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tripster30
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First time with agar
#27041966 - 11/16/20 09:50 AM (3 years, 2 months ago) |
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I started 6 jars of agar .. I'm wondering if it's possible to tell me how it's looking from these pictures and where I should go from here .. I mainly just trying to get mycelium grown out so I can put it to Rye Berry jars 
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tryptkaloids
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Re: First time with agar [Re: tripster30]
#27042024 - 11/16/20 10:32 AM (3 years, 2 months ago) |
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There's a ton of bacteria on that plate as well as myc In order to get anything from that you'll need to make several transfers from healthy tissue.
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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tripster30
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Well that sucks I wasn't really trying to get into sectoring or transferring .. I used a Spore syringe that I had done brown rice flower cakes with had 2 mls left So thought I would give it a try.. Guess it was a failure
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tryptkaloids
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Re: First time with agar [Re: tripster30]
#27042082 - 11/16/20 11:11 AM (3 years, 2 months ago) |
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You can still cut that myc and put it to clean agar.
Agar's main use is cleaning cultures up, you have mycelium there, it'll just be a little work to make it usable.
I wouldn't call it a failure, I'd call it practice and source new spores for backup.
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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Zsuzsi
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Re: First time with agar [Re: tripster30]
#27042086 - 11/16/20 11:15 AM (3 years, 2 months ago) |
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Quote:
Guess it was a failure
Wait, how was it a failure ? from what I see (bear in mind I'm far from experienced and the use of jars + pic quality doesn't help to make sure), your myc looks healthy here.
I believe what the person answering you meant is that spore syringes are inherently dirty and as a result, there are almost certainly some bacteria in those jars that you can't necessarily see.
Your jars aren't a toss; you just want to take transfers to other agar dishes/jars from a small part of the mycelium in those jars (take a small wedge from close to the leading edge of your myc but still inside the myc; deposit said wedge into the center of a new jar/dish; do that in a sab).
Once you've done that at least twice, then the chances of contamination are vastly lowered and if the myc then looks good, you can ago ahead an inoculate grain (with a similar process as your transfer but a much bigger wedge).
You can do more than two transfers, and it's not a bad idea to do enough so the myc is a bit less fluffy since that fluffiness can apparently help some contams to hide inside it. With that being said, since you're using jars (I imagine with no pour ?) I suppose you'd have a hard time seeing contams 100% - or at least a harder time than with a dish - since you don't have a handy transparent lid to see through.
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tryptkaloids
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Re: First time with agar [Re: Zsuzsi]
#27042092 - 11/16/20 11:18 AM (3 years, 2 months ago) |
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I meant around the myc.
Orange isn't a good thing. Especially on agar
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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Zsuzsi
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Isn't that just some agar that was poured a bit haphazardly and solidified there ? hence the slightly lighter color ? the color is close to the agar color itself, and the second pic made me think it was (look at the closest part of the glass to the camera; that thing is stuck to the glass and raised a bit above the general level). I've seen a similar effect visually when overpouring dishes.
Edited by Zsuzsi (11/16/20 11:26 AM)
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tryptkaloids
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Re: First time with agar [Re: Zsuzsi]
#27042103 - 11/16/20 11:27 AM (3 years, 2 months ago) |
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Look at the 3rd pic, there's a clear separation between the agar and bacteria. I see it, even if you don't. Surely if op decides to transfer (which he should) he will see it when he opens it up
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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Zsuzsi
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Re: First time with agar [Re: Zsuzsi]
#27042105 - 11/16/20 11:28 AM (3 years, 2 months ago) |
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The more I look at it and the more I think you're right, my apologies.
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tryptkaloids
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Re: First time with agar [Re: Zsuzsi]
#27042110 - 11/16/20 11:30 AM (3 years, 2 months ago) |
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Don't be sorry, be silly! I have more experience with bacteria than I like to admit
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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coversall
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Re: First time with agar [Re: tripster30]
#27042120 - 11/16/20 11:34 AM (3 years, 2 months ago) |
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Quote:
tripster30 said: I wasn't really trying to get into sectoring or transferring
If starting agar from spores then at least one transfer is an important part of the process as the main aim to to use the agar to separate the myc from the bac/mold!
Give it a whirl!
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tripster30
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Re: First time with agar [Re: coversall]
#27042159 - 11/16/20 12:05 PM (3 years, 2 months ago) |
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Thank you for everyone's responses definitely brand new to all of this.. So the orange streaks are bacteria? Could they have been caused by putting to much Spore solution on to the agar and then it running around the jar? Was doing no pour and figured if using a Spore syringe I would never have to open the door once until I needed to
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Zsuzsi
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Re: First time with agar [Re: tripster30]
#27042165 - 11/16/20 12:10 PM (3 years, 2 months ago) |
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Jesus christ they're looking fucked up. It's ok though, as the people above have said just transfer a bit of the myc (near the edge but not that near - stay away from the orange monstrosity) to new plates.
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tryptkaloids
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Re: First time with agar [Re: Zsuzsi]
#27042372 - 11/16/20 02:56 PM (3 years, 2 months ago) |
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I'm lazy rn, but try to find How it should and shouldn't look And Cleaning and isolation on agar
Those threads should help
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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verum subsequentis
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Trypt is right. Your syringe is most likely riddled with bacteria. From the looks of the myc id guess it'll take quite a few transfers to clean.
