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JohnBravo
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Registered: 06/24/19
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Bacteria in bags?
#27040548 - 11/15/20 01:07 PM (3 years, 2 months ago) |
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Hello. These bags were done from agar wedges in front of a flowhood. Since I'm quite busy, I outsource many of the steps, grain preparation included. The grain bags were millet purchased sterilized from an Etsy dealer whom Ive used in the past with no issues.
I was wondering if the bags look bacterially contaminated? If so, do you think the grains were overhydrated?
These bags were going to be master grain bags to transfer to other bags. If the bottom is bacterial, could I still potentially use some of the healthy mycelium grains from the top for transfer? Thank you.
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JohnBravo
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Re: Bacteria in bags? [Re: JohnBravo]
#27041177 - 11/15/20 08:10 PM (3 years, 2 months ago) |
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Bump. Any input?
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LogicaL Chaos
Ascension Energy & Alien UFOs




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Re: Bacteria in bags? [Re: JohnBravo]
#27041207 - 11/15/20 08:29 PM (3 years, 2 months ago) |
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I see some bacteria in there. And yes, they are overhydrated.
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JohnBravo
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Thanks for the reply. So then I imagine even the top grains wouldn't be viable for g2g transfer either? Bleh.
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sandman420
Saint PP



Registered: 06/17/04
Posts: 5,384
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Re: Bacteria in bags? [Re: JohnBravo]
#27041639 - 11/16/20 05:56 AM (3 years, 2 months ago) |
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You really want to mix your bags up before this stage better. You don't want there to be long distance empty runs the mycelium has to colonize. Usually one shake is plenty at about 30% completion. These small bags are kinda a bitch to mix up.
This bottom mushy area is PROBABLY where the bags touch the water or pc. It's not a big deal.
Carefully mix the bags up, making sure to mix the uncolonized part around with the colonized part. If in 72 hours in is not recovering and looking good then it is bacterial. No questions needed.
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Pearl
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Registered: 05/28/20
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Yep, bacterial and over hydrated. Don't use for G2G.
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Bacteria in bags? [Re: JohnBravo]
#27041666 - 11/16/20 06:29 AM (3 years, 2 months ago) |
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 That thick ice cream looking mycelium looks bacterial. It doesn't look healthy. I would not g2g that. You'll know when you shake it the mycelium will be tenacious and won't break apart easily, recovery will be poor.
Edit* this is how you want your mycelium to look. It is differentiated as apposed to one solid/thick mass. You want to see the hypael filaments radiating outwards from the source of inoculation. Pictured below. The creamy texture in your bags is indicative of bacteria.

Edited by Stipe-n Cap (11/16/20 07:01 AM)
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JohnBravo
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Thanks everyone! The risk of outsourcing and not being able to control every step as I used to I suppose.
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thegr8ecb
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Registered: 11/16/20
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Re: Bacteria in bags? [Re: JohnBravo]
#27041710 - 11/16/20 07:14 AM (3 years, 2 months ago) |
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Quote:
JohnBravo said: Hello. These bags were done from agar wedges in front of a flowhood. Since I'm quite busy, I outsource many of the steps, grain preparation included. The grain bags were millet purchased sterilized from an Etsy dealer whom Ive used in the past with no issues.
I was wondering if the bags look bacterially contaminated? If so, do you think the grains were overhydrated?
These bags were going to be master grain bags to transfer to other bags. If the bottom is bacterial, could I still potentially use some of the healthy mycelium grains from the top for transfer? Thank you.

Those bags a fine bro. The black specs are just from the grains it not bacterial.
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Bacteria in bags? [Re: thegr8ecb]
#27041725 - 11/16/20 07:21 AM (3 years, 2 months ago) |
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Quote:
thegr8ecb said: Those bags a fine bro. The black specs are just from the grains it not bacterial.
No, you are wrong Mr 2 posts. Those bags are fucked. The black spots are grain. Lol.
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thegr8ecb
Stranger

Registered: 11/16/20
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Last seen: 3 years, 2 months
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Quote:
p9hu7 said:
Quote:
thegr8ecb said: Those bags a fine bro. The black specs are just from the grains it not bacterial.
No, you are wrong Mr 2 posts. Those bags are fucked. The black spots are grain. Lol.
Quote:
p9hu7 said:
Quote:
thegr8ecb said: Those bags a fine bro. The black specs are just from the grains it not bacterial.
No, you are wrong Mr 2 posts. Those bags are fucked. The black spots are grain. Lol.
Yes, those spots are just the grains poking through.
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thegr8ecb
Stranger

Registered: 11/16/20
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Re: Bacteria in bags? [Re: thegr8ecb]
#27041780 - 11/16/20 07:59 AM (3 years, 2 months ago) |
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Quote:
thegr8ecb said:
Quote:
p9hu7 said:
Quote:
thegr8ecb said: Those bags a fine bro. The black specs are just from the grains it not bacterial.
No, you are wrong Mr 2 posts. Those bags are fucked. The black spots are grain. Lol.
Quote:
p9hu7 said:
Quote:
thegr8ecb said: Those bags a fine bro. The black specs are just from the grains it not bacterial.
No, you are wrong Mr 2 posts. Those bags are fucked. The black spots are grain. Lol.
Yes, those spots are just the grains poking through.
You see?
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thegr8ecb
Stranger

