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Shop: Kraken Kratom Red Vein Kratom   North Spore Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies

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OfflineBuho colorido
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I'm a teapot

Registered: 02/18/18
Posts: 241
Last seen: 2 years, 9 months
Problem with sterilizing grains
    #27039857 - 11/15/20 02:23 AM (3 years, 2 months ago)

Hey there, have been gone a long time and am back to cultivation now.
I have problems with sterilizing my grains and want to ask if someone got any ideas.
I'm sterilizing my grains in pint jars in a pressure cooker for 2hrs after pressure is build up.
In the past this worked no problems, but now I see bacterial contamination after about 6-7 days. The bacterial spots start at single grains somewhere in the jar. Even in control jars I didn't inoculate or shake.
My guess is, since there is no gauge on my pressure cooker, that maybe there is not enough pressure built up in my PC. Is there a way I can test if there is 15psi reached?

Agar works with no problems but since it's a liquid, thats no proof for anything I guess.

Any ideas?
I could try sterilizing for a longer period of time but I'm afraid of running dry.
Or I could try fractionated sterilization after 24 hrs.

Any ideas where I can get big PCs like Presto23qt or AA for a reasonable price in Europe ?


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Since english is not my native language, please excuse any misunderstandings or stange verbalizations :wink:


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OfflineHaywire
Wetspot Wizard
Male


Registered: 12/29/13
Posts: 1,611
Last seen: 4 days, 21 hours
Re: Problem with sterilizing grains [Re: Buho colorido]
    #27039866 - 11/15/20 02:38 AM (3 years, 2 months ago)

I got my AA from ebay, shipped from the US of A.
cost me an arm and a leg but no regrets so far.
by far the best product on the market.


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Ciao mamma, guarda come mi diverto


My grows :mushroom2: Outdoor patches


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