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seaklate
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First monotub is not fruiting simultaneously and has more and more aborts
#27040693 - 11/15/20 02:35 PM (3 years, 2 months ago) |
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Hi dear ladies and gents,
after two failed attempts with obviously contaminated and multiplied spawn, the first monotub with B+ of a friend is now fruiting. It was inoculated with a spore syringe directly to a spawn bag.
It was made with CVG bulk and a roughly 1:4 ratio, covered with the same CVG coir to help against possible contamination. The CVG ended up with a ph of around 6,5, after being pasteurized for 24 hours in an isolated bucket. The substrate is around two inches high. Otherwise the amount of spawn would have been to less.
It was birthed in a monotub with a sealed lid, traditional 6 hole setup with lower holes taped with duck tape and upper holes with two (and a half diagonal) layers of micropore tape. Colonization took around 12 days with 12 hour light (6500k) when the monotub started pinning without any FAE and around 90 percent colonization.
The pinning was more or less evenly and much stronger than expected. When this set in, FAE was given with one layer of micropore on the upper holes and two layers on the lower ones. The hope was to find a middle way between pinning (that happened anyhow)and further colonization to be safer against contam. After a few days micropore was changed to polyfill mats (as used in kitchen extractor hoods). One layer flat over upper holes, lower holes with rolled pieces. The tightness was varied a bit; first to allow more FAE because of obvious fuzzy feet, then a bit tighter. When (almost) mature fruits would stay very small (only around 3 inches) more FAE was provided to see if it helps.
Pics of the tub (with the bit looser wrapped poly mats):

Lately small fruits/pins (around 1 inch high) started to become shriveled. When cut out with a scalpel these look spongy on the inside. The stems get a bit yellowish/bluish.
See here:

Until now mature fruits were harvested everyday for five days, while the rest is a mix of growing pins and more of these spongy aborts.
The substrate without too much mycelium on it looked a bit light brown and therefore dry. So 100ml water were added with a pipette and soaked up by the substrate. Some of the fuzzy stems also soaked up a bit of it, which can be seen on the pic.
Before adding water:

After adding the water:

So, there are questions on what to do with it:
1. What shall be done with the tub and this first continuous flush? Most of the aborts are cut out in hope other pins will go on to mature. But it seems there are even new pins coming.
2. In respect to have a fresh second flush with a more even fruiting, should at one point everything be cut down completely before misting with the right amount of water (i.e. some 90 percent of harvested mass)?
3. Shall the big stem base compounds also be cut down further to the substrate level? Until now it was avoided to leave other fruits undisturbed.
4. What to do with very small pins? Shall these also be cut away?
and the most important question: what could have caused these aborts and the uneven fruiting?
Help would be very much appreciated:)
edit: added that it is a multispore tub;)
Edited by seaklate (11/15/20 04:10 PM)
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seaklate
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Re: First monotub is not fruiting simultaneously and has more and more aborts [Re: seaklate]
#27042437 - 11/16/20 03:42 PM (3 years, 2 months ago) |
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Bumping in hope to learn something
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A.k.a
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Re: First monotub is not fruiting simultaneously and has more and more aborts [Re: seaklate]
#27042531 - 11/16/20 04:53 PM (3 years, 2 months ago) |
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Kinda looks like they’re left over from the first flush and are now aborting. There’s usually a handful like that.
Judging by the stumps looks like you had a nice first flush.
--------------------
LAGM2020     
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mushhead
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Re: First monotub is not fruiting simultaneously and has more and more aborts [Re: A.k.a]
#27042538 - 11/16/20 04:58 PM (3 years, 2 months ago) |
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This is natural for a tub m8. As it consumes its nutes and continually flushes out it will begin to slowly produce less and less. I would pick off all the fruits and aborts, hydrate, and add a light casing to the top of it to promote myc growth. Then all you need is patience.
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seaklate
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Re: First monotub is not fruiting simultaneously and has more and more aborts [Re: mushhead]
#27043253 - 11/17/20 03:43 AM (3 years, 2 months ago) |
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Thank you for your suggests and info.
The first flush (if one could name it like this) till now is (only?) some 465g wet. One day's harvest was never more than 117g, 58 per day on average, with quite small fruits as mentioned.
It was now tried to cut all obvious aborts. Till today they were more of hundreds than a few. It was also tried to cut the remaining compounds of stumps flatter. Problem is, that all around the substrate and close to these compounds there are hundreds of pins that actually look healty and like waiting to grow. Not sure if they will also abort and become spongy as the other aborts.
Also when trying to flatten the stumps some of these healty pins were hidden in the fuzzy mycelium in it. A few little hats were hurt, so some of the stumps were left as they were .
For sure, one would wait for the latest fruits to become ready to harvest. But can you please advice what to do with the remaining, healty looking pins? As mentioned in the original post, for some reason this fella wants to grow gradually and not in definite stages as expected.
- Shall everything, including healthy looking pins be cut/scraped away before misting/hydrating the tub for a second flush? (a dunk is planned for a possible third flush). If not, it can not be made 100% sure that all aborts can be cut away. There are hundreds, sometimes hidden and very close to healthy pins, and some of the pins could decide to become aborts also.
- Is it risky to have aborts left on the surface for the re-hydration and the second flush?
- Are the stumps as they are now ok to be left for such a major spray/dunk?
- The suggested casing after re-hydration, could it be a dry layer of verm? It is feared any wet casing could cause a perfect enviroment for contaminatins that landed on the surface.
Here is the state of the tub in pictures:
Basically, if you would imagine the few fruits left are away, what would you do?

