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Offlinekarri0n
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Updated: Chicken Of The Woods, Lion's Mane, Turkey Tail, Maitake * 2
    #26990509 - 10/17/20 06:55 PM (3 years, 3 months ago)

I'm going to be starting up a few gourmet and medicinal varieties.



I've got some Chicken of the woods, Laetiporus Sulphurerus plug spawn, some Lion's Mane plug spawn, and some Hen of The woods/Maitake, Grifola Frondosa grain spawn.

I've got a polypore with the same coloration and banding as a Turkey Tail, Trametes Versicolor, but the pores are too large. It may be another variety of trametes but I haven't been able to find anything that looks quite right other than Turkey Tail for the coloration. It may be another genus altogether. I put this on cardboard and have some mycelium which grew through it that I will be taking a very tiny sample from, to put to agar.

I've got an 8" Dia. Maple log That I've cut into two 11 inch lengths. A tree service was taking it down and I saw the perfect opportunity to grab a nice log. I took the chainsaw chips from this because I loved how fluffy they were compared to any other type of sawdust. I think the mycelium will have a field day with it.

It has been sitting indoors for 1 week since cutting and I kept it in the oven at 225 for about 3-4 hours until I saw some checking on the ends. It seems maple is the one wood that mushrooms prefer to have much more seasoned(which really just means dry, causing the grain to pull apart. once the grain pulls apart, there is room for the mycelium to crawl through. It then needs moisture. This is why we re-hydrate after drying.) than others, and I also wanted to ward off any contams from the outside of the bark.

I put the logs into a monotub and stuck them out in the rain yesterday. These will get drilled, then inoculated with Lion's Mane plug spawn and Chicken of the Woods plug spawn.


Chainsaw dust before hydration or PC



I did the following:

Sawdust Jars:
- Put the chainsaw dust 2/3 the way to the top
- turned on the sink to the very lowest setting with a solid stream
- passed the jar under it once, quickly
- closed the jar and shook
- added about a teaspoon of BRF
- shook and felt it. It's not wet but its very slightly moist.

Dowels for plug spawn:
- pre-purchased birch mushroom spawn dowels with spiral groove
- put the dowels into half pint jars
- added a pinch of chainsaw dust to add some inoculation points
- filled the jars to the top with tap water and closed the lid to submerge the dowels for 24 hr
- dumped them into a metal strainer and let them air dry until the surface was dry
- put them back in the jars and shook.

Left to right: Birch dowels with sawdust, Sawdust with a dash of water and about 1 tsp BRF, Oats boiled for 20 minutes and cooled in metal strainers for 4 hours. These are all going into the PC  at 15PSI + 1 quarter on top for 90 minutes.



Next Steps will be:

- Drill Logs, Hammer in Lion's Mane Plugs and Chicken Plugs, wax the ends
- Put Maitake Grifola Frondosa grain spawn to dowels
- Put Chicken of the WoodsLaetiporus Sulphureus dowels to Sawdust Spawn
- Put Lion's Mane Hericium erinaceus dowels to sawdust spawn
- Transfer unknown Polypore mycelium to agar


Edited by karri0n (01/29/21 09:36 PM)


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Offlinekarri0n
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Re: Chicken Of The Woods, Hen of The Woods/Maitake, Lion's Mane, Turkey Tail lookalike [Re: karri0n]
    #27016421 - 11/01/20 09:05 PM (3 years, 3 months ago)

Update:

The Trametes wild specimen was too contaminated, and molded over on agar.

Here is Chicken of the woods pinning on a birch dowel!:
Edit: that's not what it is. Maybe some bark from processing the logs got in there. Got excited.



Lion's mane dowel colonizing agar. It has an interesting pattern of "spots" all over:


Here is agar that I streaked with a Lion's Mane dowel. Two blurry pics, one closer than the other. You can see the same "spots" pattern.


Chicken of the woods colonizing agar from a dowel. It is very fuzzy. this seemed to do nothing for a while then suddenly colonized very quickly. 3 days of nothing, then this growth in another 3. I know it's not mold because it has the distinctive orange color where it gets thicker on the dowel.

This species is known to have very light, fluffy mycelium. The pics aren't great for seeing the details of the mycelium, but you can see just how far it colonized, looking through the bottom of the plate.


Edited by karri0n (11/02/20 08:03 AM)


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Offlinedeadmandave
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Re: Chicken Of The Woods, Hen of The Woods/Maitake, Lion's Mane, Turkey Tail lookalike [Re: karri0n]
    #27017175 - 11/02/20 09:53 AM (3 years, 3 months ago)

I havent seen wood prepared by first drying it then re-hydrating. Is this something you have tried before?

