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madyogi
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Re: In Over My Head Agar -A Novice Agar Adventure [Re: c10h12n2o]
#27086237 - 12/12/20 06:27 PM (3 years, 1 month ago) |
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The APE plate really looks like a wookiee, or maybe a wise old owl ... if you tilt your head right
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Ababyphoenix
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Re: In Over My Head Agar -A Novice Agar Adventure [Re: c10h12n2o]
#27095574 - 12/18/20 09:59 AM (3 years, 1 month ago) |
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Massive update on all my strains here. All the transfers (30+) i did don't have a single contam (getting that sterile technique down!) so I'm officially ready to start thinking about grain but I'm about to have 17 shoeboxes from MSS running so I'm not too hurried. I apologize for the image quality. I will do Medium next time this is the first time I did SMall
RW the first pic is suspect to me. It might very well be white mold. All my RW plates look... interesting

LPEU (Dish Pin)

AA+ Im honestly disappointed with these T.4's...

TC Same with these t.4s

Mel

And my girlfriend got me a cool tapestry for the grow room 

Ill sift these into their categories sometime today but that's all for now. Im between Mel LPEU and AA+ being my favorite looking culture here. Im thinking Mel. Cant wait to see what it grows out!!
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   Pho's MushiAventures:
My '21 GLOG LAGM 2022
Edited by Ababyphoenix (12/18/20 10:18 AM)
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c10h12n2o
serial dilutor



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Re: In Over My Head Agar -A Novice Agar Adventure [Re: Ababyphoenix] 1
#27095811 - 12/18/20 12:55 PM (3 years, 1 month ago) |
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good job!! looks like you are taking to agar like a champ 
+5'd ya for your persistence
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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Ababyphoenix
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Re: In Over My Head Agar -A Novice Agar Adventure [Re: c10h12n2o]
#27095855 - 12/18/20 01:37 PM (3 years, 1 month ago) |
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Thanks c10!
 Really means a lot to hear from you Soon I'm going to have to make my mailman work for his money as well
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   Pho's MushiAventures:
My '21 GLOG LAGM 2022
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DrunkUncle


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Re: In Over My Head Agar -A Novice Agar Adventure [Re: Ababyphoenix] 1
#27096137 - 12/18/20 04:29 PM (3 years, 1 month ago) |
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lookin great homie!
-------------------- OEF Combat Medic & Purple Heart Recipient “If you get the message, hang up the phone. For psychedelic drugs are simply instruments, like microscopes, telescopes, and telephones. The biologist does not sit with eye permanently glued to the microscope, he goes away and works on what he has seen.” ― Alan Watts Smartattack said: I was doing Tai chi while flying through space, took a shit in my shower, removed my face several times with my hands and that was all just the boring part before it really hit me.
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madyogi
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Re: In Over My Head Agar -A Novice Agar Adventure [Re: DrunkUncle]
#27102139 - 12/22/20 08:05 AM (3 years, 1 month ago) |
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Nice going. The tapestry is badass too!
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Ababyphoenix
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Re: In Over My Head Agar -A Novice Agar Adventure [Re: Ababyphoenix]
#27117921 - 12/31/20 10:06 AM (3 years, 28 days ago) |
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Sorry for the silence on this thread, the holidays and everything. Anyways here's an update on the dishes 
I purged every plate that had a contam and have a healthy mountain of clean dishes to continue working with and many that look fully ready for grains.
(Note the intuitive use of a mason jar and a straw cut in half... When u drive off with ur e-rig on ur roof... lets not talk abt it)
Here are the 8 plates ill be putting on grain shortly after this update. Probably 3-4 jars a plate.

4x Mel - this culture was a stong colonizer so i will do 4 jars with one plate 2x AA+ - This culute compared to the others looks weaker than the Mel in terms of obvious myc growth but i have a feeling it will fruit well 2x LPEU - Im chalking the weird look of these plates to the fact that its PE cause this is about 4 transfers to a pin then 2 transfers from that dish pin so I'm assuming given the weird look these cultures are still clean.
A side note many MANY of my dishes are pinning so I need to work with these more recent transfers NOW before they all start pinning as well lol
Meanwhile im harvesting/casing these staggard MSS shoeboxes as they grow getting my masons back in the game.. as well as.. I'm going to buy more right now never have enough masons


Mush love, and updating the main post here shortly.
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   Pho's MushiAventures:
My '21 GLOG LAGM 2022
Edited by Ababyphoenix (12/31/20 10:09 AM)
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roarkell
The Roar Master!



