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Offlineoname_wa
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agar2agar transfer?
    #27052204 - 11/22/20 01:09 PM (3 years, 2 months ago)

This my first time attempting with agar. I have recently inoculated a couple plates with spore syringe and have two healthy-looking plates with good growth. I inoculated about 2-2 1/2 weeks ago. A couple questions...

Do I need to transfer to another plate before putting to grain, if so when should I do the transfer?

Also, had a couple plate with bacteria and healthy growth, should I transfer the healthy growth to a new plate (so far its kinda overlapping with contams/bacteria?





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OfflineAbabyphoenix
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Re: agar2agar transfer? [Re: oname_wa]
    #27052753 - 11/22/20 06:24 PM (3 years, 2 months ago)

Hey there! I just got into agar this year so I'm no expert but for germination plates my general advice is to transfer as soon as you see healthy mycelium (Generally about a dime size) away from the germination plate that usually has some contaminates.

If you look at Pic #3 you can see some slime mold or something in the upper right sector. take a small grain sized transfer from a couple healthy sections (check  the bottom to see if the myc has grown over a contam) and move it to a new plate and mark them T.1 For transfer 1 and continue until you have a clean dish w/ healthy genetics.

Hope this helped :thumbup:


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Edited by Ababyphoenix (11/22/20 06:25 PM)


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OfflineAbabyphoenix
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Re: agar2agar transfer? [Re: oname_wa]
    #27052759 - 11/22/20 06:28 PM (3 years, 2 months ago)

Also heres Stros agar guide that really helps when starting to understand what to look for and do when working with agar :smile:


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:sporedrop::aum:Pho's MushiAventures::aum::sporedrop:
:heartpump:My '21 GLOG:heartpump:
:peace: LAGM 2022 :peace:



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OfflineEarthwormJim
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Re: agar2agar transfer? [Re: Ababyphoenix]
    #27052775 - 11/22/20 06:39 PM (3 years, 2 months ago)

Welcome to the coolest medium in mycology 👍 yes you should transfer to another plate. Select a sample of rhizomorphic growth from the plate furthest away from the contams. Up to 2 transfers may b necessary b4 you have a clean plate but it's that simple. Namaste 🕉️🙏


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I see shrooms of blue
In tubs of white
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All through the night
And I think to myself
What a wonderful world:trippinbawelz:


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Offlineoname_wa
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Re: agar2agar transfer? [Re: Ababyphoenix]
    #27052922 - 11/22/20 08:28 PM (3 years, 2 months ago)

thanks for the feedback and the lead. Lots to learn, really excited to be using agar now. Can't believe I wasn't using this method from the beginning! :grin::thumbup:


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