I like the species you are working with. Good luck.


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OfflineShu
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Re: Chicken Of The Woods, Hen of The Woods/Maitake, Lion's Mane, Turkey Tail lookalike [Re: karri0n]
    #27017201 - 11/02/20 10:06 AM (3 years, 3 months ago)

:popcorn:


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Offlinekarri0n
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Re: Chicken Of The Woods, Hen of The Woods/Maitake, Lion's Mane, Turkey Tail lookalike [Re: deadmandave]
    #27017402 - 11/02/20 12:27 PM (3 years, 3 months ago)

Quote:

deadmandave said:
I havent seen wood prepared by first drying it then re-hydrating. Is this something you have tried before?

I like the species you are working with. Good luck.




Not something I've done, but I've seen recommendations to let the logs sit 2 weeks then soak 24 hours, and in a separate publication a number of results that showed all the results working best on hardwood seasoned for only a couple weeks, except for maple which performed best after much longer.

I wouldn't consider the wood dried - kiln drying even split logs takes much longer and I do it at higher temperatures. This just caused slight checking on the ends and hopefully killed any non-target bacteria or fungi in the outer bit of the wood.

If these logs don't work out, that's why I'm getting everything onto agar, grain, and sawdust as well.


--------------------

Panaeolus Bisporus


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Offlinekarri0n
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Re: Chicken Of The Woods, Hen of The Woods/Maitake, Lion's Mane, Turkey Tail lookalike [Re: karri0n]
    #27052990 - 11/22/20 09:14 PM (3 years, 2 months ago)

Some Updates:

Here is the Chicken of the Woods, Laetiporus Sulphureus, sawdust spawn inoculated 10/25:


***

I had really counted out this Lion's Mane, Hericium Erinaceus, sawdust spawn, also inoculated 10/25/20, because it was way too moist. It managed to come to party anyway, and it seems to have actually squeezed the moisture upward from the bottom of the jar and and consolidated significantly in that area.


***

I inoculated oats using the Chicken sawdust spawn seen above on 11/11/20, Shook once, here is the recovery much stronger probably 7 days after the shake:

***

Because I thought the Lion's Mane sawdust didn't make it, I instead used agar to inoculate oats 11/11/20, here it is 11 days later:

***

And, last but not least, I thiiiiiiink the Hen of the Woods/Maitake,  Grifola Frondosa, is doing an ok job of colonizing the birch dowel plugs at this point:


--------------------

Panaeolus Bisporus


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Offlinekarri0n
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Re: Chicken Of The Woods, Hen of The Woods/Maitake, Lion's Mane, Turkey Tail lookalike [Re: karri0n]
    #27053191 - 11/22/20 10:31 PM (3 years, 2 months ago)

I found some wild Turkey Tail



and some Reishi, likely Ganoderma Tsugae as opposed to Lucidum, though I don't know if it was a hemlock tree that these were growing from. I am going to have to get better at tree ID if I am going to improve in fungus ID.



I am attempting to collect spores or clone these. The Reishi seems like it might be long dead, so I may be SOL on that front, but this area was completely overrun with Reishi. I can go back in the spring if necessary.


--------------------

Panaeolus Bisporus


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Offlinedeadmandave
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Re: Chicken Of The Woods, Hen of The Woods/Maitake, Lion's Mane, Turkey Tail lookalike [Re: karri0n]
    #27053192 - 11/22/20 10:35 PM (3 years, 2 months ago)

Your jars are lookin good. that lions mane on the wet substrate is surprising and awesome.


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Re: Chicken Of The Woods, Hen of The Woods/Maitake, Lion's Mane, Turkey Tail lookalike [Re: deadmandave]
    #27053301 - 11/23/20 02:36 AM (3 years, 2 months ago)

You should put the reishi on agar. It will come back to life.


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Re: Chicken Of The Woods, Hen of The Woods/Maitake, Lion's Mane, Turkey Tail lookalike [Re: nosf3r4tu]
    #27053432 - 11/23/20 06:19 AM (3 years, 2 months ago)

Ok so Hen of the Woods is my absolute fav gourmet. I enjoy trotting through the woods and picking it every year. I currently grow oysters, they are alot of fun. How much harder is it to get Hen of the Woods going? I know they are extremely slow growers in the wild, is it worth getting into?


--------------------
Be patient and keep it simple.


