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OfflineBadgerBB
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I have large pin sets but their growth has been stalled for a long time, please help!
    #27052493 - 11/22/20 04:13 PM (3 years, 2 months ago)

So about 3 weeks ago I got my first pins, over the course of that week I saw more and more pins crop up and they were growing a bit. At the time I wasn't misting and my temperature was the same temperature as my colonisation temperature (my room temperature (66-71F)). I wasn't fanning either as the tek I am using (Uncle Bens Tek) said it wasn't necessary. I was cracking the lids of the monotubs to give FAE and also had a 12/12 light cycle using LED lights. In the following week I started misting twice a day but only the small pins grew to the size of the larger ones (the large pins didn't grow at all). 6 days ago I floated (like a dunk but the substrate floats on top instead of being submerged) all my tubs because I thought the substrate might be too dry but I didn't see any change. 5 days ago (and onwards) I started fanning after misting to see if that would help (it didn't). 4 days ago I managed to sort out a way of heating my tubs so now temperature ranges from 73-75F. 3 days ago I gave the tubs another float to see if that would help. 2 days ago I started to increase the amount of water I do per misting (cause I realised my mister was pretty small) to see if that would make a difference (it didn't). I also started misting the lids and walls as well as the surface but that didn't make a difference either. I also decreased FAE a little bit as I realised I was giving them a bit too much FAE.

I am super stuck at this point and I am not sure what I need to do to get this pins to grow, they have been stalled for about a week and a half now.

My substrate is coco coir and my mushroom variety is Ban Sa Phang Kha if that helps at all.

Here are my pinsets:








Here is the amount of FAE I give the pins:


Here is my setup:


I use a fan (on cool) to circulate air to try and decreases CO2:


I use a space heater for heating:


Pic of lid after misting:


Pic of surfaces after misting:




Size of my mister:


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OnlineA.k.aM
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Re: I have large pin sets but their growth has been stalled for a long time, please help! [Re: BadgerBB]
    #27052659 - 11/22/20 05:37 PM (3 years, 2 months ago)

Sometimes weird stuff happens especially with giant pinsets like that.


When you mist just keep in mind that the purpose is to get little beads of water on the surface that will evaporate, not to water the mushrooms.


--------------------
LAGM2020


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OfflineBadgerBB
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Re: I have large pin sets but their growth has been stalled for a long time, please help! [Re: A.k.a]
    #27052701 - 11/22/20 06:03 PM (3 years, 2 months ago)

Right yea, that is what I aim for when I mist. So I should just keep doing what I am doing or do you have any suggestions on changes I could make?


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InvisibleMateja
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Re: I have large pin sets but their growth has been stalled for a long time, please help! [Re: BadgerBB]
    #27052766 - 11/22/20 06:35 PM (3 years, 2 months ago)

Sounds like everything was going smooth until you started misting. Also pinsets like that sometimes stall but for every pin in every tub to stop growing completely for 10 days obviously seems to be due to some great stress factor, is that a clone by chance in all those tubs?


--------------------
Cakes inside Water Tub


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OfflineBadgerBB
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Re: I have large pin sets but their growth has been stalled for a long time, please help! [Re: Mateja]
    #27052771 - 11/22/20 06:38 PM (3 years, 2 months ago)

I am not sure if they are clones, I sourced the prints off a reliable vendor on reddit. I guess that is true about the misting but I think that is a coincidence since my tubs will need misting correct?


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OfflineBadgerBB
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Re: I have large pin sets but their growth has been stalled for a long time, please help! [Re: Mateja]
    #27052881 - 11/22/20 07:59 PM (3 years, 2 months ago)

However, the spore print I used was a year and a half old, though I did leave the spores in water in a syringe for 5 days to allow them to rehydrate before inoculating


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OfflinePBJ710
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Re: I have large pin sets but their growth has been stalled for a long time, please help! [Re: BadgerBB]
    #27053038 - 11/22/20 09:38 PM (3 years, 2 months ago)

Not sure that this is 'the' answer, but maybe it will spur some ideas.  I see a couple things I would look further into:

That substrate looks really wet - oversaturated substrates can dwarf fruits.  Overmisting can do the same.  Make sure you're using field capacity coir where you can barely squeeze any water out of a handful of it and only misting when there is no droplets on the surface or it turns a lighter shade of brown.

