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alaskappalachian
Entitiologist


Registered: 10/22/19
Posts: 1,688
Loc: The 49th Dimension
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Panaeolus Cinctulus Grow 4
#27045405 - 11/18/20 11:45 AM (3 years, 2 months ago) |
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CINCTULUSI wanted to grow these to a. satisfy a nostalgic want to see them again (I grew up in the Carolinas and it was one of the only actives there, and I lived out in horse country...), and b. to start working on accumulating sufficient pseudosclerotia from them for bioassay. Given that my environment is truly awful for growing panaeolus in the winter (woodstove and super low temperatures at night), and that I can't run a proper chamber or Martha (off grid/limited electric), I questioned rather I was going to get anything going. However: -(date unknown): inoculated 4 plates/prepours (5g brf/9g agar/500ml H20). Lost a batch of photos to a bad SD card but suffice to say, myc looked like typical pan myc (wispy). - Myc growth was fast in all cases. Took 3 days for visible germination to present. Made a LI from a uniform-looking T3 and inoculated 6 extra brf cakes I'd been sitting on, a couple pt jars of wbs, and a pint of wbr. Growth was fast and jars/mediums were finished colonizing completely (including a short consolidation period for the cakes) within 2 1/2 weeks (approximately). Interestingly, the cakes and assorted jars finished almost exactly at the same rate. -One wbs jar developed a patch of aspergillus and was discarded. The wbr was left to colonize. The remaining wbs jar was spawned to a couple quarts of steer manure/compost and shredded grass stalks from big patches of coastal grasses that I use to grow oysters on- in a 6qt, and left with the lid snapped on to colonize. Extremely fast colonization even with nighttime temperatures in the room dipping into the low 50s a couple times and regularly being 60ish at night (five days to 100% colonization). -This was cased with a mix of potting soil and a little leftover peat (both pasteurized in jars @ ~160for 1.5hrs).  Paltry harvest but enough to snag a bunch of prints and dose. Ayyyye  No weight recorded but if I remember I'll get a dry weight. Wbs jars were beginning to produce sclerotia (just a few) before their divergent evolution(s), but for whatever reason, the brf cakes really were some pseudosclerotia producing fuckers. They started producing them @ ~70% and are still doing so, although myc has sort of... grown around them a bit as they have consolidated for like a couple weeks (just observing them...).  Gonna spawn these and collect the sclerotia tomorrow after I pasteurize some more sub for them. (One jar had completely different growth and no stones btw so going to spawn it to one of my small containers and see what it produces for science. Just a fun little grow. Figured I would share with you guys before I scale up with these. Gonna do a big, underbed container next.
Edited by alaskappalachian (11/18/20 12:16 PM)
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mushhead
Potato Devourer



Registered: 08/22/14
Posts: 2,218
Loc: Dimension H-231
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 I'm waiting for a shipment of some wedges which hopefully includes Pan Cints.
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alaskappalachian
Entitiologist


Registered: 10/22/19
Posts: 1,688
Loc: The 49th Dimension
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Re: Panaeolus Cinctulus [Re: mushhead]
#27045454 - 11/18/20 12:14 PM (3 years, 2 months ago) |
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Nice. Anxious to see how that goes for you. If you don't end up with a wedge and want a print to fuck with, let me know.
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mushhead
Potato Devourer



Registered: 08/22/14
Posts: 2,218
Loc: Dimension H-231
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I'll take a print off your hands just to have it as a backup. My local climate only has Pan Cints wild here and only within the spring. I wasn't able to find any this year.
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A.k.a
Stranger



Registered: 10/27/19
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Re: Panaeolus Cinctulus [Re: mushhead]
#27045633 - 11/18/20 02:16 PM (3 years, 2 months ago) |
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Cincts are so cool looking.
How much did you need to eat? Half the stuff I read says they’re super weak and half says people just think that because they tend to grow next to near identical non active pans.
Did you just fruit those in regular shoeboxes with nothing special like a fogger??
Also how’s the trip?
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LAGM2020     
Edited by A.k.a (11/18/20 02:18 PM)
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alaskappalachian
Entitiologist


