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blindado
Stranger

Registered: 09/10/20
Posts: 2
Last seen: 2 years, 11 months
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First time experience - Agar
#27040420 - 11/15/20 11:46 AM (3 years, 3 months ago) |
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Hi all, I'm very new to all of this but i tried my luck with agar as I saw that would be the way to go.
Can you please give me your advice on these plates? This is B+ spores from a spore print I bought.
https://drive.google.com/file/d/1jfGHjiyckQ8CuU36nIa6pGer-vXQJg9T/view
https://drive.google.com/file/d/1QWLA4qkORgoEmWXBax3qmhzJH_qlFGE1/view
https://drive.google.com/file/d/1uOm75fZxzwXHhVYZ3u_oUT0gtMUBV0fT/view
EDIT: Maybe my questions are too vague, but I'm such an ignorant that I don't even know yet what to ask. But, maybe questions like: Do you see contamination here? I see little fluffy clouds that I don't know if are mold or not. If there is contamination is there any possibility (with the current state of the plates) to do a transfer?
Thanks in advance!
Edited by blindado (11/15/20 12:20 PM)
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Sir Pentinite
Stranger all the time.

Registered: 05/15/19
Posts: 525
Loc: ation Location Location
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Re: First time experience - Agar [Re: blindado]
#27040470 - 11/15/20 12:21 PM (3 years, 3 months ago) |
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Upload the pics to this site, not Google Drive.
-------------------- "I thought to myself 'Boy, I'm sure glad there's nobody here to see this because this is exactly the sort of thing that gets people riled-up and they assume you're dying and that something has to be done. Where if you're alone, you know, you either come through it or you die, but in any case you avoid the fuss.'" - Terrence McKenna
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blindado
Stranger

Registered: 09/10/20
Posts: 2
Last seen: 2 years, 11 months
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One of Us
Stranger



Registered: 03/12/12
Posts: 1,140
Last seen: 25 days, 8 hours
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Re: First time experience - Agar [Re: blindado]
#27040665 - 11/15/20 02:13 PM (3 years, 3 months ago) |
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Its best to streak plates in a zigzag pattern using a sterile swab or inoculation loop. This way it is easier to see what's going on, and you can know where and where not to expect growth.
The plates look pretty dicey, but the organized looking growth on the right of the first picture looks good. The problem is that it is too close to the edge. The edge is often where contams show up, and you can't be sure there aren't any present.
Also, you shouldn't let plates grow out that much before transferring.
I would start over and streak the plate properly. If you want, you could try transferring from the spot I mentioned while waiting for your next plates to germinate.
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