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shadyshroomie
MycoCurious



Registered: 10/29/20
Posts: 132
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LC.....
#27057361 - 11/25/20 04:11 PM (3 years, 2 months ago) |
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Preparing for the shit storm of comments I am about to receive for this post. I get it. I do. It is okay. LC might suck, G2G may be way better, that is fantastic. Regardless, science is still science. I believe success in this hobby comes from knowing what you are doing, how you are going to do it, and most importantly why you are doing it. If I had a fat HEPA flow hood and unlimited space for spawn jars and a fat pressure cooker, then yeah, I would do that, and I would also do agar. I do not have a sterile enough environment to feel comfortable constantly exposing my cultures or grains to air.
I will be growing for probably about 6 months. It is not reasonable for me to start agar isolates from MS syringe and test all the strains. I want to create a decent clone pretty fast and create a master culture to inoculate grain spawn bags (perhaps I am missing another TEK other than LC to do this?). I want to have as sterile of a procedure I can with my resources, and no longer rely on MS syringes. I will not use another method other than injectable grain spawn bags due to several reasons (ease, no time to make massive amounts of spawn, the chance I fuck up and ruin spawn, no flow hood, etc etc).
I will be inoculating probably around 10 1 lbs. bags a week or so, maybe less, hopefully more  Using LC, how can I store a culture for the long term to serve as a master to then make necessary LC syringes however often needed to inoculate my bags. I do not want to open jars, open bags, etc. The only thing outside the LC and bag I will use is agar plates simply to test LC and check for contaminates before knocking up hella bags.
My thoughts are to clone a fruit of choice by stabbing a piece of flesh with a syringe and putting it into an LC (with sterile technique of course following the various TEKs to do so). I will have jars with LC injectable lids. I understand the general theory and science behind LC, but I am struggling to figure out how to set up a functioning system using LC as a master inoculator so to speak. Regardless of what you think about LC, can someone give me clarity on how I might attempt to do this? My apologies if there is a TEK for this. I have searched through the forum and spent a lot of time trying to figure out on my own. Even like a sentence explanation saying briefly how I might do this would suffice.
I would certainly use agar as I am very comfortable with it, but I cannot open my spawn bags and they must be injected. If there is another way to perhaps use agar or something other than LC that is less contaminate prone while still using syringes, let me know. I will not be opening the LC jars after sterilization.
Any clarity on this would be much appreciated. And yes, fuck me sideways for wanting to use LC
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mushhead
Potato Devourer



Registered: 08/22/14
Posts: 2,215
Loc: Dimension X-124
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shadyshroomie
MycoCurious



Registered: 10/29/20
Posts: 132
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Fuck yeah. Thank you. Been searching...
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sonoramo
Contaminant



Registered: 02/27/19
Posts: 851
Loc: California, baby!
Last seen: 6 hours, 51 minutes
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If you're going to invest all that money and effort in sterile grain bags, you really want to make sure you're injecting them with something that's not loaded with contaminants. You could prepare agar in small jars with the same injection-friendly lids as grain jars. They'd fit in a small PC, or you could reasonably go with boiling water to sterilize the agar. Several people have even had success injecting sterile water to such a jar to make the LC.
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mushhead
Potato Devourer



Registered: 08/22/14
Posts: 2,215
Loc: Dimension X-124
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Sonoramo take a look at the links I posted haha. funny guy :3
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shadyshroomie
MycoCurious



Registered: 10/29/20
Posts: 132
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You guys are incredible. I really wanted to avoid LC but did not know how to have the same essential process to inoculate and what not. Preciate ya.
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sonoramo
Contaminant



Registered: 02/27/19
Posts: 851
Loc: California, baby!
Last seen: 6 hours, 51 minutes
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Quote:
mushhead said: Sonoramo take a look at the links I posted haha. funny guy :3
He said he wanted to avoid using a big PC. I was just trying to be helpful 
BTW: the little window that pops up when I point to your links is the same for both links. Did you intend to post two links?
Edited by sonoramo (11/25/20 06:30 PM)
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mushhead
Potato Devourer



Registered: 08/22/14
Posts: 2,215
Loc: Dimension X-124
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Yeah I realized I posted it twice but didn't care to remove it. He's gonna have to use a PC or some kind of autoclave to do LC with. He doesn't exactly have a choice, he'll read up and learn proper like.
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shadyshroomie
MycoCurious



Registered: 10/29/20
Posts: 132
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Was going to use instantpot for LC but for obvious reasons have parted ways with any plan to touch LC 
Curious of application of LC that makes sense Perhaps mass gallon jugs for large fields? idk I am new to mycology
Loving learning living ya dig
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