I know you are showing the lid gap as your FAE point, but what is the room's air exchange like?  Maybe a CO2 excess could be stalling them - is there a pilot light or something else be contributing to the CO2 ppm?

Anywho, good luck...I'd love to see that pinset finish fruiting!


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OfflineBLINKfan420
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Re: I have large pin sets but their growth has been stalled for a long time, please help! [Re: BadgerBB]
    #27053058 - 11/22/20 09:45 PM (3 years, 2 months ago)

The age of the spores isn't going to matter at this point.  They obviously germinated and colonized aggressively enough to reach 100% with no obvious contams.  And a pin set like that generally means you have a good batch of genetics.  So all in all you have good spores. (Which are not clones. Maybe the print was taken from a mushroom that was a clone but multispore is multispore.  Clones are made by taking tissue samples from living mushrooms.)

Conditions must have been pretty good to get that pinset also. Now suddenly the conditions are off.  I'm not sure if I see fuzzy feet on those shrooms or not.  But if you have fluffy white mycelium creeping up the stipes then you need more fresh air.

I see the person above me seems to be onto the same idea.^

BLiNK


--------------------



All my pictures are drawn from imagination.  I don't even know what a mushroom is.


Edited by BLINKfan420 (11/22/20 09:45 PM)


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OfflineTomato_tuhmahto
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Re: I have large pin sets but their growth has been stalled for a long time, please help! [Re: PBJ710]
    #27053062 - 11/22/20 09:45 PM (3 years, 2 months ago)

I vote oversaturated. I did the same thing before. I would stop misting, barely leave the lid cracked and leave it alone to see what happens while you start on another tub.


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OfflineBadgerBB
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Re: I have large pin sets but their growth has been stalled for a long time, please help! [Re: BLINKfan420]
    #27053085 - 11/22/20 09:52 PM (3 years, 2 months ago)

Yes they some of the pins do have fuzzy feet. I will try increase FAE more. I will try hold off misting to see if that helps. They might look so wet cause I did float them twice this week. In terms of air exchange, I always keep the door to my room open and my closet door open but windows are pretty much never open in my apartment. Would opening the windows in my room help?


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OfflineBadgerBB
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Re: I have large pin sets but their growth has been stalled for a long time, please help! [Re: Tomato_tuhmahto]
    #27053094 - 11/22/20 09:56 PM (3 years, 2 months ago)

I actually have two tubs that went into fruiting weeks after the other 7 tubs so I am monitoring them closely to see if there is any difference between those 2 and the other 7. Also windows are never open in my apartment so air gets really stale. Should I open up some windows? Could that help?


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OfflineBadgerBB
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Re: I have large pin sets but their growth has been stalled for a long time, please help! [Re: PBJ710]
    #27053128 - 11/22/20 10:00 PM (3 years, 2 months ago)

I definitely used field capacity coir, I actually thought it was too dry a few days ago hence me floating (dunking but substrate floating on top) twice, once 5 days ago and another time 3 days ago. Maybe that is why they look so wet. I'll ease up on the misting and only misting when required rather than blindly sticking to twice a day, maybe that'll help. Also I don't have a pilot light or anything that could be increasing CO2 but I just realised in my apartment windows are like never open and in my room I never open the windows either so could it be the case of stale air? Should I open my windows and see if that makes a difference?


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OfflinePBJ710
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Re: I have large pin sets but their growth has been stalled for a long time, please help! [Re: BadgerBB]
    #27053218 - 11/22/20 11:27 PM (3 years, 2 months ago)

Open windows not needed, just wanted to make sure it wasn't a sealed up bathroom with a gas appliance running in it or something similar.  Everything seems like it is starting to point to overhydration.  Keep an eye on them and post some updates when you can.


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OfflineBadgerBB
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Re: I have large pin sets but their growth has been stalled for a long time, please help! [Re: PBJ710]
    #27053228 - 11/22/20 11:51 PM (3 years, 2 months ago)

Ok. This is what my pin sets were like 12 days ago before I did the floats and when I was misting less. Is this what they should look like when they need to be misted (lighter brown substrate)?