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Re: In Over My Head Agar -A Novice Agar Adventure [Re: Ababyphoenix] 1
#27121652 - 01/02/21 07:38 AM (3 years, 26 days ago) |
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Hell yeah dude! Looks like you got your agar game locked down! Excited to see what you get outta all the hard work you already put in!
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Ababyphoenix
You



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Re: In Over My Head Agar -A Novice Agar Adventure [Re: roarkell]
#27130403 - 01/06/21 01:08 PM (3 years, 22 days ago) |
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APE, R.Mel, AA+, TC and LPEU have all been put to grains. I feel like this years getting better by the hour 

Should have some fruits in a month or so  I have monotubs in mind for mel at the least, i figured its time i up my size. Also looking into muds buckets for BE and not spawning to open air? That sounds cool. Load that sub up with gewds, but more pc time.
I want to join in on LAGM21 but im so busy this week and already in over my head with cultures. So heres my lagm2021 started a bit early lol
Im thinking about LC sure... but i want to take a clone that i know is a prolific fruiter to lc. I need to see how these jars go
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   Pho's MushiAventures:
My '21 GLOG LAGM 2022
Edited by Ababyphoenix (01/06/21 01:17 PM)
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madyogi
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Re: In Over My Head Agar -A Novice Agar Adventure [Re: Ababyphoenix] 1
#27130521 - 01/06/21 01:40 PM (3 years, 22 days ago) |
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Great work, Pheonix, can't wait to see how it all flushes out!
I've been on a similar journey, though less ambitious. I put a Mexi-Cube strain from MSS through 4 transfers to get some really nice plates and put those to grain this morning. You can see my latest update here.
I put the three best plates to two different types of grain (whole brown rice and whole oats), because I haven't been having the best of luck with cubensis on WBS lately. Brown rice has been working better for me, but I'm rooting for the whole oats, because they're hella cheap where I'm at, like 50 lbs for $15.
Onward!
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Ababyphoenix
You



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Re: In Over My Head Agar -A Novice Agar Adventure [Re: Ababyphoenix]
#27144304 - 01/12/21 06:01 PM (3 years, 16 days ago) |
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Quick little update here on some of the cultures. A lot of the plates are pinning so here's some cool shots as well as some grain.

  My APE cultures keep doing this weird split section thing between nice rhizo growth and this weird idk what stuff. At first I thought it was contamination and I'm still not sure it isn't but it looks cool. I ended up just cutting out the good looking part and putting it to grain  PE/T C+ update is huge TBH... Im gonna put some of this ish on grain
  Mel Pins BABY! Making me proud on agar hopefully they likes coir verm
 RW. These plates are questionable? This myc looks... clean but also weird and very circly if that makes sense. But I've seen other peoples petris of clean myc i trust who look like this so I think they are good cultures. Input would be cool
  
  Some good grain shots! New Sig for sure
    
And the stacks on the table 

Sidenote All my MSS shoeboxes are coming along good. Only one went green so far out of 6 so 
Anyways that's all for today. Love yall
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   Pho's MushiAventures:
My '21 GLOG LAGM 2022
Edited by Ababyphoenix (01/12/21 06:06 PM)
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Ababyphoenix
You



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Re: In Over My Head Agar -A Novice Agar Adventure [Re: Ababyphoenix]
#27155263 - 01/18/21 11:50 AM (3 years, 10 days ago) |
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Another update here. I need to step my game up to LI/LC because this whole agar to grain thing is slow as hell. Especially because my girlfriend is always hot so we keep the heat off and I haven't gone to get a space heater for them so the room stays about 64-68 and even colder at nights so colonization is considerably slow. I also did one wedge to most jars and I'm seeing the jars that got a couple wedges are colonizing faster obviously... More innoc points. So lesson learned I need to cut up the wedges into smaller pieces. I just opted for less time dropping agar into the grain and the lid being open but once I'm comfortable... Anyway.. Heres update pics, almooossst there... 17-18 days after innoc tho..
All Melmac
 Left to right AA+ then the pints are TC back to quart LPEUs
 APEs
 Probably about ready for a shakin
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   Pho's MushiAventures:
My '21 GLOG LAGM 2022
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madyogi
Stranger



