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Wrightii
Stranger Danger



Registered: 05/15/20
Posts: 216
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Can I save this LC?
#27050142 - 11/21/20 09:35 AM (3 years, 2 months ago) |
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I started a LC of an agar clone of lionsmane I found growing in the wild. I unfortunatly forgot to add my magnetic stirrer. As you can see colonization is happening, but shaking the jar doesn't break up the mycelium whatsoever.
 Any advice on using LC without any stirring device? Or am I better off just starting a new LC?
I know lionsmane is not a psychedelic, but I feel mushroom cultivation gets more viewers. Plus, it is still relevant for other types of mushrooms too ofcourse:)
Cheers! -Wii
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diegosf
scientific dreamer



Registered: 06/30/20
Posts: 93
Last seen: 2 months, 10 days
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Re: Can I save this LC? [Re: Wrightii]
#27050444 - 11/21/20 01:08 PM (3 years, 2 months ago) |
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I have a Lc just like yours, but the mycelium is way bigger.. Still wondering what can I do with it, because I forgot to put the stir bar/glass inside and NO WAY it’s going to get through the needle..
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coversall
إِنْ شَاءَ ٱللَهُ



Registered: 06/06/20
Posts: 2,749
Loc: संसार
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Re: Can I save this LC? [Re: diegosf]
#27050463 - 11/21/20 01:28 PM (3 years, 2 months ago) |
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I mean this isn't much to go off because A) it's my first LC and B) it's not been used yet to know that's it's clean. HOWEVER, here are my results without any stirrers.


Plenty of growth, don't know if it's what I want to be growing. But I've been giving it a good swill around twice a day, making efforts not to get it to go to the lid.
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PBJ710
Strangler


Registered: 07/05/19
Posts: 2,623
Last seen: 18 days, 13 hours
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Re: Can I save this LC? [Re: coversall]
#27050505 - 11/21/20 01:58 PM (3 years, 2 months ago) |
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Why not suck up the mycelium into a sterile syringe and then use it to inoculate a fresh LC jar with a stirrer already PC'd in it?
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coversall
إِنْ شَاءَ ٱللَهُ



Registered: 06/06/20
Posts: 2,749
Loc: संसार
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Re: Can I save this LC? [Re: PBJ710]
#27050513 - 11/21/20 02:08 PM (3 years, 2 months ago) |
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That's definitley an option but increases the chances of contam with two exchanges. I'm guessing this would be safer if both jars had SHIPs?
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Wrightii
Stranger Danger



Registered: 05/15/20
Posts: 216
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Re: Can I save this LC? [Re: coversall]
#27050519 - 11/21/20 02:14 PM (3 years, 2 months ago) |
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Problem is that this mycelium is really tough, making it (as seems) practically impossible to break up by just shaking. Guess I'm just going to leave it for a few more days to see if more colonization is going to change the "suck-up-ability", and if not just start a new one.
Thanks for the replies! If I find a way to break up the mycelium, I'll update here:)
Cheers! -Wii
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PBJ710
Strangler


Registered: 07/05/19
Posts: 2,623
Last seen: 18 days, 13 hours
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Re: Can I save this LC? [Re: PBJ710]
#27050525 - 11/21/20 02:19 PM (3 years, 2 months ago) |
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It would obviously be better, but can't change that now if it's not already there without even more risk.
Real world time that the jar is open in a SAB should be pretty minimal if you plan things out and position everything where you will need it ahead of time. Wipe down the destination SHP (if it has one - if not make sure the lid is loose) just before you open the current LC jar and have your destination LC jar waiting to receive the inoculation. Don't even set the syringe down between them.
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