Registered: 11/16/20
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Re: Bacteria in bags? [Re: JohnBravo]
#27041790 - 11/16/20 08:09 AM (3 years, 2 months ago) |
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Quote:
JohnBravo said: Hello. These bags were done from agar wedges in front of a flowhood. Since I'm quite busy, I outsource many of the steps, grain preparation included. The grain bags were millet purchased sterilized from an Etsy dealer whom Ive used in the past with no issues.
I was wondering if the bags look bacterially contaminated? If so, do you think the grains were overhydrated?
These bags were going to be master grain bags to transfer to other bags. If the bottom is bacterial, could I still potentially use some of the healthy mycelium grains from the top for transfer? Thank you.

Don't worry bro. That guy tried to tell me my cake was stressed and full of contams. Which he was WRONG. You do have one bag that looks funny. It's the B+ bag in the third pic it had a bad spot in it. The others are just the grain poking through.
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Bacteria in bags? [Re: thegr8ecb]
#27041795 - 11/16/20 08:12 AM (3 years, 2 months ago) |
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What's your point? Yes, the bag is full of grain. The grain is over hydrated, the grain is bacterial as evidenced by the mycelium. I wouldn't even spawn these bags let alone g2g them.
Excellent job pointing out grains in a bag literally full of grain
I've not commented on your grows but if your eye for contamination is this bad I'd wager that your own grows are equally as fucked.
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thegr8ecb
Stranger

Registered: 11/16/20
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Last seen: 3 years, 2 months
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Quote:
p9hu7 said: What's your point? Yes, the bag is full of grain. The grain is over hydrated, the grain is bacterial as evidenced by the mycelium. I wouldn't even spawn these bags let alone g2g them.
Excellent job pointing out grains in a bag literally full of grain
Quote:
p9hu7 said: What's your point? Yes, the bag is full of grain. The grain is over hydrated, the grain is bacterial as evidenced by the mycelium. I wouldn't even spawn these bags let alone g2g them.
Excellent job pointing out grains in a bag literally full of grain
I've not commented on your grows but if your eye for contamination is this bad I'd wager that your own grows are equally as fucked.
Remember this cake you said was stressed and contaminated ... well, you were WRONG.
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Bacteria in bags? [Re: thegr8ecb]
#27041802 - 11/16/20 08:19 AM (3 years, 2 months ago) |
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No I don't remember, and thats not a fucking cake. Let's see the fruit of your labor.
There is no argument here. The bags in this post ARE bacterial. I've made a clear and concise argument for why they are bacterial, I've even shown how his mycelium should look.
Can you articulate your position in such a way as to disprove my statements? Or are you just going to shout noob nonsense ?
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thegr8ecb
Stranger

Registered: 11/16/20
Posts: 14
Last seen: 3 years, 2 months
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Quote:
p9hu7 said: No I don't remember, and thats not a fucking cake. Let's see the fruit of your labor.
There is no argument here. The bags in this post ARE bacterial. I've made a clear and concise argument for why they are bacterial, I've even shown how his mycelium should look.
Can you articulate your position in such a way as to disprove my statements? Or are you just going to shout noob nonsense ?
Well u have a bad memory then.
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Bacteria in bags? [Re: thegr8ecb]
#27041820 - 11/16/20 08:28 AM (3 years, 2 months ago) |
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So your answer is no. You cannot articulate why his bags are uncontaminated by bacteria. You don't know what you're talking about, you have zero experience and worse still, you are incapable of civil discourse.
I remember things worth remembering.
Apologies OP, this guy loves to derail topics. I won't entertain him any further. Good luck with your grows
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thegr8ecb
Stranger

Registered: 11/16/20
Posts: 14
Last seen: 3 years, 2 months
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Quote:
p9hu7 said: So your answer is no. You cannot articulate why his bags are uncontaminated by bacteria. You don't know what you're talking about, you have zero experience and worse still, you are incapable of civil discourse.
I remember things worth remembering.
Apologies OP, this guy loves to derail topics. I won't entertain him any further. Good luck with your grows
I don't want to discourse with you. You are a fear-monger and also reports people to the Moderators on here like a little snitch.
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Bacteria in bags? [Re: thegr8ecb]
#27041842 - 11/16/20 08:39 AM (3 years, 2 months ago) |
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Lol. I've never done that in my history of being a member of this site. You clearly have me mistaken for someone else. You have gone off topic. You're lucky I'm not a mod. I'm sure one will come by sooner or later though.
Giving out sound direction is far from fear mongering. I have years of experience across many different species. I run a small mushroom farm. You have nothing to back up what you're saying here. You have no experience and zero capability to refute my diagnosis.
Be civil, be on topic, or a mod will set you straight.
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