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Offlinekarri0n
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Re: Chicken Of The Woods, Hen of The Woods/Maitake, Lion's Mane, Turkey Tail lookalike [Re: sendmehummus] * 1
    #27054126 - 11/23/20 03:05 PM (3 years, 2 months ago)

Quote:

sendmehummus said:
Ok so Hen of the Woods is my absolute fav gourmet. I enjoy trotting through the woods and picking it every year. I currently grow oysters, they are alot of fun. How much harder is it to get Hen of the Woods going? I know they are extremely slow growers in the wild, is it worth getting into?




It's supposedly one of the harder ones to get to fruit indoors and needs cold temps to do so. I don't know that I will have any success with it, but I know they do bottle culture commercially in Japan of Maitake.

Logs however, if you inoculate them properly and keep them in an appropriately shaded/moist spot outdoors, should fruit pretty well as far as I know.


--------------------

Panaeolus Bisporus


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OfflineForresterM
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Re: Chicken Of The Woods, Hen of The Woods/Maitake, Lion's Mane, Turkey Tail lookalike [Re: karri0n]
    #27054316 - 11/23/20 05:27 PM (3 years, 2 months ago)

That reishi does look like the tsugae around here usually looks.  I wouldn't bother to clone it if it's tsugae unless you want a challenge, it likely won't grow indoors.


--------------------
Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here.
-------------------

Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.


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InvisibleIdiotCircusBoy
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Re: Chicken Of The Woods, Hen of The Woods/Maitake, Lion's Mane, Turkey Tail lookalike [Re: karri0n]
    #27054539 - 11/23/20 07:51 PM (3 years, 2 months ago)

Did you use charcoal in the agar for the Lions Mane?  Got any pics?


--------------------
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Offlinekarri0n
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Re: Chicken Of The Woods, Hen of The Woods/Maitake, Lion's Mane, Turkey Tail lookalike [Re: Forrester]
    #27054756 - 11/23/20 11:08 PM (3 years, 2 months ago)

Quote:

Forrester said:
That reishi does look like the tsugae around here usually looks.  I wouldn't bother to clone it if it's tsugae unless you want a challenge, it likely won't grow indoors.




Dang, good to know, I appreciate that. If I can get it to do anything in agar I will probably put it into some birch plugs and try for some hemlock logs and stick em outside.


--------------------

Panaeolus Bisporus


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Offlinekarri0n
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Re: Chicken Of The Woods, Hen of The Woods/Maitake, Lion's Mane, Turkey Tail lookalike [Re: IdiotCircusBoy]
    #27055373 - 11/24/20 11:31 AM (3 years, 2 months ago)

Quote:

IdiotCircusBoy said:
Did you use charcoal in the agar for the Lions Mane?  Got any pics?




No charcoal. I used the leftover water after boiling oats, agar-agar at 10g per 500ml, and some blue food coloring. Is charcoal beneficial in the agar for wood loving species?

I swiped a colonized lion's mane dowel over this plate and let it colonize.


Then I put the dowel inside this plate.I don't have colonization pics but this one colonized more fully and faster, not surprisingly. I took the transfer from this plate that I used to inoculate oats and an LC.


I did the same process with the Laetiporus. Here is the dowel swipe plate. You can see where I took a transfer to make an LC:


Here is the chicken of the woods dowel plate. It's harder to see due to the condensation and the myc getting onto the sides. This one went completely nuts in this plate.


--------------------

Panaeolus Bisporus


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Re: Chicken Of The Woods, Hen of The Woods/Maitake, Lion's Mane, Turkey Tail lookalike [Re: karri0n]
    #27055411 - 11/24/20 11:54 AM (3 years, 2 months ago)

Quote:

karri0n said:
Is charcoal beneficial in the agar for wood loving species?





I saw your Lion's Mane sawdust pics and thought the Agar wedges were black(I thought cause of charcoal).  I now see they are green.  I'm dumb because I forgot the glasses I wear for computer block blue light.  :facepalm3:


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Offlinekarri0n
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Re: Chicken Of The Woods, Hen of The Woods/Maitake, Lion's Mane, Turkey Tail lookalike [Re: nosf3r4tu]
    #27063682 - 11/29/20 08:23 PM (3 years, 2 months ago)

Quote:

nosf3r4tu said:
You should put the reishi on agar. It will come back to life.




Well, I did, and it did. I'm amazed at the speed it's colonizing the plate. Here it is after 4 days.


I did the same with the Turkey Tail. It's impressive as well.


--------------------

Panaeolus Bisporus


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Offlinekarri0n
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Re: Chicken Of The Woods, Hen of The Woods/Maitake, Lion's Mane, Turkey Tail lookalike [Re: karri0n]
    #27065603 - 11/30/20 10:54 PM (3 years, 2 months ago)

The Reishi plate was completely covered by mycelium 1 day later. I don't think all of it was reishi Mycelium, but most of it was. I took a transfer from the cleanest part to another plate and will see how that looks tomorrow.