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OfflineBadgerBB
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Re: I have large pin sets but their growth has been stalled for a long time, please help! [Re: BadgerBB]
    #27053797 - 11/23/20 11:29 AM (3 years, 2 months ago)

Bump


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OfflinePBJ710
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Re: I have large pin sets but their growth has been stalled for a long time, please help! [Re: BadgerBB]
    #27053819 - 11/23/20 11:52 AM (3 years, 2 months ago)

Surface mycelium is kinda sketchy in some of those pics, but they look like they could use a light mist if there's no water droplets on the surface of the mycelium patches(the droplets show up much better in pics when a flash is used). 

Think about the color of the coir before you added water to the brick - thats what dry coir looks like.  If you start seeing dusty bits like that show up on the surface, it's an indicator that you're losing moisture so fast that it can't be wicked up from the substrate to replenish it.  As a newer grower, If I discover suspected environmental issues like that I try to make the corrections in small steps over a few days so I can get confirmation as I go that it's working as desired.  It also seems to be less stressful on the mycelium.


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InvisibleStudy The CNS
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Re: I have large pin sets but their growth has been stalled for a long time, please help! [Re: PBJ710]
    #27053829 - 11/23/20 12:02 PM (3 years, 2 months ago)

1. Misting the sides of the bin daily is important.
2. So is fanning the air so it doesn't get "stale."
3. A "cold shock" might induce more pinning and even growth.
4. Looks like it needs a new casing layer of sterilized top soil with coco coir.

You might have to start over. If so, this can still be a valuable experience. On another note, this may be just an oddity that wasn't anybody's fault.


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OfflineCamera93
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Re: I have large pin sets but their growth has been stalled for a long time, please help! [Re: Study The CNS] * 2
    #27053854 - 11/23/20 12:16 PM (3 years, 2 months ago)

Quote:

Study The CNS said:
1. Misting the sides of the bin daily is important.
2. So is fanning the air so it doesn't get "stale."
3. A "cold shock" might induce more pinning and even growth.
4. Looks like it needs a new casing layer of sterilized top soil with coco coir.





:justno:

1. Walls don't grow mushrooms/**misting on any kind of schedule is bound to over saturate your surface. Read the substrate and mist as needed to maintain that nice microclimate. Getting the moisture content nailed down from the start is the way to go.**
2. The air is not getting stale, there is 24/7 passive fae from the gaps on the lid
3. Cubensis do not require a cold shock
4. A sterilized casing layer would contam, maybe you meant pasteurized, in which case I don't really see people "re casing" and certainly not overtop of a pinset and with top soil..**in the case of coir, it can be used as a top layer no sterilizing/pasteurizing required*

Sorry I am not chiming in with an answer, but that advice was outdated incorrect from my understanding


Edited by Camera93 (11/23/20 12:22 PM)


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OfflinePBJ710
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Re: I have large pin sets but their growth has been stalled for a long time, please help! [Re: BadgerBB]
    #27053895 - 11/23/20 12:58 PM (3 years, 2 months ago)

You did provide valuable input though...

Quote:

Camera93 said:

**misting on any kind of schedule is bound to over saturate your surface.  Read the substrate and mist as needed to maintain that nice microclimate. Getting the moisture content nailed down from the start is the way to go.**





It's much more difficult to correct something like this after the tubs have colonized - trust the field capacity of the substrate and watch the surface conditions to tell you when to mist.  You should be able to get through the first flush with 1 or 2 mistings at most.  If you have to mist more to maintain it, you likely have too much FAE occurring.


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OfflineBadgerBB
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Re: I have large pin sets but their growth has been stalled for a long time, please help! [Re: PBJ710]
    #27054018 - 11/23/20 02:07 PM (3 years, 2 months ago)

Alright I took some photos of the surface with flash.




I still find it hard to see water droplets though. But I do notice that the mycelium seems shiny/kinda glistening so I am wondering if that is indicating that it is oversaturated.


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