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tripster30
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I injected 8 brown rice flour cakes and they all colonize without contamination so I figured this ment it was clean but now from what I'm understanding just because the cakes came out doesn't mean there's not contamination in the syringe somewhere.. could be wrong but I think a big part of it is the liquid solution rolling around in the jar instead of one drop staying in the center until it's soaked in.. But I could be wrong
Edited by tripster30 (11/16/20 04:29 PM)
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tryptkaloids
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Re: First time with agar [Re: tripster30]
#27042505 - 11/16/20 04:33 PM (3 years, 2 months ago) |
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Water doesn't cause bacteria. Bacteria causes bacteria. Water helps it move.
Its possible your syringe was relatively clean and you contaminated it during use
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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verum subsequentis
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Re: First time with agar [Re: tripster30]
#27042507 - 11/16/20 04:35 PM (3 years, 2 months ago) |
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(beat me to it)
BRF cakes are famous for doing well even with bacterial syringes. Which is nice because a lot of "professional" syringes are bacterial.
Too much spore solution isn't the best but it doesn't create bacteria. How could it? There are only so many options. Either the syringe was dirty or you somehow introduced the bacteria while inoculating.
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tripster30
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Everyone said don't be intimidated by agar.. But it seems I'm far from understanding it LOL.. I had better luck buying multi Spore syringes from vendors and just injecting it into my grain jars but I thought going to agar would make the process better somehow
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tryptkaloids
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Re: First time with agar [Re: tripster30]
#27042530 - 11/16/20 04:53 PM (3 years, 2 months ago) |
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It does make it better. It keeps loads of us from wasting materials on shitty syringes that can be a crapshoot
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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tiptrippy
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Re: First time with agar [Re: tripster30] 1
#27042532 - 11/16/20 04:54 PM (3 years, 2 months ago) |
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Quote:
tripster30 said: Everyone said don't be intimidated by agar.. But it seems I'm far from understanding it LOL.. I had better luck buying multi Spore syringes from vendors and just injecting it into my grain jars but I thought going to agar would make the process better somehow
Have you spent at least a few hours reading and researching all about agar and its uses in the forums here? You can't get overwhelmed and give up if you don't even give it a proper try. Petri dishes will make you're life much easier. Working with agar isn't contamination free, it helps you process cultures by transferring from dish to dish to eventually get that 100% contamination free culture. You can't just expect to put spores on agar and then go straight to grain. Do some reading if you care. Or just stick to MS and BRF
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tripster30
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I've seen the good working with agar can do which is why I got interested in it it appears I just still have a lot to learn.. Is it possible to get a syringe from a vendor put it to agar.. it grow out and then be able to put it to grain or it just doesn't work that way?
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tryptkaloids
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Re: First time with agar [Re: tripster30]
#27042547 - 11/16/20 05:02 PM (3 years, 2 months ago) |
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Its possible to just go straight to grain with it, but its less safe than taking a transfer or 2
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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tiptrippy
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Re: First time with agar [Re: tripster30]
#27042549 - 11/16/20 05:04 PM (3 years, 2 months ago) |
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Quote:
tripster30 said: I've seen the good working with agar can do which is why I got interested in it it appears I just still have a lot to learn.. Is it possible to get a syringe from a vendor put it to agar.. it grow out and then be able to put it to grain or it just doesn't work that way?
No. Usually you will get the syringe, put 1 drop on a plate and streak it, wait for growth, it will probably be contaminated (which is fine), transfer a piece of healthy growth (AWAY FROM THE CONTAMS) to a brand new plate of agar and you should get a cleaner culture from that. After 1 or 2 transfers it should be healthy enough for grain.
From this single question you asked it seems like you haven't done the reading or you just don't fully grasp the point of agar.
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verum subsequentis
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Re: First time with agar [Re: tripster30]
#27042551 - 11/16/20 05:06 PM (3 years, 2 months ago) |
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It is possible. Just depends on whether or not it was made with care.
The benefits of agar are myriad but not needed if one is happy with sticking a needle into a brf cake and getting a few fruits. If, however, you want to a. never have to buy another syringe b. know that you have clean cultures c. know that you can clone and save any genetics you come across d. Know that at any moment in time, iffin you get a wild hair up your ass, you can pull a slant out of the magic fridge and be swimming in magic in no time
Agar is for you. It isn't magic. You still have to learn and do things right.
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tripster30
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I am definitely interested in all of that! Thank you guys for the info .. off work in a couple of days and going to try to transfer some of the healthier looking mycelium to fresh agar and see how that goes.. Is it better to start with a spore print instead of Spore syringe or is it pretty much the same results either way..? I have lots of Spore prints the only reason I went from syringe was trying to do the no pour thing and not have to open the jar
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verum subsequentis
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Re: First time with agar [Re: tripster30]
#27042573 - 11/16/20 05:19 PM (3 years, 2 months ago) |
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Spores in syringes are hydrated so they have that going for them. If they are clean. Prints have better chances of getting clean growth even if the print has a little nasty on it because there is no liquid spreading the nasty all over the show.
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IdiotCircusBoy
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Quote:
tryptkaloids said: I'm lazy rn, but try to find How it should and shouldn't look And Cleaning and isolation on agar
Those threads should help
This post is literally called "How it should look." Lots of great examples.
https://www.shroomery.org/forums/showflat.php/Number/17231150#17231150
and this one is literally called "Stro's Cleaning and Isolating On Agar"
https://www.shroomery.org/forums/showflat.php/Number/18430998#18430998
-------------------- Just call me Idiot "People hasten to judge in order not to be judged themselves."
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tryptkaloids
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Thems the ones!
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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tripster30
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Ill definitely check these out ..i think with alot more reading i can figure it out .. I have alot of prints to practice with
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IdiotCircusBoy
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Re: First time with agar [Re: tripster30]
#27042747 - 11/16/20 07:06 PM (3 years, 2 months ago) |
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-------------------- Just call me Idiot "People hasten to judge in order not to be judged themselves."
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