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Offlineneonero
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Comments on this G2A
    #27046996 - 11/19/20 11:49 AM (8 days, 20 hours ago)

Hi,

I transferred rye grains to agar. The grain looks contaminated, but that´s not what I was thinking about. It is all the white spots.
I used a SAB. Took one grain with sterilized scalpel. Placed it in center of dish.
You think the white spots is myc or contam?


Edited by neonero (11/19/20 01:03 PM)


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OfflineHikeadellic
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Re: Comments on this G2A [Re: neonero]
    #27047005 - 11/19/20 11:54 AM (8 days, 20 hours ago)

Your picture did not go through. If you are concerned about contams on your plate you should just keep transfering until its a clean plate


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Offlineneonero
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Re: Comments on this G2A [Re: Hikeadellic]
    #27047142 - 11/19/20 01:04 PM (8 days, 19 hours ago)

Should be fine now. Just beginners fault:-)


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InvisibleSunny Skies
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Re: Comments on this G2A [Re: neonero]
    #27047274 - 11/19/20 02:26 PM (8 days, 18 hours ago)

you have 4 plates of trash my friend..bin them.


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Re: Comments on this G2A [Re: Sunny Skies]
    #27047318 - 11/19/20 03:00 PM (8 days, 17 hours ago)

Quote:

Sunny Skies said:
you have 4 plates of trash my friend..bin them.




Thank you.

What do you think the white spots are?

You see, the jars look fine. All white.


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Re: Comments on this G2A [Re: neonero]
    #27047996 - 11/19/20 10:02 PM (8 days, 10 hours ago)

Take a closer pic of the growth on the plate on the top left.

Why are you going to grain to agar? You are supposed to go agar to grain.


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Re: Comments on this G2A [Re: CocaineBuffet]
    #27048057 - 11/19/20 10:48 PM (8 days, 9 hours ago)

People have had success going grain to agar to agar / grain. Sometimes its a pain in the ass to germinate spores on a plate so its easier to just start with myc. Could also argue that a single grain has less competition between sub strains so it could make sense to start from that


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Re: Comments on this G2A [Re: CocaineBuffet]
    #27048320 - 11/20/20 05:45 AM (8 days, 2 hours ago)

Quote:

CocaineBuffet said:
Take a closer pic of the growth on the plate on the top left.

Why are you going to grain to agar? You are supposed to go agar to grain.



Thank´s for asking. I am trying to learn to grow mushrooms, so I guess it is part of the learing process.
First I did S2A, then A2G. Grain to minitub. It contaminated with green mold. Then, I did S2A->A2G. Jars are ready now, so I wanted to see if it would be possible to transfer myc from jars to Agar thinking it would be faster. I also only have one print and I can´t get any a new one right now.

Here is a closer look at the top left dish:


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InvisibleCosmicKramer
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Re: Comments on this G2A [Re: neonero]
    #27048666 - 11/20/20 11:54 AM (7 days, 20 hours ago)

You might get lucky if you take a tiny bit from the 12 o'clock location on the center blob. Try taking your transfers earlier, as soon as you can identify myc and before the mold gets all over everything.  After 3-4 transfers, you may get things cleaned up. 

When you transfer, those satellite spots are always contams, I would pitch the others.  You mentioned your jars are all white, chances are high that those are full of mold.  Can you post pics of the jars?


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Re: Comments on this G2A [Re: CosmicKramer]
    #27049018 - 11/20/20 04:00 PM (7 days, 16 hours ago)

Quote:

CosmicKramer said:
You might get lucky if you take a tiny bit from the 12 o'clock location on the center blob. Try taking your transfers earlier, as soon as you can identify myc and before the mold gets all over everything.  After 3-4 transfers, you may get things cleaned up. 

When you transfer, those satellite spots are always contams, I would pitch the others.  You mentioned your jars are all white, chances are high that those are full of mold.  Can you post pics of the jars?




I have checked the jars better, and there is mold in the jar.

I will trash these dishes, and start over with spores to Agar.


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InvisibleCosmicKramer
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Re: Comments on this G2A [Re: neonero]
    #27050327 - 11/21/20 01:19 PM (6 days, 19 hours ago)

That sucks.  It happens to the best of us.  Keep on trucking and you will get there.


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Re: Comments on this G2A [Re: CosmicKramer]
    #27050417 - 11/21/20 02:42 PM (6 days, 17 hours ago)

Quote:

CosmicKramer said:
That sucks.  It happens to the best of us.  Keep on trucking and you will get there.




Thank´s for the encouragement. First time failed. Started over with S2A.


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