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OfflinekunzyMAGI24
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B+ and Albino A+ Cross Grow Report
    #27057208 - 11/25/20 02:43 PM (3 years, 2 months ago)

So a friend is doing a cross between two strains of Psilocybe Cubensis.The reason for this cross was not the original intent of this project. The original intent was for a B+ harvest only.

The two plastic containers are filled with Rye flour, fine milled vermiculite, distilled water, and pine shavings.

The first inoculate was of B+ liquid culture. A week went by, and there was no signs of colonization, or contamination.

Upon inspection, it was noted on that from the outside, the substrate looked dry. My friend proceeded to fill a couple of syringes of purified distilled water, that was just boiled and cooled down. He then filled the containers each with two syringe-fulls of water, and then shook the container.

My friend was impatient, and did not want to wait and find out if the liquid culture that was bought on Ebay was a dud. So he used another liquid culture of Albino A+.

Three days later, there is first signs of colonization. A beautiful, healthy patch of mycelium in the corner of the plastic container.

I will periodically keep this thread updated, from inoculation to spore prints and trip reports of this strain that may or may not be a cross between Albino A+ and B+ Psilocybe Cubensis.

Below is a photo of the beginning signs of colonization.



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*I trade spores, i dont care what they are as long as i dont already have them.

*Panaleous Cinctilous Wanted!



Edited by kunzyMAGI24 (11/26/20 04:24 PM)


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OfflineLtLurker
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Re: B+ and Albino A+ Cross [Re: kunzyMAGI24]
    #27057218 - 11/25/20 02:51 PM (3 years, 2 months ago)

It's gonna grow mushrooms and you're not gonna be sure what the genetics are. Leucistics(AA+) can come out normal looking, and Normal varieties(B+) can produce some leucistics. Since leucistic is a recessive trait, you might also make a generation of carriers that looks normal, but the offspring have a decent chance at leucistic.


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Invisiblemushhead
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Re: B+ and Albino A+ Cross [Re: LtLurker]
    #27057224 - 11/25/20 02:56 PM (3 years, 2 months ago)

Plus this is a dikaryotic cross which in most cases doesn't end up crossing.
You want to cross monokaryotic spore with other monos to create dikaryotic crosses.
Mixing live dikaryotic mycelia isn't the best way to go about it.
in most cases it wont cross and the two cubes grow along side each other.


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OfflinekunzyMAGI24
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Re: B+ and Albino A+ Cross [Re: mushhead]
    #27057228 - 11/25/20 02:58 PM (3 years, 2 months ago)

How would you recommend doing a monocariotic cross? :smile:


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*I trade spores, i dont care what they are as long as i dont already have them.

*Panaleous Cinctilous Wanted!



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OfflineLtLurker
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Re: B+ and Albino A+ Cross [Re: kunzyMAGI24]
    #27057232 - 11/25/20 03:01 PM (3 years, 2 months ago)

Quote:

kunzyMAGI24 said:
How would you recommend doing a monocariotic cross? :smile:



This is an awesome question. It's harder. You need to dilute the shit out of a syringe and hope some plate germ is mono under a microscope for each variety.

It's not practical to everyone. Dikaryotic crosses are do-able. It's been done a couple times.


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OfflinekunzyMAGI24
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Re: B+ and Albino A+ Cross [Re: LtLurker]
    #27057236 - 11/25/20 03:02 PM (3 years, 2 months ago)

Good to know. Also, is it possible to cross two species of Psilocybe? Such as tampenesis and baeocystis?


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*I trade spores, i dont care what they are as long as i dont already have them.

*Panaleous Cinctilous Wanted!



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OfflineLtLurker
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Re: B+ and Albino A+ Cross [Re: kunzyMAGI24]
    #27057238 - 11/25/20 03:04 PM (3 years, 2 months ago)

Afaik, no they're too different.


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Invisiblemushhead
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Re: B+ and Albino A+ Cross [Re: kunzyMAGI24] * 1
    #27057239 - 11/25/20 03:04 PM (3 years, 2 months ago)

well its easier if you use agar with an inoculation rod and spore prints to spread the two varieties it is that you're crossing for germination.
You then take transfers and grow them out to see if the cross took.
It's a process.
Cubensis is cubensis is cubensis.
Other psilocybe species usually don't cross. Cubes are different because they have a huge diversity.


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OfflinekunzyMAGI24
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Re: B+ and Albino A+ Cross [Re: LtLurker]
    #27057240 - 11/25/20 03:05 PM (3 years, 2 months ago)

Ohh okay. I suppose it would be like crossing a chihuahua and a wolf. Lol


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*I trade spores, i dont care what they are as long as i dont already have them.

*Panaleous Cinctilous Wanted!



