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OfflineEarthling
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Ok, am I growing mycelium or something else * 1
    #27043821 - 11/17/20 12:45 PM (3 years, 2 months ago)

So one of my earlier posts I had an issue in the PF jar with black mold and I'm worried that may be what I've been playing with since 😅 Having read the contamination guides on here after wondering when the heck I'm gonna reach the end point of harvest, I can see some contams look very similar to myc.

SOS 😂

I've been trying to clean up what's on agar (MEA store bought) and am at T7-T8 now. I'm starting to think I may not be cultivating mushrooms 😅 I said wth and sent a few qts of clean looking grain into shoe box and mini mono's, to see what happened, ideally get a teeny flush or even abort lol (to confirm I actually have shrooms) but those haven't gone anywhere to date, partially colonise then trich got in. I have two still ok but not doing anything. Coir and verm mix. I've worked with PF, grain, agar and LC so far. I began at spore print and this is my first time cultivating and working with cubes.

Out of about 80 odd plates I've transferred so far I've had three contam with bac (one is in the photo), a couple got some dark mold like contam on edge of petri. Working in a SAB and I PC for 45mins, pre soaked grain always. I've tried transferring at around five days when it's just beginning to run, I've also left some plates for three weeks to see if contam will pop before I take from them. The jar took about 3wks to fully colonize, I've included it as it's just begun to excrete metabolites the last few days, I'm hesitant to expand it tbh. The plates are examples of what the myc growth looks like and the black patches that seem to be 'my issue'. Front and back pics of three plates. If I transfer off that I nearly always notice the black quickly colonizing on top before being overrun by white. I have a bunch of fully colonized plates I can take pics of it need be.

Any insight would be fab, links or further reading is cool, tips of how to over come or start again with new culture etc.



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Offlinefuneralfather
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Re: Ok, am I growing mycelium or something else [Re: Earthling] * 1
    #27043981 - 11/17/20 02:35 PM (3 years, 2 months ago)

I’m an absolute noob at this I’m just now getting my first pins. But if I have learned anything. Black is not what your looking for.  My guess given how common it was for me is that you got cob web in between but again I’m only responding to give this more views so others will chime in. Best of luck buddy.


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OfflineFree Dive
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Re: Ok, am I growing mycelium or something else [Re: funeralfather]
    #27044044 - 11/17/20 03:19 PM (3 years, 2 months ago)

Only the second picture looks normal to me. The jar looks normal too, the yellowing in common from excess water. See if you can get a fruit to clone before any infection is apparent.

The rest of the plates have very unusual looking growth and would make me think you are growing something else.

I would take 2-4 transfers of the second petri dish at about 2 and 3 o'clock (bottom right) and post pictures after 1-2 weeks of growth. Hopefully at least one will be clean.

I would also try a new spore print to agar.
-Scrape a very small amount and try to have the spores land near the middle of the agar plate.
*Review SAB tecs and try to reduce your contamination ratio.


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OfflineEarthling
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Re: Ok, am I growing mycelium or something else [Re: Free Dive]
    #27044126 - 11/17/20 04:06 PM (3 years, 2 months ago)

Thanks guys. Second pic is top of first pic, three plates total top and bottom there.

I took a transfer from it a few weeks back, can just see the slice at 6 in first pic. That now looks like the other two (younger) plates and has dark on it also. The grain culture that may be ok was a LC made from a transfer from one of the cleaner agar plates a while back (which later showed up this contam) so that could be in the LC and now that grain. I've just done an agar test of the LC to see where it's at.

SAB tech runs from cleanest to dirtiest plate at present, flame before each transfer, try not to hover or drag through air to new plate. Lots to learn, many points to go wrong 😅

Should note the OG culture was MS, never had an isolate so assume these are all MS too.

Have lots of other fully colonized plates if additional pics are useful. Want to push forward to try get a fruit to transfer but the tubs are stalling, pointless if I'm growing mold 😅

Will add some pics of extra greeblies and some experiments from duds.


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OfflineEarthling
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Re: Ok, am I growing mycelium or something else [Re: Earthling]
    #27044204 - 11/17/20 04:35 PM (3 years, 2 months ago)



A range of pics that show the traits of whatever it is I'm culturing. It likes to grow up. The black contam usually stays put like second pic of several plates until it's a good 3-4 weeks old then it must get to the edges of the plate as I see them change first then it seems to slowly take over. The patchy look comes in around that time. I've included some of those pics too. Just various stages and substrates of the same cultures.

Many of these were experimental, the cutting of the middle contam out and the agar wedge straight onto grain that wasn't clean to begin, I figured I could at least play around with the duds 🤷‍♀️

Buuuut if this all mold well, I'll be laughing and crying 😂🤞 I'm hopeful it's multispore traits and I just gotta step up the SAB and transfer game!


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OfflineFredsGotSlacks
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Re: Ok, am I growing mycelium or something else [Re: Earthling]
    #27044313 - 11/17/20 06:01 PM (3 years, 2 months ago)

Hey Earthling,
I haven’t got a lot to contribute, but I think I was growing something along the same lines as yourself.
Link to my thread here
The consensus was that it wasn’t mycelium, which I conceded to just tossing and that was from verm and D3monic, 2 pretty well known guys are the forums, not to mention the others who helped out too.

Mine also had black spots in the middle after a few weeks but the outside was slightly throwing me off because it wasn’t fast growing (which I’ve read mold grows faster then mycelium).  It wasn’t quite tormentose or rhizo growth so it had me really guessing.  Hence creating a post about it.

