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InvisibleLegionOfShroom
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Registered: 02/13/17
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Cleaning Gasket Box Lids
    #27048212 - 11/19/20 11:59 PM (3 years, 2 months ago)

So I’ve recently acquired a bunch of 20qt and 54qt Sterilite gasket boxes. I’m just wondering how everybody sanitizes their totes after it’s time to toss out spent substrate. I’ve been using a sponge with soapy water for the bottom part of the containers. When it comes to the lids I’m hesitant to use soapy water because I don’t want it to get up in the gasket, which seams porous. Also the gaskets seam fragile so I wasn’t sure if it was ok to remove them so I could clean the lid with soap. I was considering just using rubbing alcohol on the lids for this reason.

It would probably be easier and save time to just wipe down the whole container with alcohol rather than soap, but I use the soap because I believe it’s more effective against certain contaminants than alcohol. There’s certain stuff that soap kills that alcohol doesn’t, right?

Any input would be appreciated :psychsplit:


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OfflineApolloBax
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Re: Cleaning Gasket Box Lids [Re: LegionOfShroom]
    #27048251 - 11/20/20 12:53 AM (3 years, 2 months ago)

Soap generally doesn't kill, it binds (the rinse removes what the soap binds.) You use soap to remove and alcohol to destroy. The first makes the seconds job easier. I don't have experience growing so I can just say from an infection control point of view, getting a porous material wet makes it more open to bacterial growth.


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InvisibleLegionOfShroom
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Re: Cleaning Gasket Box Lids [Re: ApolloBax]
    #27048257 - 11/20/20 01:10 AM (3 years, 2 months ago)

Thanks for that info, I obviously had some of of my facts mixed up. That’s really good to know. I’ll just be wiping down the lids with alcohol from now on. I’ll probably still use soap and water on the bottom halves to wash away the pieces of substrate


Edited by LegionOfShroom (11/20/20 01:13 AM)


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InvisiblebodhisattaMDiscordReddit
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Re: Cleaning Gasket Box Lids [Re: LegionOfShroom] * 1
    #27048359 - 11/20/20 05:01 AM (3 years, 2 months ago)

Soap kills and kills better than most other things. It rips microorganisms apart. Any soap is antibacterial soap but you should avoid antibacterial soap as it's not really much better than normal soaps.

Apollo has his his shit mixed up


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OfflineApolloBax
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Re: Cleaning Gasket Box Lids [Re: bodhisatta]
    #27048965 - 11/20/20 01:27 PM (3 years, 2 months ago)

From Meridian Health:
“Soap is the magic elixir that helps combine water and oil, breaking down the germs,” shares Monika Wolaniuk, D.O., family medicine specialist at Hackensack Meridian Medical Group. “Pin-shaped soap molecules have one end that bonds with water (the hydrophilic head) and the other end that bonds with oils and fats (the hydrophobic tail). When you build up a soapy lather, the molecules help lift the dirt, oil and germs from your skin. Then, rinsing with clean water washes it all away.”

From UC Santa Barbara
Ethyl alcohol kills bacteria mainly through 2 mechanisms: protein denaturation and dissolving the lipid membrane.

Proteins, the machinery of the cell, must be dissolved in water in order to properly function. When one puts a protein in ethanol (ethyl alcohol), the protein can not function properly and becomes denatured. Also, bacteria are surrounded by a lipid membrane (fatty acids). The membrane is held together because the alkane chain of a fatty acid is hydrophobic, and thus buries itself amongst other lipids. However, the lipids will freely dissolve in ethanol, causing a disruption of the bacterial membrane. This ruptures the bacteria so it can no longer live.

------------

Not all soap is antibacterial, but you aren't wrong about antibacterial soap being not a great option.
------

Soap is key part of removing bacteria and other debris but does not kill bacteria. Alcohol does kill bacteria although there are some bacteria which is resistant in spore form (ie Cdiff)

-----
Could go further into surgical scrubs which someone may consider a soap although it's not (ie chlorhexidine gluconate 4%) Chlorhexidine is great for long term reduction in bacterial loads on the skin although it to has some issues especially when stored in large containers as there are some organisms which can thrive in it.

Edit:

Bod's monotub tek uses gasgets so I know he would know much better how to clean them, but my shit wasn't mixed :heart:


Edited by ApolloBax (11/20/20 01:38 PM)


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InvisiblebodhisattaMDiscordReddit
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Re: Cleaning Gasket Box Lids [Re: ApolloBax]
    #27049109 - 11/20/20 03:15 PM (3 years, 2 months ago)

That's all correct but it's not the full picture. Soap kills just as well as alcohol but soap also washes microorganisms away.

Scrub soap is soap with chlorhexidine  not just the chlorhexidine
Betasept is probably the most common surgical scrub soap around.


Both betasept and ethyl alcohol can become contaminated with microorganisms that have resistance to them.

I don't clean my gaskets. I took them out and they're in a box for use later if I ever decide to use the tubs as tubs for stuff that isn't mushrooms


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Invisiblemushhead
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Re: Cleaning Gasket Box Lids [Re: bodhisatta]
    #27049152 - 11/20/20 03:34 PM (3 years, 2 months ago)

:takingnotes::shrug:


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OfflineApolloBax
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Re: Cleaning Gasket Box Lids [Re: bodhisatta]
    #27049180 - 11/20/20 03:46 PM (3 years, 2 months ago)

Those are not soap...

From the FDA:
What’s the regulatory definition of soap?

Whether a product is a “soap” in the traditional sense, or is really a synthetic detergent, helps determine how the product is regulated. So, let’s take a look at how “soap” is defined in FDA’s regulations;

To meet the definition of soap in FDA’s regulations, a product has to meet three conditions:

What it’s made of: To be regulated as “soap,” the product must be composed mainly of the “alkali salts of fatty acids,” that is, the material you get when you combine fats or oils with an alkali, such as lye.
What ingredients cause its cleaning action: To be regulated as “soap,” those “alkali salts of fatty acids” must be the only material that results in the product’s cleaning action. If the product contains synthetic detergents, it’s a cosmetic, not a soap. You still can use the word “soap” on the label.
How it's intended to be used: To be regulated as soap, it must be labeled and marketed only for use as soap. If it is intended for purposes such as moisturizing the skin, making the user smell nice, or deodorizing the user’s body, it’s a cosmetic. Or, if the product is intended to treat or prevent disease, such as by killing germs, or treating skin conditions, such as acne or eczema, it’s a drug. You still can use the word “soap” on the label.

--

Just wanted to add: Bod you obviously have the practical applications down. Your teks are great. You've spent a ton of time doing this and it shows. All respect to you.


Edited by ApolloBax (11/20/20 03:58 PM)


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