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Shop: Mushroom-Hut Liquid Cultures   Unfolding Nature Unfolding Nature: Being in the Implicate Order   North Spore Injection Grain Bag

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OfflineBobT
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Registered: 08/01/20
Posts: 2
Last seen: 1 year, 5 months
WBS & LC
    #27048015 - 11/19/20 08:13 PM (3 years, 2 months ago)

Hi All,

I got a PE syringe and tried to do some WBS tek and liquid culture experiments with it. Needless to say I'm very very new at this. Anyway, there is no sign of life in the WBS jars (4), but 2 out of 3 LC recipes are looking good!
Question is: Can I use the same WBS jars that have been sitting around for 28 days or should I make a new batch to test out the LC and how much LC per QT jar!?
I soaked the WBS over night with coffee and gypsum, and brought to a boil before trying to dry which took forever, jarred & PC'd.

TIA


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Offlineverum subsequentis
seeker of truth
I'm a teapot User Gallery


Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 7 months
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Re: WBS & LC [Re: BobT]
    #27048050 - 11/19/20 08:43 PM (3 years, 2 months ago)

You could use them but it's not as safe as making new ones. Some bacteria could easily have taken hold while they've been chilling. If you want you can open one up and smell it. It'll be toast after that but if it still smells like wbs (as opposed to anything sweet or sour) and it doesn't have any visible things growing, the rest are probably still ok.

Having said that, I'd just make new jars.

How much LC depends on a lot of things. Are you pouring or using syringes? Easiest is just a quick splash via free pour.


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OfflineBobT
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Registered: 08/01/20
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Last seen: 1 year, 5 months
Re: WBS & LC [Re: verum subsequentis]
    #27048393 - 11/20/20 05:46 AM (3 years, 2 months ago)

I was thinking of using a syringe so that the rest of the LC is still somewhat viable...


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Offlineverum subsequentis
seeker of truth
I'm a teapot User Gallery


Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 7 months
Trusted Cultivator
Re: WBS & LC [Re: BobT]
    #27049301 - 11/20/20 05:18 PM (3 years, 2 months ago)

Doesn't matter if you use an LC or not. You can contam it either way. In all reality using a needle can cause contams more readily than pouring. Again, either way you may accidentally poop in your LC. It's just that you can't be sure that you've got a clean SHIP unless you just removed it from the cooker and have handled it properly. Otherwise you are relying on ISO and ISO doesn't sterilize. It does a good job of sanitizing but there's still a possibility that you'll push a nasty straight through the ship.

That's why the term "noob needle" exists. Done properly you can open and pour an LC without contamming it 100% of the time.


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Shop: Mushroom-Hut Liquid Cultures   Unfolding Nature Unfolding Nature: Being in the Implicate Order   North Spore Injection Grain Bag


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