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OfflineDj_karma_magnet
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Registered: 04/03/19
Posts: 203
Last seen: 4 months, 5 days
Help!!! Inoculated 11/11, very little growth
    #27049666 - 11/20/20 10:26 PM (3 years, 2 months ago)

I pc’d 10 bags of rye berries (1.5 lb after hydrating each) and had 4 bags of uncle bens (I know this is very frowned upon, but it was the last of my bags and I’m trying to move away from this tek) and I split them between the bags. Golden teacher spores. 12 cc syringe, so some bags only got 1/2 cc. I hardly see any growth on the rye berries but the uncle bens seem to be doing fine, some of the rye berries have a little growth but not much. My apartment temp gets down to like 65. Any suggestions? Anything I can do to speed up the process or am I fucked? What can I do next time?


Should mention the bags were pressure cooked individually in a small electric pot because I’m too afraid to use a real pressure cooker, and for good reason. I burned myself even on the instant pot.


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Offlineglive
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Registered: 10/11/19
Posts: 72
Last seen: 4 months, 16 days
Re: Help!!! Inoculated 11/11, very little growth [Re: Dj_karma_magnet]
    #27049831 - 11/21/20 03:27 AM (3 years, 2 months ago)

It would help to see some pictures, but for me when using rye/spore syringe it will usually take at least a week to see any sign of growth at all, if not more. It's only been ten days so wait and see how they go. You can shake them at 20-30% colonization and they generally speed up drastically from there.


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Invisiblecoversall
إِنْ شَاءَ ٱللَهُ
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Registered: 06/06/20
Posts: 2,749
Loc: संसार
Re: Help!!! Inoculated 11/11, very little growth [Re: glive]
    #27049834 - 11/21/20 03:33 AM (3 years, 2 months ago)

How'd you prep the rye?

Spores to grain is risky, there is a chance the syringes weren't clean. This is why agar is considered a wise move before going to grain. Time will tell though.


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..:: E V E R Y  ::..

..:: New? Start here. ::..
..:: How I Panaeolus. From Agar to Tea ::..


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OfflineDj_karma_magnet
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Registered: 04/03/19
Posts: 203
Last seen: 4 months, 5 days
Re: Help!!! Inoculated 11/11, very little growth [Re: coversall]
    #27050193 - 11/21/20 10:03 AM (3 years, 2 months ago)

I soaked the rye for at least 12 hours than boiled it for about 15 mins, until some grains were popped. I flame sterilized the needle before every injection


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Invisiblecoversall
إِنْ شَاءَ ٱللَهُ
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Registered: 06/06/20
Posts: 2,749
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Re: Help!!! Inoculated 11/11, very little growth [Re: Dj_karma_magnet]
    #27050285 - 11/21/20 10:46 AM (3 years, 2 months ago)

Wait it out but the syringes could have been less than clean.


--------------------

..:: E V E R Y  ::..

..:: New? Start here. ::..
..:: How I Panaeolus. From Agar to Tea ::..


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