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Bsdgaou
Registered: 02/20/19
Posts: 340
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Re: Reishi find, clone, and grow [Re: TheGrower]
#26966747 - 10/02/20 11:18 PM (3 years, 5 months ago) |
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Quote:
TheGrower said: Ok something is happening, does this look ok? I used 5g LME and 5g agar : 250ml water. About 6 days in.
Thanks
Take another transfer from 9 or 11 o'clock.
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Quadman
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Re: Reishi find, clone, and grow [Re: Bsdgaou]
#26966782 - 10/02/20 11:52 PM (3 years, 5 months ago) |
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:Looks like mycelium, just really variable. To get a nice culture I would take a transfer from about 11 o'clock, you should be in great shape then imo.
--------------------
Edited by Quadman (10/02/20 11:54 PM)
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TheGrower
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Re: Reishi find, clone, and grow [Re: Quadman]
#26986428 - 10/15/20 04:25 AM (3 years, 5 months ago) |
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I took the transfers. One is going ok the other one contaminated on the outer edge. Funny enough the first one I took transfers from fully colonised it looks great. I’ll post pics later. Thanks all.
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TheGrower
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Re: Reishi find, clone, and grow [Re: TheGrower]
#27022856 - 11/05/20 09:13 AM (3 years, 4 months ago) |
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As above. I forgot to take photos of the first two that I cut up and added to ssd blocks. This is the third one I took that I’m going to use to make more transfers and inoculate a log outside. Updates on the blocks as and when 👍🏻 Thanks
Ps it was so hard to open the Petri dish lol like the mycelium had taken a hold of the lid is that normal?
Edited by TheGrower (11/05/20 09:44 AM)
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Forrester
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Re: Reishi find, clone, and grow [Re: TheGrower]
#27026077 - 11/07/20 04:05 AM (3 years, 4 months ago) |
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Yeah that happens quite a bit
-------------------- Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here. ------------------- Have some medicinal mushrooms and want to get the most out of them? Try this double extraction method.
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ttching8475
Spawn
Registered: 03/01/18
Posts: 739
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Re: Reishi find, clone, and grow [Re: Forrester]
#27026172 - 11/07/20 05:57 AM (3 years, 4 months ago) |
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I found a tree near my house growing conks. For a cloning attempt, use the lighter, leading edge growth on the outside of the conk rather than inner tissue?
-------------------- LAGM 2.022
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Forrester
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Re: Reishi find, clone, and grow [Re: ttching8475]
#27027067 - 11/07/20 03:33 PM (3 years, 4 months ago) |
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Quote:
ttching8475 said: For a cloning attempt, use the lighter, leading edge growth on the outside of the conk rather than inner tissue?
No, you want the white growth edge but the inside of it, not the outside of the mushroom that's touched air and all the crap in it.
-------------------- Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here. ------------------- Have some medicinal mushrooms and want to get the most out of them? Try this double extraction method.
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ttching8475
Spawn
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Posts: 739
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Re: Reishi find, clone, and grow [Re: Forrester]
#27028045 - 11/08/20 07:17 AM (3 years, 4 months ago) |
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Thanks. I phrased that poorly. Always clone with inner tissues
-------------------- LAGM 2.022
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TheGrower
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Re: Reishi find, clone, and grow [Re: ttching8475]
#27050584 - 11/21/20 03:00 PM (3 years, 4 months ago) |
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Hello. One of my blocks contaminated the other is still colonising slowly but surely. The last plate I used in a quart jar with some brf and vermiculite that I wanted to use up. It absolutely tore through the brf and verm! Not sure if anyone’s used brf and verm with reishi but it does perform well at least this one has. Now I’m about to do a little g2g into rye jars that I made up last night. And with the rest of this fully colonised cake I will spawn to a bucket of oak wood chips enriched with wheat bran and some gypsum. Smells so beautiful! Here’s the quart pf cake. Thanks
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Forrester
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Re: Reishi find, clone, and grow [Re: TheGrower]
#27052333 - 11/22/20 02:09 PM (3 years, 4 months ago) |
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Careful spawning to a substrate with wheat bran in it, unless it's sterilized and you're doing so in front of a flow hood, which since you said it's a bucket I assume isn't the case.
Reishi really isn't good at fighting off molds and spawning to a supplemented substrate without sterilizing it is asking for mold. I would leave the wheat bran out.
-------------------- Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here. ------------------- Have some medicinal mushrooms and want to get the most out of them? Try this double extraction method.
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TheGrower
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Re: Reishi find, clone, and grow [Re: Forrester]
#27052461 - 11/22/20 03:46 PM (3 years, 4 months ago) |
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Damn forrester your too late. I really appreciate the advice but I already did the spawn and g2g. I pasteurised the sawdust/bran/gypsum doing the bucket tek. So I can just hope it does not contaminate. If it does I will leave the bran out next time. I still have two rye quart jars and three agar plates that are colonising quite nicely so all is not lost.
Cheers mate.