I am focusing on the Chicken of the Woods right now because this is a notoriously difficult species to fruit. There are a couple threads on Shroomery with people getting it to fruit, one n Reddit, and this study:

https://link.springer.com/article/10.1007/s11274-012-1230-z


All successful fruitings were done through a 0.5 µm filter, no cut in the bag.

The guy on shroomery who got it to fruit followed the recipe in the study, and I very loosely did as well.

My procedure:

I wanted to try three different recipes for the Laetiporus fruiting block.

I started with a vendor-bought 5lb supplemented sawdust block. I won't be doing that again.

  Supposedly 80% Hardwood sawdust and 20% wheat bran, but when I was mixing it up I found a bunch of composted corn, a bunch of NOT composted corn, and a whole bunch of partially composted things that I'd really rather not know what they were, and wish I didn't have theories :scat: . The whole thing smelled like raisin bran, so it definitely seemed to *mostly* be what it was supposed to be.

According to the research study above, they got poor results with the 10, 20, and 30% supplementation, and started to get good results at the 40 and 50% mark. I decided to go with the following:

"Light Oat Supplemented blend"
1lb Wood/Bran
1/3 Cup Oats with hulls, floured

"Heavy Oat Supplemented blend"
1lb wood/ Bran
2/3 Cup oats with hulls, floured

"Hydrated Whole Oat Supplemented Blend"
1.5lb Wood/Bran
2/3 cup oats with hulls, floured
1/3 cup(Dry) whole oats, hydrated with standard oat grain prep tek


As the best laid plans go, this did not work out.

I have a small PC and won't be opening my 23q Presto until Christmas/Yule, so I am relegated to small and medium bags. This is also the first time I am trying them out. Almost all of the ones the first time through melted.

Gas stove + small PC = Hot Plastic Melty Sides of PC

I ended up mixing the two Heavy and Light supplemented bags into one 2lb block, and the Hydrated oat supplemented blend into another 2lb block.

On the second(read:third) run through, the Hydrated Oat Supplemented bag was a success. The flour supplemented bag melted again. Lesson learned, I can only use the medium size bags, one at a time, as they can stay in the center of the PC.

I have now inoculated the bag supplemented with whole hydrated oats + oat flour + Hulls with roughly half a myco-pint of Chicken of the Woods Grain spawn. I then shifted the way the bag sits so that the colonizing substrate will be able to fruit through the filter patch.


I also inoculated a 2lb block of the supplemented vendor sawdust with 1/2 myco-pint of Lion's Mane Grain spawn.


I've got to PC the oat flour supplemented block again and will inoculate it with the remaining Laetiporus grain spawn.

Unless I get a flow hood, I don't think I want to mess around with G2G with bags any more. It seems like slapping some silicone on the bag to make a SHIP and using LC is going to be a much simpler process for these bags.


--------------------

Panaeolus Bisporus


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OfflineShu
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Re: Chicken Of The Woods, Hen of The Woods/Maitake, Lion's Mane, Turkey Tail lookalike [Re: karri0n]
    #27066645 - 12/01/20 04:02 PM (3 years, 2 months ago)

That's an interesting article but there are 18 ingredients in their substrte mix!


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Offlinekarri0n
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Re: Chicken Of The Woods, Hen of The Woods/Maitake, Lion's Mane, Turkey Tail lookalike [Re: Shu]
    #27066957 - 12/01/20 06:51 PM (3 years, 2 months ago)

Quote:

Shu said:
That's an interesting article but there are 18 ingredients in their substrte mix!




Yes but it's a bit misleading.

Mixed hardwood, Gypsum, dolomite, sugar, salt, then three types of bran and three types of grain. I meet most of this with this grain and substrate mix since we don't remove the Bran from whole grains, minus the dolomite and added sugar. This substrate I got from the vendor has the added corn too heh. We see time and time again that the type of grain doesn't matter, so I don't think that will be the case with this species.

My theory tends to be what makes the biggest difference is that the substrate is supplemented at 50% grain and bran, vs the 20% used by most varieties, as opposed to a few grams of dolomite and chalk being the silver bullet. No one has replicated the study so we can't be sure but I'd bet different grains or even single grains could be done.

That coupled with the injection of the cold water and allowing it to fruit through the filter, never exposing the substrate to open air, seem to be really important to fruiting chickens.


--------------------

Panaeolus Bisporus


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