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Invisiblemushhead
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Re: B+ and Albino A+ Cross [Re: kunzyMAGI24] * 1
    #27057246 - 11/25/20 03:09 PM (3 years, 2 months ago)



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OfflinekunzyMAGI24
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Re: B+ and Albino A+ Cross [Re: mushhead]
    #27057249 - 11/25/20 03:10 PM (3 years, 2 months ago)

Thank you so much. I greatly appreciate it


--------------------
*I trade spores, i dont care what they are as long as i dont already have them.

*Panaleous Cinctilous Wanted!



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Invisiblemushhead
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Re: B+ and Albino A+ Cross [Re: kunzyMAGI24]
    #27057255 - 11/25/20 03:13 PM (3 years, 2 months ago)

not a problem man, happy crossing!
I hope this gets you into agar work and the like.


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OfflinekunzyMAGI24
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Re: B+ and Albino A+ Cross [Re: mushhead]
    #27057297 - 11/25/20 03:39 PM (3 years, 2 months ago)

Im intimidated by agar, but im getting a eppendorf tube of Gymnopilus luteofolius from etsy, and i want to get my money's worth. I was thinking about just throwing it in a woodchip and vermiculite jar and see if i can get prints that way. Gymnopolis is my favorite. Most visual experience I've ever had.


--------------------
*I trade spores, i dont care what they are as long as i dont already have them.

*Panaleous Cinctilous Wanted!



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Invisiblemushhead
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Re: B+ and Albino A+ Cross [Re: kunzyMAGI24]
    #27057303 - 11/25/20 03:40 PM (3 years, 2 months ago)

Don't be intimidated and honestly you'd spend less money working from agar and gathering your own spores.
It just takes knowledge and practice.
Buying LC online is like buying pre-grown plants from some store.
Sure you get fruit and grew the plant a little but what did you learn?
You learned how to fruit a mushroom. Thats it.
Starting from agar you get to learn how it grows from spore to fruit which in the long run better for your future endeavors in cultivation.


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Re: B+ and Albino A+ Cross [Re: mushhead] * 2
    #27057310 - 11/25/20 03:43 PM (3 years, 2 months ago)

A few things:

Firstly, you can cross a chihuahua and a wolf.

Secondly, and similarly, hybrids within the same genus is possible, and doesn't always produce infertile offspring. Beefalo (cattle x bison) is an example. I don't think there are many bison left that do not have any cattle genes in them.

As far as I know, there have been no attempts to create a hybrid psilocybes of different species. This doesn't mean it can't happen though.

Thirdly, dikaryotic crosses can certainly happen. It just only happens about 5% of the time, but each mating pair of spores is a dikaryon, so if you are attempting to cross two MS cultures (not clones), then there are dozens of different Dikaryons (strains), some of which WILL be compatible and hybridize with eachother. Its like rolling a 20 sided dice dozens of times. You are bound to roll a 1 at least once. Take a look at the Strain crossing thread for more info


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OfflinekunzyMAGI24
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Re: B+ and Albino A+ Cross [Re: One of Us]
    #27057314 - 11/25/20 03:45 PM (3 years, 2 months ago)

Thank you so much man! Make sure to stay tuned for the continuation of this grow report!


--------------------
*I trade spores, i dont care what they are as long as i dont already have them.

*Panaleous Cinctilous Wanted!



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Invisiblemushhead
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Re: B+ and Albino A+ Cross [Re: One of Us]
    #27057316 - 11/25/20 03:45 PM (3 years, 2 months ago)

I was just looking for that thread to share here.


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OfflinekunzyMAGI24
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Re: B+ and Albino A+ Cross [Re: mushhead]
    #27058203 - 11/26/20 07:17 AM (3 years, 2 months ago)

Good news, a huge rush of colonization has taken place over night with one of the plastic containers. I had to enhance the lighting in these pictures, so I'm aware it looks a little off. I can provide the unedited pics if anyone is curious. But otherwise, i think these make it easier to see the mycelium.



--------------------
*I trade spores, i dont care what they are as long as i dont already have them.

*Panaleous Cinctilous Wanted!



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InvisibleMycolorado
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Re: B+ and Albino A+ Cross [Re: kunzyMAGI24]
    #27058339 - 11/26/20 09:09 AM (3 years, 2 months ago)

I’d steer clear of adding pine to your cube projects as they don’t grow on wood.  Pine is also a poor choice for wood lovers as it contains a lot of compounds that inhibit fungal growth.


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OfflinekunzyMAGI24
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Re: B+ and Albino A+ Cross [Re: Mycolorado]
    #27058373 - 11/26/20 09:32 AM (3 years, 2 months ago)

Good to know. Was under the impression that pine would be great because he grew panaleous cinctilous with pine and had great success.

I'll make sure to let him know NOT to use pine. Thank you


--------------------
*I trade spores, i dont care what they are as long as i dont already have them.

*Panaleous Cinctilous Wanted!



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