Thank you so much in advance!
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mushhead
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Re: First monotub is not fruiting simultaneously and has more and more aborts [Re: seaklate]
#27043463 - 11/17/20 08:29 AM (3 years, 2 months ago) |
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You're kind of over thinking this. You need to just cool it, rehydrate, and stop worrying about the ifs and let it happen. Aborts are aborts don't worry about them. Leave them be or not they will not hurt nor hinder you.
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Greens21
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Re: First monotub is not fruiting simultaneously and has more and more aborts [Re: mushhead]
#27043753 - 11/17/20 11:56 AM (3 years, 2 months ago) |
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I definitely agree you’re overthinking a bit. You seem to have a very large number of healthy pins coming up for your second flush, don’t worry about a handful of aborts unless you start seeing them in large scale. Also, a fully colonized and fruiting tub is very healthy and good at fighting off contaminants on its own, adding a wet layer of casing will not harm it; sterile conditions are really only important at the earliest stages of colonization.
It is virtually impossible to remove mature fruits from a tub without also removing some pins or immature fruits when you have different stages of growth like this, don’t worry about it, the energy that would have gone into those shrooms will go into other shrooms later.
Overall, you’re doing great and seem more stressed than you should be. Just enjoy the harvesting
-------------------- I've been trying to justify you In the end I will just defy you
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mushhead
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Re: First monotub is not fruiting simultaneously and has more and more aborts [Re: Greens21]
#27043758 - 11/17/20 12:02 PM (3 years, 2 months ago) |
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seriously.
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Tweeq
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Re: First monotub is not fruiting simultaneously and has more and more aborts [Re: Greens21]
#27043764 - 11/17/20 12:05 PM (3 years, 2 months ago) |
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I'm thinking (maybe) your sub was a lil overhydrated, keeping the fruits smaller. Also, when rehydrating, 90pct of the harvested mass is way too much. Just dink and drain if you think you need to. I'm guessing I would only give the surface a good misting after clearing for 2nd flush
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seaklate
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Re: First monotub is not fruiting simultaneously and has more and more aborts [Re: Tweeq]
#27048395 - 11/20/20 05:47 AM (3 years, 2 months ago) |
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Thank you all for your answers and help!
All close to mature fruits were harvested, (most of the) aborts cut out, then the substrate was hydrated with some 400ml of water after a total yield of 500g so far and considering roughly 100ml that of condensate that was drained before after collecting in the tub (under the lining) during colonization. So, not as much as 90% - as suggested.
The substrate now got a darker look again compared to the very light (dry) look it had before. The few immature fruits left on the substrate seemed to like it and had an instant increase in growth. Overall, the mycelium on the surface seemed to enjoy the re-hydration. Before it became less and the dry spots even looked like they never had been covered. Now it is growing back obviously.
You are right, it can become a spiral of over thinking it. It seems that one can get an enormous amount of information for the stages pre-harvest, but it gets cryptical - or as you supposed just natural - once the tub is in this stage. Reassurance and trust was needed;)
To help others with similar questions and situation, I will demand updated pics and will post them here;)
Thank you very much again!
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