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Re: In Over My Head Agar -A Novice Agar Adventure [Re: Ababyphoenix]
#27159782 - 01/20/21 03:20 PM (3 years, 8 days ago) |
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Glad to see the update, Phoenix!
It seems we're on a similar update schedule - Mexi-Cube Isolation to Sub Part 1
Looking forward to seeing results!!
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Ababyphoenix
You



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Re: In Over My Head Agar -A Novice Agar Adventure [Re: Ababyphoenix]
#27182533 - 02/02/21 09:38 AM (2 years, 11 months ago) |
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Well 30 days after inoculation all these jars are nearing the end and ready to be spawned. As suspected the R.Mel is the quickest and nicest looking culture here so she went to my first mono Wish me luck!
 Decided 5 qt myco spawn should let me sleep at night

LPEU and AA+ are very near done probably could go to shoeboxes today. The TC pint jars i want to G2G but the culture didn't wow me on agar so i dont see too much of a point other than experience. Many many more grain spawn jars being prepped as well as Muda Bottles for some of these master jars i dont trust 100%.
As for everything on agar still its all pinning like no tomorrow. I need to do some more A->A transfers to get new plate growth but for now Im starting 3 new projects that I'm very excited about. APEU, Quirckmiesters Melmac, and D3's RW x MM so a lot my attention will be going to cleaning and fruiting these cultures now that i have all these cultures almost ready to fruit (APE is almost ready to spawn but i fell of on RW). Its been a wonderful journey and ill most likely start a new log for the above mentioned adventure. Thanks for reading along everyone, ill keep posting as the tubs fruit out.
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   Pho's MushiAventures:
My '21 GLOG LAGM 2022
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Tylrsail
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Re: In Over My Head Agar -A Novice Agar Adventure [Re: Ababyphoenix]
#27204397 - 02/13/21 07:08 PM (2 years, 11 months ago) |
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Love your writing Ababyphoenix! If this mycology thing doesn't work out I think you have a solid career as a TV show writer... Good stuff
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Shakedown Street
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Re: In Over My Head Agar -A Novice Agar Adventure [Re: Tylrsail]
#27206096 - 02/14/21 06:32 PM (2 years, 11 months ago) |
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It is obvious, Ababyphoenix, that you have gotten your agar groove going well. It's been a year since I started growing mycelium on agar, after trying to grow mycelium in LC. In my most recent batch of mycelium growing on agar, I selected #1 and #2 at reaching the edge of the petri dish first, before all other contenders. So, naturally, I will make sure those two get duplicated on more than one petri dish. If you have any other pointers I would certainly listen.
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Ababyphoenix
You



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Re: In Over My Head Agar -A Novice Agar Adventure [Re: Ababyphoenix]
#27209954 - 02/16/21 06:18 PM (2 years, 11 months ago) |
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Melmac mono pinning up decently.


LPEU and AA+ are taking their time on CV Tips for you are keep at it and improve your technique until you get the results you want
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   Pho's MushiAventures:
My '21 GLOG LAGM 2022
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madyogi
Stranger



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Re: In Over My Head Agar -A Novice Agar Adventure [Re: Ababyphoenix]
#27210641 - 02/17/21 07:17 AM (2 years, 11 months ago) |
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Ababyphoenix
You



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Re: In Over My Head Agar -A Novice Agar Adventure [Re: Ababyphoenix]
#27213179 - 02/18/21 02:04 PM (2 years, 11 months ago) |
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   Pho's MushiAventures:
My '21 GLOG LAGM 2022
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Ababyphoenix
You



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Last seen: 24 days, 22 hours
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Re: In Over My Head Agar -A Novice Agar Adventure [Re: Ababyphoenix]
#27214785 - 02/19/21 12:07 PM (2 years, 11 months ago) |
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First Mono success! No melmac but not dissatisfied!




 1300g ish wet from 5 qt spawn. Not ideal but fuck yes!

Cloned the fastest biggest cluster and printed that big beaut


2/24 Shoebox 300+g wet

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   Pho's MushiAventures:
My '21 GLOG LAGM 2022
Edited by Ababyphoenix (02/24/21 02:08 PM)
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