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Offlineblindado
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Registered: 09/10/20
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First time experience - Agar
    #27040420 - 11/15/20 11:46 AM (3 years, 3 months ago)

Hi all, I'm very new to all of this but i tried my luck with agar as I saw that would be the way to go.

Can you please give me your advice on these plates? This is B+ spores from a spore print I bought.

https://drive.google.com/file/d/1jfGHjiyckQ8CuU36nIa6pGer-vXQJg9T/view

https://drive.google.com/file/d/1QWLA4qkORgoEmWXBax3qmhzJH_qlFGE1/view

https://drive.google.com/file/d/1uOm75fZxzwXHhVYZ3u_oUT0gtMUBV0fT/view

EDIT: Maybe my questions are too vague, but I'm such an ignorant that I don't even know yet what to ask.
But, maybe questions like:
Do you see contamination here? I see little fluffy clouds that I don't know if are mold or not. If there is contamination is there any possibility (with the current state of the plates) to do a transfer?


Thanks in advance!


Edited by blindado (11/15/20 12:20 PM)


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InvisibleSir Pentinite
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Registered: 05/15/19
Posts: 525
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Re: First time experience - Agar [Re: blindado]
    #27040470 - 11/15/20 12:21 PM (3 years, 3 months ago)

Upload the pics to this site, not Google Drive.


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- Terrence McKenna


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Offlineblindado
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Re: First time experience - Agar [Re: Sir Pentinite]
    #27040486 - 11/15/20 12:27 PM (3 years, 3 months ago)

Here goes!



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OfflineOne of Us
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Re: First time experience - Agar [Re: blindado]
    #27040665 - 11/15/20 02:13 PM (3 years, 3 months ago)

Its best to streak plates in a zigzag pattern using a sterile swab or inoculation loop. This way it is easier to see what's going on, and you can know where and where not to expect growth.

The plates look pretty dicey, but the organized looking growth on the right of the first picture looks good. The problem is that it is too close to the edge. The edge is often where contams show up, and you can't be sure there aren't any present.

Also, you shouldn't let plates grow out that much before transferring.

I would start over and streak the plate properly. If you want, you could try transferring from the spot I mentioned while waiting for your next plates to germinate.


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