What is your agar recipe that you’re using??


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OfflineEarthling
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Re: Ok, am I growing mycelium or something else [Re: FredsGotSlacks]
    #27044352 - 11/17/20 06:27 PM (3 years, 2 months ago)

Thanks for your reply! I can see your confusion there too, mine looks a bit different but along similar lines of being 'not quite right'. I can't yet judge its speed as I don't have a comparison under my belt yet, I have notes somewhere on how long to reach a full plate if that helps.

I took the advice of if you have nothing else and nothing to lose send it and see what happens. Not much so far 😅

The agar plates are pre made MEA from a local lab, I am just learning how to make my own now.

What a great few months culturing who knows what, I sure learnt a bit 😂


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OfflineCocaineBuffet
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Re: Ok, am I growing mycelium or something else [Re: Earthling]
    #27044384 - 11/17/20 06:40 PM (3 years, 2 months ago)

That grain shot is shot. Standard PC cycle is 1.5 hours.

I don't like any of those plates. Where did the spore print come from? How did you add it to the agar? How old is it?

You are right in being hesitant in expanding any of that.


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InvisibleRafiikii
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Re: Ok, am I growing mycelium or something else [Re: CocaineBuffet]
    #27044477 - 11/17/20 07:41 PM (3 years, 2 months ago)

Quote:

CocaineBuffet said:
That grain shot is shot. Standard PC cycle is 1.5 hours.

I don't like any of those plates. Where did the spore print come from? How did you add it to the agar? How old is it?

You are right in being hesitant in expanding any of that.




exactly

looks all bad to me.


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Offlinedirtybirdx
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Re: Ok, am I growing mycelium or something else [Re: Rafiikii]
    #27044507 - 11/17/20 07:57 PM (3 years, 2 months ago)

Yikes...honestly I’ve never even come across some of the stuff I see there and I’ve done hundreds of plates.

With agar and transferring - the idea is to get a piece of mycelium transferred as quickly as you can get it.  You’re outrunning the contams.

There shouldn’t be a reason for you to be examine plates that have grown out for so long.

I take another transfer after 4-6 days.  You can always use the old plate afterwards, letting it grow out, transferring from it again, or using it as inoculate if it stays clean.

Take a peek at the pic in my sig - no reason why your cultures can’t look like that with a bit of tweaking, especially if you’re doing the basics of sterile tek.

The yellow in your jar is metabolites.  If it was just a tiny bit here or there you could ignore it and spawn, but that’s a lot.  It will create a bunch of metabolites after you spawn, likely an overlay (aggro mycelium growth beyond sub colonization), and probably a shitty pinset with a bad yield.  Also more prone to other contams.

Chunk it and aim for cleaner spawn otherwise you’ll be asking us in 3 weeks why your tub isn’t pinning and everyone will say ‘you didn’t have clean spawn’.

Keep on trucking.  Everyone has to get tripped up a bit before they figure it out. 

👍


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OfflineEarthling
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Re: Ok, am I growing mycelium or something else [Re: dirtybirdx]
    #27044605 - 11/17/20 09:18 PM (3 years, 2 months ago)

Well that seals it. Cheers all.

Spore print was sent, made a syringe and went to PF. I put some spores on agar and they did nothing. I took og of this culture from a PF jar (substrate) that I thought was clean when I first cracked jar to birth, but wasn't (if you got nothing else make a backup if what you do right). Once it was on agar I've tried to transfer to clean up. Yep, I'm new! I assume what I transferred out wasn't mycelium, or it was and got overthrown and because I'm noob I didnt see it wasn't growing right, I put it down to being a multispore 🤦‍♀️😅

The fully colonized plates are old indeed, just stored to see if they stayed clean (hah!!). I ended up transferring the youngest plates around day five from their parent, glad to know am on the right track there with timing. The first few partially colonised plates in OP are about a week past the stage I would usually transfer from, I haven't transferred from them and won't now. I added the old pics in various stages to show what was going on as culture aged, figured it would assist the masters, scared them with newbie culture goofups instead maybe 😅

The metabolites have just started cranking in the last few days, that jar was full col a few weeks back now but I've been too scared to touch it. Would a healthy jar stay metabolite free for an extended period once colonised?

Well shit me, 1.5 hours for grain in PC!? I have read all over the place about 45min at 15 PSI, has my reading gone down the wrong path? Pre soak 24hrs, precook, then jar, PC cools overnight. It has been sufficient and successful for my Oysters but I am more than happy to revise if it will assist my efforts.

Ahhh the reading I know I'm about to get into as I bank this one to experience, thanks again all! Think I'll start with a new plate, slant or LC from a vendor so I know I'm already running what I intend to, I'll come back to the spores if I ever get a grow 😜


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InvisibleRoger Clemency
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Re: Ok, am I growing mycelium or something else [Re: Earthling]
    #27045043 - 11/18/20 08:12 AM (3 years, 2 months ago)

I don’t know if that’s cube myc in the jar at all man. It’s hard to say but I really don’t like the look of it.

45 min is sufficient for 1/2 pint BRF cakes in the PC. For grains 90 min minimum at 15 psi. I usually stay around 17-18 psi for 90-120 min depending on what’s up. Also make sure you vent the PC for 8 minutes before adding the weight.

And like someone mentioned before, spores to agar, transfer once you have adequate growth, then your next transfers should be within a week. Probably 5 days or so and you transfer a little wedge from the leading edge of new growth.


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