Registered: 10/22/19
Posts: 1,688
Loc: The 49th Dimension
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Re: Panaeolus Cinctulus [Re: A.k.a]
#27045662 - 11/18/20 02:36 PM (3 years, 2 months ago) |
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When I used to eat them from wild collections (both from collections in NC/SC and in Oregon) they were consistently roughly as potent as cubensis (but a little cleaner). I've gotten weak collections but rarely. I too am of the opinion that most people who don't have particularly memorable experiences with pan cincts misidentify part or all of their collection. I'll be trying these maybe Friday night. As far as maintaining humidity and/or surfaceconditions... man do I have a hard time with that with something like a shoebox this time of year. Gets too cold at night (pretty cold sometimes- like 48-50) which interferes with maintaining humidity for something like pans; but by dubbing it and adding a little LED in a clamp light right on top of the box you get outstanding humidity with the corners cracked for FAE. (My SGFCs just don't work right either during coldest months in my house, and they're all full anyway)...
  Ghetto looking AF but works great. I usually use bigger tubs this time of year because of the temperature fluctuations. They are instrumental in combatting that nightly flatline and the constant woodstove drying. Lights would help a ton, but I just can't spare the power believe it or not. Everything other tub size runs fine but shoeboxes and 16 qts not so much. For growing "real " pans I am putting together a little enclosure using PVC and painter's plastic to keep a 5 gal pot of stove water in overnight underneath... Last two attempts were a no-go,so had little hope for these initially...
Edited by alaskappalachian (11/18/20 04:56 PM)
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A.k.a
Stranger



Registered: 10/27/19
Posts: 16,816
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Right on.
Sounds like you need one of these. https://newatlas.com/good-thinking/solar-powered-autoclave/
Conserve power and heat the room lol.
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LAGM2020     
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bobwastaken
under construction


Registered: 06/17/10
Posts: 1,971
Loc: SA
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Re: Panaeolus Cinctulus [Re: A.k.a] 2
#27046035 - 11/18/20 06:01 PM (3 years, 2 months ago) |
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Very nicely done alaskappalachian. I'd like to play with this species in future as well. I wonder about the potency since some species can be markedly more potent when cultivated compared to their wild cousins. Take P.alutacea for one example. Not only are cultivated specimens much more robust than wild but potency is comparable to P.subaeruginosa. Wild collections wouldn't exceed your average P.cubensis.
Edited by bobwastaken (11/18/20 07:37 PM)
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alaskappalachian
Entitiologist


Registered: 10/22/19
Posts: 1,688
Loc: The 49th Dimension
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Hey thanks Bob! I'll take a compliment from you any day. I am very curious how potency is going to pan out as well. Always right on par with cubes from the wild IME and these didn't display bruising, but I definitely have collected active cincts that didn't bruise at the base (several times). Might try some Saturday morning and take a nature walk... Probably doing a trip report if it...wait for it... pans out lol and they are decent. I'll be experimenting with the sclerotia for sure as well. Finally pasteurizing some manure tonight to spawn those cakes and see how much of those I have... Also that's cool info on the alutacea btw. Wondered how active those were...
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Baba Yaga
♥ coir grower

Registered: 09/13/20
Posts: 4,035
Loc: Hyperspace Chicken Coop
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I just quoted this thread again. It's such a neat and beautiful grow.
Deserves a bump.
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HappinessStan
Fungivore



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Re: Panaeolus Cinctulus Grow [Re: Baba Yaga]
#27952727 - 09/16/22 11:35 AM (1 year, 4 months ago) |
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Now I wanna grow pan cincts, ffs.
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Cob
Ovoid fanboy


Registered: 07/01/22
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Loc: East Coast, USA
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Re: Panaeolus Cinctulus Grow [Re: Baba Yaga]
#27952767 - 09/16/22 12:05 PM (1 year, 4 months ago) |
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Quote:
Baba Yaga said: I just quoted this thread again. It's such a neat and beautiful grow.
Deserves a bump.
Hell yeah, these are another one on my list because they grow in the wild here. Would love to get my hands on one or a print. I didn't know anyone had grown them.
I've been hunting and haven't found anything yet. I surely will eventually find some and be able to attempt some like above.
Good bump Baba Yaga.
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Panny
Voyager



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I'm wondering if anyone else here has cultivated these since.
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