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OfflineelJefeLoco
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Registered: 01/11/20
Posts: 21
Last seen: 1 month, 24 days
Inoculating grain with Liquid Culture. Many fails. Any tips?
    #27053526 - 11/23/20 09:59 AM (1 month, 27 days ago)

Have tried different strains, different grains, different amount of inoculant but can't get consistent (or any) results.

I've had lots of success going from agar->grains but want to bypass the agar if I can as I get lots of contams that way.

Any tips?


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Invisiblebigfootscreepyuncl
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Registered: 11/15/20
Posts: 267
Loc: Gamehenge
Re: Inoculating grain with Liquid Culture. Many fails. Any tips? [Re: elJefeLoco]
    #27055480 - 11/24/20 02:41 PM (1 month, 26 days ago)

Are you using MS syringes? How were you inoculating the agar? I would keep doing agar to grain since that's how you're having success and it seems like that's how all the TCs on here operate.

Take my advice with a grain of salt since I'm a complete noob. In fact, I'm still preparing for my first project. My plan is to use a MS syringe to colonize 24 BRF cakes, use the ones that don't get contaminated to spawn multiple shoeboxes and start cloning to agar so I don't have to deal with syringes and I'll hopefully have better odds at avoiding contaminations in the future.

Not much for advice. A shameless thread bump if nothing else. Cheers, mate!


--------------------
Bigfoots LAGM21


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OfflineelJefeLoco
L'etranger

Registered: 01/11/20
Posts: 21
Last seen: 1 month, 24 days
Re: Inoculating grain with Liquid Culture. Many fails. Any tips? [Re: bigfootscreepyuncl]
    #27055640 - 11/24/20 04:44 PM (1 month, 26 days ago)

I've tried both MS and liquid culture.  Same result.


Sounds like you have  a plan.  Good luck.


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OfflineCocaineBuffet
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Registered: 08/30/19
Posts: 980
Last seen: 13 hours, 34 minutes
Re: Inoculating grain with Liquid Culture. Many fails. Any tips? [Re: elJefeLoco] * 1
    #27056148 - 11/24/20 10:00 PM (1 month, 26 days ago)

What did you inoculate the LC with? If you are still trying to inoculate grains with spores its likely

A. You used a dirty syringe full of spores to LC and then your LC is contaminated

B. The agar you put in the LC was not clean enough and the LC is contaminated


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