times, help with my grow [Re: PBJ710]
#27070153 - 12/03/20 02:16 PM (3 years, 1 month ago) |
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I personally, not having a HEPA and only a SAB dont fuck with LCs anymore, cost me too many nerves. Annoying thing is you cant see the contam. If youre gonna use bags (and I really dont get the point, seems unnecessary plastic and waste production to me) why not inoculate them with grain spawn? But yeah, since I know nothing about these bags I cant really tell, but the filter or any other spot might be the weak spot. Less chance of that with jars and well-prepared lids, not hard to make them either. I just use a screwdriver and polyfill, pack as tight as possible. Also sterilized hpoo likes to contam if there is air contact, or generally hpoo costs nerves, coir straight (maybe some gypsum) performs just as well if not better.
-------------------- "to affirm life is to also affirm death" -Albert hofmann
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Ackso
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Its LC yeah. And thanks for all the replies guys! Much appreciated!
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Professor X
School for the Gifted



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Re: Pc times, help with my grow [Re: Ackso]
#27134331 - 01/08/21 12:45 AM (3 years, 20 days ago) |
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I don't see the point in LC or bags. I do agar and jars. 95% success rate with a glowhood made from a febreeze hepa purifier, a small fan, a tub, some tubing and a trash bag. Do all my work a few feet from 2 cages housing 7 male rats. When I do get a bad jar it's usually because someone walked in the room during that transfer. Cubes are super easy to grow, if you sterilize properly and use proper technique it's very easy. I like simple when it comes to mush cult. 40 minute wbs runs and 2hr pc times. I never have an unopened jar fail.
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