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TheGrower
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Re: Reishi find, clone, and grow [Re: TheGrower]
#27054084 - 11/23/20 02:38 PM (3 years, 4 months ago) |
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Also check this cool colouring on one of my plates. Is it normal? Thanks
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Forrester
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Re: Reishi find, clone, and grow [Re: TheGrower]
#27054299 - 11/23/20 05:20 PM (3 years, 4 months ago) |
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Not sure, might be some bacteria in there but that's not a worry if it's reishi.
-------------------- Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here. ------------------- Have some medicinal mushrooms and want to get the most out of them? Try this double extraction method.
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Mrcloudy
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Re: Reishi find, clone, and grow [Re: TheGrower]
#27057861 - 11/25/20 09:51 PM (3 years, 3 months ago) |
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Quote:
TheGrower said: Also check this cool colouring on one of my plates. Is it normal? Thanks
Perfectly normal, and quite common in certain strains and species.
-------------------- 10 different Ganoderma species from across the USA AMU MrCloudys guide to North American GanodermaUpdated A rough guide to North American Ganoderma species, with an emphasis on the laccate species.
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TheGrower
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Re: Reishi find, clone, and grow [Re: Mrcloudy]
#27084117 - 12/11/20 02:57 PM (3 years, 3 months ago) |
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A little update for you all. The bucket sawdust/bran I inoculated with brf has almost completely colonised contam free 🤞 at least what I can see as the bucket isn't see through. I'm guessing I've been lucky as forester said it contams easier with bran only being pasteurised. I've also got two smaller mono almost fully colonised they are a vermiculite and coco coir mix also a bit of coffee and gypsum so bit of an experiment 😁. I have x3 four pound bags I just added colonised grain to today they have sawdust bran and gypsum. Some questions please.
I have fully colonised plates which I'd like to store long term what's the best method? For fruiting the bucket I'm thinking a big clear bag and putting the bucket into it few holes at the top and mist as needed? For the mono I'm thinking cracking lids and mist as needed?
Any feedback welcome please 🙏
Have a great day.
Edited by TheGrower (12/12/20 02:38 AM)
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TheGrower
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Re: Reishi find, clone, and grow [Re: TheGrower]
#27091775 - 12/16/20 08:47 AM (3 years, 3 months ago) |
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😵🙃😉 Anyone?
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nosf3r4tu
Registered: 03/26/19
Posts: 775
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Re: Reishi find, clone, and grow [Re: TheGrower]
#27092001 - 12/16/20 11:33 AM (3 years, 3 months ago) |
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Quote:
TheGrower said: A little update for you all. The bucket sawdust/bran I inoculated with brf has almost completely colonised contam free 🤞 at least what I can see as the bucket isn't see through. I'm guessing I've been lucky as forester said it contams easier with bran only being pasteurised. I've also got two smaller mono almost fully colonised they are a vermiculite and coco coir mix also a bit of coffee and gypsum so bit of an experiment 😁. I have x3 four pound bags I just added colonised grain to today they have sawdust bran and gypsum. Some questions please.
I have fully colonised plates which I'd like to store long term what's the best method? For fruiting the bucket I'm thinking a big clear bag and putting the bucket into it few holes at the top and mist as needed? For the mono I'm thinking cracking lids and mist as needed?
Any feedback welcome please 🙏
Have a great day.
Your fruiting plans seem reasonable. As for long term storage you could keep you're plates in the fridge. But don't put them upside down. They will dry. Although I don't know if that would be a problem. I've cloned dried, molded fruits with success and if iirc and the info I've read are corect reishi is not affected by senescence. So you can always take a tissue sample from future fruits. Also colonized sawdust should keep in the fridge for about 18 months.
I'm on my first attempt at fruiting G. Lucidum. I have 5 wild clones I've put in 10 jars a few days ago. 60% oak wood chips and sawdust to 40% hydrated wheat (by volume) + plus to much gypsum... The jars look like they are dry but I think it's because of the extra gypsum. I'll add pics of the jars when there's visible growth.
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nosf3r4tu
Registered: 03/26/19
Posts: 775
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Re: Reishi find, clone, and grow [Re: nosf3r4tu]
#27092013 - 12/16/20 11:39 AM (3 years, 3 months ago) |
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For the bucket I would take the lid of and replace it with a bag.
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TheGrower
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Re: Reishi find, clone, and grow [Re: nosf3r4tu]
#27093574 - 12/17/20 06:13 AM (3 years, 3 months ago) |
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Cheers for the reply mate. I'm currently growing Turkey Tail and lions mane as well as my Reishi attempt. The lions mane is bought from a vendor online, the TT and Reishi were wild clones. Good luck on your attempts I will keep this thread updated.
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nosf3r4tu
Registered: 03/26/19
Posts: 775
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Re: Reishi find, clone, and grow [Re: TheGrower]
#27095172 - 12/18/20 12:56 AM (3 years, 3 months ago) |
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And here are my best looking jars.
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