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cybernet said:
Gurus,

I resurrected some spore prints(B+) from 13 years ago.  They where printed on index cards, dried, sealed with tape and sealed in zip lock.  Performed, with sterile technique in SAB, inoculation to potato dextrose agar with flamed inoculation loop. Got good amount of spores as the black spots visible on agar.  Put in incubator at 80 deg F and about 75% humidity.
After 2 weeks, no signs of growth or contamination.

Started another set of agar plates with oat soak water with more B+ and GT prints 1 week ago.  Same technique and same incubation.  Also, no signs of growth or contamination.

Should I just wait?  Should I try and soak some spores in sterile water for 24 hours and try more plates?

Should I spring for a microscope and see if I can see if the spores are still viable?  I thought the spores are pretty dam tough.

Any suggestions appreciated.

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A microscope (I have two) won't help you distinguish dead spores from viable ones. It isn't like they'll move around or something. Agar is the best way to make that differentiation, and you seem to already have your answer.

Last year a friend gave me a B+ print on foil. It looked great, but after 3 rounds of trying on different agar recipes, I could never get any growth out of it at all. Meanwhile, 3 other prints germinated and eventually led to . It's probably coincidence that my dud was also a B+, but I'd suggest get another spore print.

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cybernet said:
Put in incubator at 80 deg F and about 75% humidity.

Any suggestions appreciated.

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Don't do this.

They sound like a lost cause my man....

I know it's an old thread but ------> How long do shroom spores last?

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cybernet said:
Don't do what?

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cybernet said:
... Well those prints are the only ones I have. ...

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If you are inside the US, PM me your address and we can fix that problem.

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Wrightii said:
I once read a tek stating that closing your syringe (which you would have to make) with a lewer lock, and then wrapping a rubber elastic band around the plunger and lock would lead to a higher succes rate.
This would be because the added pressure from the elastic band would help hydrate the spores.
Never tried it myself, but thought it was interesting when I read it.
Maybe it helps!

Cheers,
-Wii

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